数字农科院2.0

Itraq-Based Protein Profiling And Biochemical A.nalysis Of Two Contrasting R ice Genotypes Revealed Their Differential Responses To Salt Stress

文献类型： 外文期刊

作者： Zhu, Chunquan;Zhang, Junhua;Wang, Liping;Riaz, Aamir;Nanda, Satyabrata;Liang, Qingduo;Li, Yefeng;Jin, Qianyu;Hussain, Sajid;Hussain, Saddam;Huang, Jie;Cao, Xiaochuang;Bai, Zhigang;Zhu, Lianfeng

作者机构：

关键词： Salt stress; Oryza sativa; proteomics; iTRAQ quantification; cell membrane injury; root activity

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN： 1422-0067

年卷期： 2019 年 20 卷 3 期

页码：

摘要： Salt stress is one of the key abiotic stresses causing huge productivity losses in rice. In addition, the differential sensitivity to salinity of different rice genotypes during different growth stages is a major issue in mitigating salt stress in rice. Further, information on quantitative proteomics in rice addressing such an issue is scarce. In the present study, an isobaric tags for relative and absolute quantitation (iTRAQ)-based comparative protein quantification was carried out to investigate the salinity-responsive proteins and related biochemical features of two contrasting rice genotypesNipponbare (NPBA, japonica) and Liangyoupeijiu (LYP9, indica), at the maximum tillering stage. The rice genotypes were exposed to four levels of salinity: 0 (control; CK), 1.5 (low salt stress; LS), 4.5 (moderate salt stress; MS), and 7.5 g of NaCl/kg dry soil (high salt stress, HS). The iTRAQ protein profiling under different salinity conditions identified a total of 5340 proteins with 1% FDR in both rice genotypes. In LYP9, comparisons of LS, MS, and HS compared with CK revealed the up-regulation of 28, 368, and 491 proteins, respectively. On the other hand, in NPBA, 239 and 337 proteins were differentially upregulated in LS and MS compared with CK, respectively. Functional characterization by KEGG and COG, along with the GO enrichment results, suggests that the differentially expressed proteins are mainly involved in regulation of salt stress responses, oxidation-reduction responses, photosynthesis, and carbohydrate metabolism. Biochemical analysis of the rice genotypes revealed that the Na+ and Cl- uptake from soil to the leaves via the roots was increased with increasing salt stress levels in both rice genotypes. Further, increasing the salinity levels resulted in increased cell membrane injury in both rice cultivars, however more severely in NPBA. Moreover, the rice root activity was found to be higher in LYP9 roots compared with NPBA under salt stress conditions, suggesting the positive role of rice root activity in mitigating salinity. Overall, the results from the study add further insights into the differential proteome dynamics in two contrasting rice genotypes with respect to salt tolerance, and imply the candidature of LYP9 to be a greater salt tolerant genotype over NPBA.

分类号：

  • 相关文献

[1]Integration Of Proteomic And Transcriptomic P.rofiles Reveals Multiple Levels O f Genetic Regulation Of Salt Tolerance In Cotton. Geng, Xiaoli,He, Shoupu,Jia, Yinhua,Pan, Zhaoe,Gong, Wenfang,Xu, Feifei,Peng, Zhen,Du, Xiongming. 2018

[2]Effects Of Metalaxyl Enantiomers Stress O.n Root Activity And L eaf Antioxidant Enzyme Activities In Tobacco Seedlings. Fang, S, Tao, Y, Zhang, YZ, Kong, FY, Wang, YB. 2018

[3]Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress. ZhongQun, He,ChaoXing, He,ZhiBin, Zhang,ZhiRong, Zou,HuaiSong, Wang.

[4]N-6-Methyladenine Dna Methylation In Japonica A.nd Indica Rice Genomes A nd Its Association With Gene Expression, Plant Development, And Stress Responses. Wang, Yunpeng,Riaz, Adeel,Lu, Tiegang,Liu, Min,Liu, Jingrong,Liang, Zhe,Liang, Zhe,Li, Yun,Ji, Changmian,Gu, Xiaofeng,Cui, Xuean,Yang, Donglei,Yao, Pu,Yu, Hao,Yu, Hao,Zhang, Pingxian,Zheng, Hongkun,Zhang, Qian. 2018

[5]The Dnaj Protein Osdja6 Negatively R.egulates Rice Innate Immunity T o The Blast Fungus Magnaporthe Oryzae. Wang, Guo-Liang,Shi, Yanlong,Zhong, Xionghui,Yang, Jiuxia,Wang, Xuli,Wang, Guo-Liang. 2018

[6]Heat Stress Induces Spikelet Sterility I.n Rice At Anthesis T hrough Inhibition Of Pollen Tube Elongation Interfering With Auxin Homeostasis In Pollinated Pistils. Feng, Baohua,Tao, Longxing,Chen, Tingting,Fu, Guanfu,Fu, Weimeng,Li, Guangyan,Zhang, Caixia,Jin, Qianyu,Yan, Jinxiang,Islam, Mohammad Rezaul. 2018

[7]Studies On The Efficiency Of D.ifferent Inoculation Methods Of R ice White-Tip Nematode, Aphelenchoides Besseyi. Peng, Yunliang,Peng, Yunliang,Wang, Yanping,Ji, Hongli,Xie, Jialian,Yang, Fang. 2019

[8]Association Study Reveals Genetic Loci R.esponsible For Arsenic, Cadmium A nd Lead Accumulation In Rice Grain In Contaminated Farmlands. Chen, Sunlu,Qin, Ping,Zheng, Guangyong,Xu, Xiangyang,Shi, Xiaoliang,Teng, Sheng,Peng, Yu,Liu, Xiuyan,Liu, Xiuyan,Liu, Xiuyan,Chen, Sunlu,Chen, Mingxue. 2019

[9]Biochemical And Molecular Characterizations Of S.alt And Phytohormones-Induced Changes I n Roots And Shoots Of Rice Seedlings. Xu, Mengyun,Jamil, Muhammad,Jamil, Muhammad,Wang, Wenyi. 2018

[10]Sodium Chloride Stress During Early G.rowth Stages Altered Physiological A nd Growth Characteristics Of Rice. Hussain, Sajid,Khaskheli, Maqsood Ahmad,Fiaz, Sajid,Cao, Xiaochuang,Zhu, Lianfeng,Zhong, Chu,Zhang, Junhua,Jin, Qianyu. 2018

[11]The Oshapl1-Dth8-Hd1 Complex Functions As T.he Transcription Regulator To R epress Heading Date In Rice. Zhu, SS, Wang, JC, Cai, MH, Zhang, H, Wu, FQ, Xu, Y, Li, CN, Cheng, ZJ, Zhang, X, Guo, XP, Sheng, PK, Wu, MM, Wang, JL, Lei, CL, Wang, J, Zhao, ZC, Wu, CY, Wang, HY, Wan, JM. 2017

[12]Characterization Of A Rice Dwarf A.nd Narrow Leaf 2 M utant. Adedze, YMN, Wei, XJ, Sheng, ZH, Jiao, GA, Tang, SQ, Hu, PS. 2017

[13]Tsv, A Putative Plastidic Oxidoreductase, P.rotects Rice Chloroplasts From C old Stress During Development By Interacting With Plastidic Thioredoxin Z. Sun, J, Zheng, TH, Yu, J, Wu, TT, Wang, XH, Chen, GM, Tian, YL, Zhang, H, Wang, YH, Terzaghi, W, Wang, CM, Wan, JM. 2017

[14]Flowering Time Regulation By The C.onstans-Like Gene Oscol10. Tan, JJ, Wu, FQ, Wan, JM. 2017

[15]Disruption Of Ossec3A Increases The C.ontent Of Salicylic Acid A nd Induces Plant Defense Responses In Rice. Ma, J, Chen, J, Wang, M, Ren, YL, Wang, S, Lei, CL, Cheng, ZJ, Sodmergen. 2018

[16]Floury Endosperm8, Encoding The Udp-Glucose P.yrophosphorylase 1, Affects The S ynthesis And Structure Of Starch In Rice Endosperm. Long, WH, Dong, BN, Wang, YH, Pan, PY, Wang, YL, Liu, LL, Chen, XL, Liu, X, Liu, SJ, Tian, YL, Chen, LM, Wan, JM. 2017

[17]Osarm1, An R2R3 Myb Transcription F.actor, Is Involved In R egulation Of The Response To Arsenic Stress In Rice. Wang, FZ, Chen, MX, Yu, LJ, Xie, LJ, Yuan, LB, Qi, H, Xiao, M, Guo, WX, Chen, Z, Yi, KK, Zhang, JH, Qiu, RL, Shu, WS, Xiao, S, Chen, QF. 2017

[18]Floury Endosperm11 Encoding A Plastid H.eat Shock Protein 70 I s Essential For Amyloplast Development In Rice. Zhu, XP, Teng, X, Wang, YL, Hao, YY, Jing, RN, Wang, YF, Liu, Y, Zhu, JP, Wu, MM, Zhong, MS, Chen, XL, Zhang, YY, Zhang, WW, Wang, CM, Wang, YH, Wan, JM. 2018

[19]Minor-Effect Qtl For Heading Date D.etected In Crosses Between I ndica Rice Cultivar Teqing And Near Isogenic Lines Of Ir24. Sun, ZC, Zhu, YJ, Chen, JY, Zhang, H, Zhang, ZH, Niu, XJ, Fan, YY, Zhuang, JY. 2018

[20]Wsl5, A Pentatricopeptide Repeat Protein, I.s Essential For Chloroplast B iogenesis In Rice Under Cold Stress. Liu, X, Lan, J, Huang, YS, Cao, PH, Zhou, CL, Ren, YK, He, NQ, Liu, SJ, Tian, YL, Nguyen, T, Jiang, L, Wan, JM. 2018

作者其他论文 更多>>