数字农科院2.0

Short-Term Inhibition Of Glutamine Synthetase L.eads To Reprogramming Of A mino Acid And Lipid Metabolism In Roots And Leaves Of Tea Plant (Camellia Sinensis L.)

文献类型： 外文期刊

作者： Liu, MY; Tang, DD; Shi, YZ; Ma, LF; Li, Y; Zhang, QF; Ruan, JY

作者机构：

关键词： Glutamine synthetase; Methionine sulfoximine; Quality-related compounds; Amino acids; Lipids; Tea plant

期刊名称： BMC PLANT BIOLOGY

ISSN： 1471-2229

年卷期： 2019 年 19 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Background Nitrogen (N) nutrition significantly affected metabolism and accumulation of quality-related compounds in tea plant (Camellia sinensis L.). Little is known about the physiological and molecular mechanisms underlying the effects of short-term repression of N metabolism on tea roots and leaves for a short time. Results In this study, we subjected tea plants to a specific inhibitor of glutamine synthetase (GS), methionine sulfoximine (MSX), for a short time (30 min) and investigated the effect of the inhibition of N metabolism on the transcriptome and metabolome of quality-related compounds. Our results showed that GS activities in tea roots and leaves were significantly inhibited upon MSX treatment, and both tissue types showed a sensitive metabolic response to GS inhibition. In tea leaves, the hydrolysis of theanine decreased with the increase in theanine and free ammonium content. The biosynthesis of all other amino acids was repressed, and the content of N-containing lipids declined, suggesting that short-term inhibition of GS reduces the level of N reutilization in tea leaves. Metabolites related to glycolysis and the tricarboxylic acid (TCA) cycle accumulated after GS repression, whereas the content of amino acids such as glycine, serine, isoleucine, threonine, leucine, and valine declined in the MXS treated group. We speculate that the biosynthesis of amino acids is affected by glycolysis and the TCA cycle in a feedback loop. Conclusions Overall, our data suggest that GS repression in tea plant leads to the reprogramming of amino acid and lipid metabolic pathways.

分类号：

  • 相关文献

[1]Cytoplasmic Glutamine Synthetase Gene Expression R.egulates Larval Development In B actrocera Dorsalis (Hendel). Wei, Dong,Wei, Dong,Zhang, Meng-Yi,Zhang, Meng-Yi,Wang, Jin-Jun,Jia, Hong-Ting,Jia, Hong-Ting,Wang, Jin-Jun,Wei, Dong,Liu, Yu-Wei,Smagghe, Guy,Taning, Clauvis Nji Tizi,Liu, Yu-Wei,Smagghe, Guy,Li, Ran,Li, Ran,Smagghe, Guy. 2018

[2]Comprehensive School-Based Intervention To Control O.verweight And Obesity In C hina: A Cluster Randomized Controlled Trial. Du, Songming,Liu, Ailing,Liu, Weijia,Ma, Guansheng,Guo, Hongwei,Ma, Guansheng,Ma, Jun,Li, Yanping,Li, Ying,Li, Yanping,Li, Tingyu,Hu, Xiaoqi,Xu, Guifa,Xu, Haiquan,Xu, Haiquan,Zhang, Qian. 2017

[3]Food-Grade Encapsulation Systems For (-)-Epigallocatechin G.allate. Zheng, Xin-Qiang,Li, Xu-Min,Shi, Yun-Long,Ye, Jian-Hui,Yang, Rui,Liang, Yue-Rong,Ma, Shi-Cheng,Shi, Meng,Lu, Jian-Liang,Cai, Zhuo-Yu,Li, Qing-Sheng. 2018

[4]Identification and Expression Analysis of Glutamine Synthetase Genes in Ramie (Boehmeria nivea L. Gaud). Zheng, Jian-Shu,Yu, Chun-Ming,Chen, Ping,Wang, Yan-Zhou,Liu, Tou-Ming,Xiong, He-Ping. 2015

[5]Characterization of a glutamine synthetase gene BnGS1-2 from ramie (Boehmeria nivea L. Gaud) and biochemical assays of BnGS1-2-over-expressing transgenic tobacco. Zheng, Jian-Shu,Yu, Chun-Ming,Chen, Ping,Wang, Yan-Zhou,Tan, Long-Tao,Chen, Ji-Kang,Xiong, He-Ping.

[6]Arabidopsis cytosolic glutamine synthetase AtGLN1;1 is a potential substrate of AtCRK3 involved in leaf senescence. Li, RJ,Hua, W,Lu, YT.

[7]Haplotype analysis of the genes encoding glutamine synthetase plastic isoforms and their association with nitrogen-use- and yield-related traits in bread wheat. Li, Xin-Peng,Zhao, Xue-Qiang,He, Xue,Li, Bin,Liu, Dong-Cheng,Zhang, Ai-Min,Tong, Yi-Ping,Li, Zhen-Sheng,Zhao, Guang-Yao,Zhang, Xue-Yong. 2011

[8]Involvement of GlnK, a P(II) protein, in control of nitrogen fixation and ammonia assimilation in Pseudomonas stutzeri A1501. He, Sheng,Chen, Ming,Xie, Zhihong,Yan, Yongliang,Li, Hongquan,Fan, Ying,Ping, Shuzhen,Lin, Min,He, Sheng,Elmerich, Claudine. 2008

[9]Molecular analyses of tomato GS, GOGAT and GDH gene families and their response to abiotic stresses. Liu, Lifeng,Wang, Jing,Han, Zhihui,Li, Hanxia,Zhang, Junhong,Lu, Yongen,Liu, Lifeng,Sun, Xuecheng.

[10]Characterization of key lipids for binding and generating aroma compounds in roasted mutton by UPLC-ESI-MS/MS and Orbitrap Exploris GC. Liu H., Hui T., Zheng X., Li S., Wei X., Li P., Zhang D., Wang Z.. 2022

[11]Linking Gut Microbiome And Lipid Metabolism: Moving Beyond Associations. Lamichhane, S, Sen, P, Alves, MA, Ribeiro, HC, Raunioniemi, P, Hyotylainen, T, Oresic, M. 2021

[12]Cloning, Expression, And Nutritional Regulation O.f The Glutamine Synthetase G ene In Ctenopharyngodon Idellus. Hu, R, Qu, FF, Tang, JZ, Zhao, Q, Yan, JP, Zhou, ZG, Zhou, Y, Liu, Z. 2017

[13]Effect Of Steamed Potato Bread Intake On Glucose, Lipids, And Urinary Na+ And K+: A Randomized Controlled Trial With Adolescents. Xu, HQ, Guo, YZ, Lu, SJ, Ma, YQ, Wang, XL, Zhao, LY, Sun, JM. 2020

[14]Conservation tillage for 17 years alters the molecular composition of organic matter in soil profile. Qiqi Gao,Lixiao Ma,Yunying Fang,Aiping Zhang,Guichun Li,Junjian Wang,Di Wu,Wenliang Wu,Zhangliu Du. 2021

[15]Evaluation Of The Potential Of C.hicoric Acid As A N atural Food Antioxidant. Zhu, XL, Huang, FH, Xiang, X, Fan, M, Chen, TT. 2018

[16]Systematic characterization of Gossypium GLN family genes reveals a potential function of GhGLN1.1a regulates nitrogen use efficiency in cotton. Xiaotong Li,Yunqi Gu,Mirezhatijiang Kayoumu,Noor Muhammad,Xiangru Wang,Huiping Gui,Tong Luo,Qianqian Wang,Xieraili Wumaierjiang,Sijia Ruan,Asif Iqbal,Xiling Zhang,Meizhen Song,Qiang Dong. 2024

[17]Unraveling the mechanisms of multiple resistance across glyphosate and glufosinate in Eleusine indica. Jingchao Chen,Bin Shan,Zhiling Li,Qian Chen,Haiyan Yu,Hailan Cui,Xiangju Li. 2024

[18]Transcriptomic And Metabolomic Profiling Of D.rought-Tolerant And Susceptible Sesame G enotypes In Response To Drought Stress. Zhang, Yujuan,Dossa, Komivi,Zhang, Xiurong,Yu, Jingyin,Zhang, Yanxin,Zhou, Rong,Zhang, Yujuan,Liu, Aili,Li, Donghua,Wang, Linhai,You, Jun,Wang, Xiao,Dossa, Komivi. 2019

[19]Light-Sensitive Albino Tea Plants And T.heir Characterization. Shi, Yun-Long,Liang, Yue-Rong,Ma, Shi-Cheng,Zheng, Xin-Qiang,Lu, Jian-Liang,Fu, Qiu-Yue,Shin, Young-Hwan,Wang, Kai-Rong,Ye, Jian-Hui,Li, Xu-Min,Yang, Rui. 2018

[20]Identifying Metabolic Perturbations And Toxic Effects Of Rac-Metalaxyl And Metalaxyl-M In Mice Using Integrative Nmr And Uplc-Ms/Ms Based Metabolomics. Wang, Sheng,He, Lin,Zhu, Wentao,Zhang, Ping,Zhang, Ping,Wang, Sheng,Xu, Yangyang,He, Yuhan,He, Yuhan,Yang, Furong,Yu, Weizhong,Shi, Dongmei,Xu, Yangyang,Zhang, Ping,Zhang, Ping,He, Lin,Zhang, Ping,Zhang, Ping,Yang, Furong,Yu, Weizhong,Shi, Dongmei,He, Yuhan,He, Lin. 2019

作者其他论文 更多>>