数字农科院2.0

Fast Mapping Of A Chlorophyll B. Synthesis-Deficiency Gene In B arley (Hordeum Vulgare L.) Via Bulked-Segregant Analysis With Reduced-Representation Sequencing

文献类型： 外文期刊

作者： Xu, DD; Sun, D; Diao, YL; Liu, MX; Gao, J; Wu, B; Yuan, XM; Lu, P; Zhang, ZW; Zhang, J; Guo, GG

作者机构：

关键词： Barley; BSA-seq; Reduced representative sequencing; Pale-green; Chlorophyllide a oxygenase

期刊名称： CROP JOURNAL

ISSN： 2095-5421

年卷期： 2019 年 7 卷 1 期

页码：

收录情况： JCR（2021版） ; CSCD（2020-2021年度） ; 农林核心（2020版） ; 科技核心（2020版）

摘要： Bulked-segregant analysis coupled with next-generation sequencing (BSA-seq) has emerged as an efficient tool for genetic mapping of single genes or major quantitative trait loci controlling (agronomic) traits of interest. However, such a mapping-by-sequencing approach usually relies on deep sequencing and advanced statistical methods. Application of BSA-Seq based on construction of reduced-representation libraries and allele frequency analysis permitted anchoring the barley pale-green (pg) gene on chromosome 3HL. With further marker-assisted validation, pg was mapped to a 3.9 Mb physical-map interval. In the pg mutant a complete deletion of chlorophyllide a oxygenase (HvCAO) gene was identified. Because the product of this gene converts Chl a to Chl b, the pg mutant is deficient in Chl b. An independent Chl b-less mutant line M4437_2 carried a nonsynonymous substitution (F263L) in the C domain of HvCAO. The study demonstrates an optimized pooling strategy for fast mapping of agronomically important genes using a segregating population. (C) 2018 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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