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The Cadherin Cry1Ac Binding-Region Is N.ecessary For The Cooperative E ffect With Abcc2 Transporter Enhancing Insecticidal Activity Of Bacillus Thuringiensis Cry1Ac Toxin

文献类型： 外文期刊

作者： Ma, Yuemin;Soberon, Mario;Peng, Rong;Xiao, Yutao;Bravo, Alejandra;Liu, Chenxi;Zhang, Jianfeng;Liu, Kaiyu;Yang, Yanchao;Yang, Yongbo

作者机构：

关键词： Helicoverpa armigera; Spodoptera litura; cadherin; ABCC2 transporter; Bacillus thuringiensis; synergism; Cry1Ac

期刊名称： TOXINS

ISSN：

年卷期： 2019 年 11 卷 9.0 期

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收录情况： JCR（2021版）

摘要： Bacillus thuringiensis Cry1Ac toxin binds to midgut proteins, as cadherin (CAD) and ABCC2 transporter, to form pores leading to larval death. In cell lines, co-expression of CAD and ABCC2 enhance Cry1Ac toxicity significantly, but the mechanism remains elusive. Here, we show that the expression of Helicoverpa armigera CAD (HaCAD-GFP) in Hi5 cells induces susceptibility to Cry1Ac and enhanced Cry1Ac toxicity when co-expressed with H. armigera ABCC2 (HaABCC2-GFP), since Cry1Ac toxicity increased 735-fold compared to Hi5 cells expressing HaCAD-GFP alone or 28-fold compared to HaABCC2-GFP alone. In contrast, the expression of the Spodoptera litura CAD (SlCAD-GFP) in Hi5 cells did not induce susceptibility to Cry1Ac nor it potentiated Cry1Ac toxicity with HaABCC2-GFP. To identify the CAD regions involved in the enhancement of Cry1Ac toxicity with ABCC2, the different CAD domains were replaced between SlCAD-GFP and HaCad-GFP proteins, and cytotoxicity assays were performed in Hi5 cells in the absence or presence of HaABCC2-GFP. The HaCAD toxin-binding region (TB), specifically the CAD repeat-11, was necessary to enhance Cry1Ac toxicity with ABCC2. We propose that CAD TB is involved in recruiting Cry1Ac to localize it in a good position for its interaction with the ABCC2, resulting in efficient toxin membrane insertion enhancing Cry1Ac toxicity.

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