数字农科院2.0

Bacillus Velezensis Wall Teichoic Acids A.re Required For Biofilm F ormation And Root Colonization

文献类型： 外文期刊

作者： Xu, ZH; Zhang, HH; Sun, XL; Liu, Y; Yan, WX; Xun, WB; Shen, QR; Zhang, RF

作者机构：

关键词： Bacillus velezensis SQR9; UDP-glucose; biofilm formation; root colonization; wall teichoic acids

期刊名称： APPLIED AND ENVIRONMENTAL MICROBIOLOGY

ISSN： 0099-2240

年卷期： 2019 年 85 卷 5 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Rhizosphere colonization by plant growth-promoting rhizobacteria (PGPR) along plant roots facilitates the ability of PGPR to promote plant growth and health. Thus, an understanding of the molecular mechanisms of the root colonization process by plant-beneficial Bacillus strains is essential for the use of these strains in agriculture. Here, we observed that an sfp gene mutant of the plant growth-promoting rhizobacterium Bacillus velezensis SQR9 was unable to form normal biofilm architecture, and differential protein expression was observed by proteomic analysis. A minor wall teichoic acid (WTA) biosynthetic protein, GgaA, was decreased over 4-fold in the Delta sfp mutant, and impairment of the ggaA gene postponed biofilm formation and decreased cucumber root colonization capabilities. In addition, we provide evidence that the major WTA biosynthetic enzyme GtaB is involved in both biofilm formation and root colonization. The deficiency in biofilm formation of the Delta gtaB mutant may be due to an absence of UDP-glucose, which is necessary for the synthesis of biofilm matrix exopolysaccharides (EPS). These observations provide insights into the root colonization process by a plant-beneficial Bacillus strain, which will help improve its application as a biofertilizer. IMPORTANCE Bacillus velezensis is a Gram-positive plant-beneficial bacterium which is widely used in agriculture. Additionally, Bacillus spp. are some of the model organisms used in the study of biofilms, and as such, the molecular networks and regulation systems of biofilm formation are well characterized. However, the molecular processes involved in root colonization by plant-beneficial Bacillus strains remain largely unknown. Here, we showed that WTAs play important roles in the plant root colonization process. The loss of the gtaB gene affects the ability of B. velezensis SQR9 to sense plant polysaccharides, which are important environmental cues that trigger biofilm formation and colonization in the rhizosphere. This knowledge provides new insights into the Bacillus root colonization process and can help improve our understanding of plant-rhizobacterium interactions.

分类号：

  • 相关文献

[1]Quorum Sensing Signal Autoinducer-2 Promotes Root Colonization Ofbacillus Velezensissqr9 By Affecting Biofilm Formation And Motility. Xiong, Q, Liu, D, Zhang, HH, Dong, XY, Zhang, GS, Liu, YP, Zhang, RF. 2020

[2]The Phosphotransferase System Gene Ptsh P.lays An Important Role I n Mnsod Production, Biofilm Formation, Swarming Motility, And Root Colonization In Bacillus Cereus 905. Gao, TT, Ding, MZ, Yang, CH, Fan, HY, Chai, YR, Li, Y. 2019

[3]Ftsex-Cwlo Regulates Biofilm Formation By A. Plant-Beneficial Rhizobacterium Bacillus V elezensis Sqr9. Li, Q, Li, ZF, Li, XX, Xia, LM, Zhou, X, Xu, ZH, Shao, JH, Shen, QR, Zhang, RF. 2018

[4]Identification of Adhesins in Plant Beneficial Rhizobacteria Bacillus velezensis SQR9 and Their Effect on Root Colonization. Rong Huang,Haichao Feng,Zhihui Xu,Nan Zhang,Yunpeng Liu,Jiahui Shao,Qirong Shen,Ruifu Zhang. 2022

[5]Bacillus velezensis SQR9: a model biofertilizer strain for beneficial plant root-rhizobacterium interaction. Sun, Xinli,Xu, Zhihui,Zhang, Nan,Liu, Yunpeng,Xun, Weibing,Miao, Youzhi,Shao, Jiahui,Zhang, Ruifu,Shen, Qirong. 2025

[6]Resde Two-Component Regulatory System Mediates O.xygen Limitation-Induced Biofilm Formation B y Bacillus Amyloliquefaciens Sqr9. Zhou, X, Zhang, N, Xia, LM, Li, Q, Shao, JH, Shen, QR, Zhang, RF. 2018

[7]The Phosphotransferase System Gene Ptsi I.n Bacillus Cereus Regulates E xpression Of Soda2 And Contributes To Colonization Of Wheat Roots. Gao, TT, Li, Y, Ding, MZ, Chai, YR, Wang, Q. 2017

[8]Complete Genome Sequence Ofenterobacter Roggenkampiied5, A Nitrogen Fixing Plant Growth Promoting Endophytic Bacterium With Biocontrol And Stress Tolerance Properties, Isolated From Sugarcane Root. Guo, DJ, Singh, RK, Singh, P, Li, DP, Sharma, A, Xing, YX, Song, XP, Yang, LT, Li, YR. 2020

[9]Effects of Arbuscular Mycorrhizal Fungi on Maize Growth, Root Colonization, and Root Exudates Varied with Inoculum and Application Method. Hafiz Athar Hussain,Zhang Qingwen,Saddam Hussain,Li Hongbo,Ahmed Waqqas,Zhang Li. 2021

[10]Pdh Modulate Virulence Through Reducing S.tress Tolerance And Biofilm F ormation Of Streptococcus Suis Serotype 2. Wang, Y, Wang, YX, Liu, BB, Wang, SH, Li, JP, Gong, SL, Sun, LY, Yi, L. 2019

[11]Identification Of Genes Involved In M.ycoplasma Gallisepticum Biofilm Formation U sing Mini-Tn4001-Sgm Transposon Mutagenesis. Wang, Y, Yi, L, Zhang, FQ, Qiu, XS, Tan, L, Yu, SQ, Cheng, XC, Ding, C. 2017

[12]Resistance response to Arenicin derivatives in Escherichia coli. Wang Z., Yang N., Teng D., Hao Y., Li T., Han H., Mao R., Wang J.. 2022

[13]Biosurfactants Of Lactobacillus Helveticus For B.iodiversity Inhibit The Biofilm F ormation Of Staphylococcus Aureus And Cell Invasion. Jiang, XP, Yan, X, Gu, SS, Yang, Y, Zhao, LL, He, XM, Chen, HY, Ge, JW, Liu, D. 2019

[14]Chlorpyrifos Inhibits Nitrogen Fixation In Rice-Vegetated Soil Containing Pseudomonas Stutzeri A1501. Lu, C, Yang, ZM, Liu, J, Liao, QH, Ling, WT, Waigi, MG, Odinga, ES. 2020

[15]Synthesis And Detoxification Of Nitric O.xide In The Plant B eneficial Rhizobacterium Bacillus Amyloliquefaciens Sqr9 And Its Effect On Biofilm Formation. Dong, XY, Liu, YP, Zhang, GS, Wang, DD, Zhou, X, Shao, JH, Shen, QR, Zhang, RF. 2018

作者其他论文 更多>>