数字农科院2.0

Nfis, A Species-Specific Regulatory Noncoding R.na Of Pseudomonas Stutzeri, E nhances Oxidative Stress Tolerance In Escherichia Coli

文献类型： 外文期刊

作者： Yan, Yongliang;Hu, Guihua;Hu, Guihua;Huang, Yunhong;Lin, Min;Hu, Tao;Zhan, Yuhua;Lu, Wei

作者机构：

关键词： Pseudomonas stutzeri A1501; NfiS; Recombinant Escherichia coli; Oxidative stress; Regulatory element

期刊名称： AMB EXPRESS

ISSN： 2191-0855

年卷期： 2019 年 9 卷 1 期

页码：

摘要： Noncoding RNAs (ncRNAs) can finely control the expression of target genes at the posttranscriptional level in prokaryotes. Regulatory small RNAs (sRNAs) designed to control target gene expression for applications in metabolic engineering and synthetic biology have been successfully developed and used. However, the effect on the heterologous expression of species- or strain-specific ncRNAs in other bacterial strains remains poorly understood. In this work, a Pseudomonas stutzeri species-specific regulatory ncRNA, NfiS, which has been shown to play an important role in the response to oxidative stress as well as osmotic stress in P. stutzeri A1501, was cloned and transferred to the Escherichia coli strain Trans10. Recombinant NfiS-expressing E. coli, namely, Trans10-nfiS, exhibited significant enhancement of tolerance to oxidative stress. To map the possible gene regulatory networks mediated by NfiS in E. coli under oxidative stress, a microarray assay was performed to delineate the transcriptomic differences between Trans10-nfiS and wild-type E. coli under H2O2 shock treatment conditions. In all, 1184 genes were found to be significantly altered, and these genes were divided into mainly five functional categories: stress response, regulation, metabolism related, transport or membrane protein and unknown function. Our results suggest that the P. stutzeri species-specific ncRNA NfiS acts as a regulator that integrates adaptation to H2O2 with other cellular stress responses and helps protect E. coli cells against oxidative damage.

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