数字农科院2.0

A Crispr-Cas12A-Derived Biosensing Platform For T.he Highly Sensitive Detection O f Diverse Small Molecules

文献类型： 外文期刊

作者： Zhang, Qian;Karthik, Loganathan;Li, Chuan;Shi, Tong;Yu, Jiaming;Shuai, Yuting;Zhu, Guoliang;Wang, Ke:Feng;Yu, Jiaming;Yang, Zhiheng;Liu, Chenli;Zhang, Lu;Yang, Zhiheng;Zhang, Lu;Shi, Tong;Zhang, Qian;Li, Shanshan;Liu, Xueting;Zhou, Liming;Zhang, Buchang;Liang, Mindong;Liu, Guang;Liu, Leshi;Zhang, Jingyu;Zhuo, Ying;Liu, Jiakun;Yu, Jing;Xia, Xuekui;Wang, Zhong;Tan, Gao:Yi;Wang, Man;Liang, Mindong;Zhang, Li:Xin;Xie, Feng;Li, Zilong;Zhu, Guoliang;Liang, Mindong;Liu, Jiakun;Liu, Leshi;Dai, Huanqin;Karthik, Loganathan;Wang, Ke:Feng;Liu, Jiakun;Li, Zilong;Dai, Huanqin;Wang, Weishan;Xie, Feng;Li, Chuan;Shuai, Yuting;Wang, Weishan

作者机构：

期刊名称： NATURE COMMUNICATIONS

ISSN： 2041-1723

年卷期： 2019 年 10 卷

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收录情况： JCR（2021版）

摘要： Besides genome editing, CRISPR-Cas12a has recently been used for DNA detection applications with attomolar sensitivity but, to our knowledge, it has not been used for the detection of small molecules. Bacterial allosteric transcription factors (aTFs) have evolved to sense and respond sensitively to a variety of small molecules to benefit bacterial survival. By combining the single-stranded DNA cleavage ability of CRISPR-Cas12a and the competitive binding activities of aTFs for small molecules and double-stranded DNA, here we develop a simple, supersensitive, fast and high-throughput platform for the detection of small molecules, designated CaT-SMelor (CRISPR-Cas12a-and aTF-mediated small molecule detector). CaT-SMelor is successfully evaluated by detecting nanomolar levels of various small molecules, including uric acid and p-hydroxybenzoic acid among their structurally similar analogues. We also demonstrate that our CaT-SMelor directly measured the uric acid concentration in clinical human blood samples, indicating a great potential of CaT-SMelor in the detection of small molecules.

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