文献类型: 外文期刊
作者: Li, Zhiyong;Xin, Xiangdong;Gui, Zhongzheng;Gui, Zhongzheng;Zhao, Shan;Zhang, Bei;Yin, Juan
作者机构:
关键词: Cordyceps militaris; Bisulfite sequencing; DNA methylation; Degenerative; Analysis
期刊名称: ARCHIVES OF MICROBIOLOGY
ISSN: 0302-8933
年卷期: 2019 年 201 卷 3 期
页码:
摘要: The entomopathogenic mushroom Cordyceps militaris is a storehouse of various medicinal compounds and pharmacological effects. However, the high frequency of strain degeneration during subculture and preservation severely limits the large-scale production of C. militaris. DNA methylation is an important epigenomic modification involved in gene regulation. In this study, we used bisulfite sequencing for DNA methylation profiling of wild-type and mutant C. militaris. The differentially methylated regions (DMRs) of the two types were analyzed using Gene Ontology (GO) clustering and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. DNA methylation levels of the wild-type and mutant-type C. militaris were 0.48% and 0.56%, respectively. Methylation appeared at CHH dinucleotides in 58.62% and 58.20% of all methylated cytosine sites in the wild and mutant types, respectively. In all, 188 DMRs were identified from the wild and mutant types. Most of the DMRs ranged from 200 to 350bp in length. KEGG pathways of the expression of DMR-related genes, which are involved in pyruvate metabolism, glycerophospholipid metabolism, DNA replication, and N-glycan biosynthesis. This contributes to the knowledge and understanding of the possible mechanisms of C. militaris strain degeneration.
分类号:
- 相关文献
作者其他论文 更多>>
-
Global Protein Interactome Mapping in Rice Using Barcode-Indexed PCR Coupled with HiFi Long-Read Sequencing
作者:Liu, Xixi;Xia, Dandan;Luo, Jinjin;Li, Mengyuan;Chen, Lijuan;Chen, Yiting;Huang, Jie;Li, Yanan;Xu, Huayu;Yuan, Yang;Cheng, Yu;Li, Zhiyong;Li, Guanghao;Wang, Shiyi;Liu, Xinyong;Liu, Wanning;Zhang, Fengyong;Liu, Zhichao;Tong, Xiaohong;Hou, Yuxuan;Wang, Yifeng;Ying, Jiezheng;Ugli, Abdullaev Mirtemir Baxodir;Ergashev, Mukhammadjon Arabboevich;Zhang, Sanqiang;Yuan, Wenya;Xue, Dawei;Zhang, Jianwei;Zhang, Jian
关键词:barcode-indexed PCR;HiFi sequencing;protein-protein interactome;rice
-
Non-Random Distribution of EMS-Induced Mutations Reveals Preference for Open Chromatin and Expressed Genes in Rice
作者:Yao, Xue-Feng;Liu, Yanhong;Li, Zhiyong;Jiang, Guo-Qiang;Wang, Wensheng;Lu, Hong;Li, Huihui;Lu, Zefu;Liu, Chun-Ming
关键词:chromatin accessibility;EMS mutagenesis;natural variations
-
Transcription Factor OsNF-YC12 Positively Regulates Meloidogyne graminicola Resistance in Rice through OsSRZ22 Activation
作者:Chen, Dong;Li, Zhiyong;Wang, Minghao;Zhao, Wei;Qin, Tong;Liang, Xiaoyan;Huang, Jingjing;Zhao, Jianlong;Zhang, Xiaoxiao;Yang, Shanshan
关键词:Meloidogyne graminicola;Oryzasativa;NF-YC12;defense signaling pathways
-
Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics
作者:Wolella, Eyob Kassaye;Cheng, Zhen;Li, Mengyuan;Xia, Dandan;Zhang, Jianwei;Duan, Liu;Liu, Li;Li, Zhiyong;Zhang, Jian
关键词:rice (Oryza sativa L.);mutagenesis;loss-of-function;gain-of-function;mutant library and DNA barcoding
-
Exogenous abscisic acid represses rice flowering via SAPK8-ABF1- Ehd1 / Ehd2 pathway
作者:Tang, Liqun;Li, Guanghao;Wang, Huimei;Zhao, Juan;Li, Zhiyong;Liu, Xixi;Shu, Yazhou;Liu, Wanning;Wang, Shuang;Huang, Jie;Ying, Jiezheng;Tong, Xiaohong;Yuan, Wenya;Wei, Xiangjin;Tang, Shaoqing;Wang, Yifeng;Bu, Qingyun;Zhang, Jian
关键词:Abscisic acid;bZIP;Flowering;Polycomb Repressive Complex 2 (PRC2);Rice(Oryza sativa L.);SnRK2
-
Contemporary biodiversity pattern is affected by climatechange at multiple temporal scales in steppes on the Mongolian Plateau
作者:Li, Zijing;Li, Zhiyong;Tong, Xuze;Dong, Lei;Zheng, Ying;Zhang, Jinghui;Miao, Bailing;Wang, Lixin;Zhao, Liqing;Wen, Lu;Han, Guodong;Li, Frank Yonghong;Liang, Cunzhu
关键词:
-
Quantification of bioactive components and evaluation of microbial community and antibacterial activity from Heterotrigona itama and Tetrigona binghami honeys
作者:Wu, Jiao;Han, Bingjun;Chen, Xiaoyu;Gao, Jinglin;Zhao, Shan;Sun, Liping;Wang, Shijie
关键词:Antibacterial activity;bioactive compounds;microbial community;stingless bee honey