数字农科院2.0

Effect Of In Vitro Sub-Culture O.f Sandy Pear On V ariant Structure Of Apple Stem Grooving Virus

文献类型： 外文期刊

作者： Hu, GJ; Wang, GP; Hong, N

作者机构：

关键词： Apple stem grooving virus (ASGV); In vitro culture; Single-strand conformation polymorphism; Virus variants

期刊名称： TROPICAL PLANT PATHOLOGY

ISSN： 1983-2052

年卷期： 2019 年 44 卷 3 期

页码：

收录情况： JCR（2021版）

摘要： In vitro culture of sand pear (Pyrus pyrifolia) cv. Huanghua (HH) infected with apple stem grooving virus (ASGV) was conducted and the titer and variant mutations of the virus in sub-culture were analyzed. The results showed that ASGV could replicate stably in 20 successive sub-cultures. The 500bp PCR products of ASGV in each sub-culture were cloned and used for single-strand conformation polymorphism analysis. The results demonstrated that there was a dominant molecular variant of ASGV in vitro and that the variant structure of ASGV is stable in sub-culturing. The rate of the main haplotype in the 20 passages was from 78.0% to 95.7%. To clarify the origin of subordinate haplotypes, the error rate of our RT-PCR assay (5.4x 10(-4)/nt) was computed and the results indicated that the mutations which appeared in sub-passages were acquired from artificial operation and not produced by viral replication.

分类号：

  • 相关文献

[1]Elimination Of Apple Stem Pitting V.irus From In Vitro-Cultured P ear By An Antiviral Agent Combined With Thermotherapy. Hu, GJ, Hong, N, Wang, GP. 2019

[2]In Vitro Culture And Biological P.roperties Of Broiler Adipose-Derived S tem Cells. Lu, TF, Pei, WH, Wang, KF, Zhang, S, Chen, FH, Wu, YN, Guan, WJ. 2018

[3]Effect Of Pre-Culture On Virus E.limination From In Vitro A pple By Thermotherapy Coupled With Shoot Tip Culture. Hu, GJ, Dong, YF, Zhang, ZP, Fan, XD, Ren, F, Li, ZN. 2018

[4]Isolation And Characterization Of Extracellular V.esicles From Adult Schistosoma J aponicum. Liu, JT, Zhu, LH, Wang, LH, Chen, YJ, Gin, BR, Li, JJ, Cheng, GF. 2018

作者其他论文 更多>>