数字农科院2.0

Ospkp Alpha 1 Encodes A P.lastidic Pyruvate Kinase That A ffects Starch Biosynthesis In The Rice Endosperm

文献类型： 外文期刊

作者： Cai, Y; Zhang, WW; Jin, J; Yang, XM; You, XM; Yan, HG; Wang, L; Chen, J; Xu, JH; Chen, WW; Chen, XG; Ma, J; Tang, XJ; Kong, F; Zhu, XP; Wang, GX; Jiang, L; Terzaghi, W; Wang, CM; Wan, JM

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期刊名称： JOURNAL OF INTEGRATIVE PLANT BIOLOGY

ISSN： 1672-9072

年卷期： 2018 年 60 卷 11 期

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收录情况： JCR（2021版） ; CSCD（2020-2021年度） ; 科技核心（2020版）

摘要： Pyruvate kinase (PK) is a key enzyme in glycolysis and carbon metabolism. Here, we isolated a rice (Oryza sativa) mutant, w59, with a white-core floury endosperm. Map-based cloning of w59 identified a mutation in OsPKp alpha 1, which encodes a plastidic isoform of PK (PKp). OsPKp alpha 1 localizes to the amyloplast stroma in the developing endosperm, and the mutation of OsPKp alpha 1 in w59 decreases the plastidic PK activity, resulting in dramatic changes to the lipid biosynthesis in seeds. The w59 grains were also characterized by a marked decrease in starch content. Consistent with a decrease in number and size of the w59 amyloplasts, large empty spaces were observed in the central region of the w59 endosperm, at the early grain-filling stage. Moreover, a phylogenetic analysis revealed four potential rice isoforms of OsPKp. We validated the in vitro PK activity of these OsPKps through reconstituting active PKp complexes derived from inactive individual OsPKps, revealing the heteromeric structure of rice PKps, which was further confirmed using a protein-protein interaction analysis. These findings suggest a functional connection between lipid and starch synthesis in rice endosperm amyloplasts.

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