数字农科院2.0

Alkaline Phosphatase 2 Is A F.unctional Receptor Of Cry1Ac B ut Not Cry2Ab In Helicoverpa Zea

文献类型： 外文期刊

作者： Wei, J.;Li, X.;Wei, J.;Li, X.;Wei, J.;Liang, G.;Zhang, M.;Wei, J.;Zhang, M.;Zhang, M.

作者机构：

关键词： Bt-transgenic crops; cell toxicity; cross-resistance; gain-of-function analysis; pyramid; RNAi

期刊名称： INSECT MOLECULAR BIOLOGY

ISSN： 0962-1075

年卷期： 2019 年 28 卷 3.0 期

页码：

收录情况： JCR（2021版）

摘要： Although membrane-bound alkaline phosphatases (ALPs) have been proposed as a receptor for Cry1Ac in a few lepidopteran species, their potential functions as a Cry2Ab receptor are yet to be verified. To determine if ALP2 also serves as a receptor for Cry1Ac and even for Cry2Ab in Helicoverpa zea, we measured the potency of activated Cry1Ac and Cry2Ab against midgut and fat body cell lines of H. zea and the ovarian cell line of Spodoptera frugiperda (Sf9) expressing H. zea ALP2 (HzALP2) or transfected with HzALP2 double-stranded RNA (dsRNA). Relative to the control cells, the three cell lines expressing HzALP2 were more susceptible to Cry1Ac but there was no difference for Cry2Ab. By contrast, the two H. zea cell lines transfected with HzALP2 dsRNA were resistant to Cry1Ac while kept susceptible to Cry2Ab. Furthermore, RNA interference knockdown of HzALP2 in H. zea larvae enhanced larval survival on Cry1Ac-containing diets. These findings indicate that HzALP2 functions as a receptor of Cry1Ac but not Cry2Ab.

分类号：

  • 相关文献

[1]Cytotoxicity And Binding Profiles Of A.ctivated Cry1Ac And Cry2Ab T o Three Insect Cell Lines. Guo, Yuyuan,Gu, Shaohua,Li, Xianchun,Li, Xianchun,Li, Xianchun,Li, Xianchun,Wei, Jizhen,Li, Xianchun,Liang, Gemei,Ni, Xinzhi,Wei, Jizhen,Wu, Kongming,Wei, Jizhen. 2018

[2]Resistant Inheritance And Cross-Resistance Of C.yflumetofen In Tetranychus Cinnabarinus ( Boisduval). Wei, Peng,Wei, Peng,Shi, Li,Wen, Xiang,Yang, Yuwei,Wen, Xiang,Yang, Yuwei,He, Lin,He, Xiulong,Feng, Kaiyang,Feng, Kaiyang,Shi, Li,He, Xiulong,He, Lin. 2018

[3]The Cytochrome P450 Cyp389C16 Contributes T.o The Cross-Resistance Between C yflumetofen And Pyridaben In Tetranychus Cinnabarinus (Boisduval). Ou, Shiyuan,Zhang, Ping,Yang, Yuwei,Feng, Kaiyang,Hu, Yuan,Hu, Yuan,Wen, Xiang,Yang, Yuwei,Xu, Zhifeng,Wen, Xiang,Feng, Kaiyang,Zhang, Ping,Ou, Shiyuan,Zhang, Yichao,Shen, Guangmao,Xu, Zhifeng,Shen, Guangmao,He, Lin,Shi, Li,He, Lin. 2019

[4]Non-Target-Site And Target-Site Resistance To A.has Inhibitors In American S loughgrass (Beckmannia Syzigachne). Wang Jing-jing,Yu Hui-lin,Li Zheng,Li Xiang-ju,Li Dan,Han Yu-jiao,Cui Hai-lan. 2018

[5]Development And Relative Fitness Of C.ry1C Resistance In Chilo S uppressalis. Chen, Geng,Han, Lanzhi,Peng, Yufang,Tang, Hui,Tang, Hui,Chen, Fajun. 2018

[6]Characterisation Of Imidacloprid Resistance In T.he Bird Cherry-Oat Aphid, R hopalosiphum Padi, A Serious Pest On Wheat Crops. Wang, K, Zhang, M, Huang, YN, Yang, ZL, Su, S, Chen, MH. 2018

[7]Variation In Mutations Providing Resistance To Acetohydroxyacid Synthase Inhibitors In Cyperus Difformis In China. Li, Z, Li, XJ, Chen, JC, Peng, LC, Wang, JJ, Cui, HL. 2020

[8]Downregulation Of Carboxylesterase Contributes To C.yflumetofen Resistance In Tetranychus C innabarinus (Boisduval). Nan, Can,Nan, Can,He, Lin,Shen, Guangmao,Wei, Peng,Wei, Peng,Shen, Guangmao,Cheng, Jiqiang,Chen, Ming,Chen, Ming,Cheng, Jiqiang,He, Lin,Feng, Kaiyang,Feng, Kaiyang. 2019

[9]Characterization Of A New Bunyavirus A.nd Its Derived Small R nas In The Brown Citrus Aphid, Aphis Citricidus. Ye, Chao,Yang, Li,Zhang, Wei,Zhang, Wei,Ye, Chao,Niu, Jinzhi,Niu, Jinzhi,Tan, Yang,Chang, Tengyu,Guo, Mengmeng,Guo, Mengmeng,Tan, Yang,Chang, Tengyu,Wu, Tengfei,Yang, Li,Wang, Jin-Jun,Wang, Jin-Jun,Wu, Tengfei. 2019

[10]Ecdysis Triggering Hormone Signaling (Eth/Ethr-A) I.s Required For The L arva-Larva Ecdysis In Bactrocera Dorsalis (Diptera: Tephritidae). Pei, Yu-Xia,Liu, Xiao-Qiang,Gui, Shun-Hua,Jiang, Hong-Bo,Shi, Yan,Smagghe, Guy,Xu, Li,Smagghe, Guy,Wang, Jin-Jun,Smagghe, Guy,Jiang, Hong-Bo,Wang, Jin-Jun,Shi, Yan,Gui, Shun-Hua,Liu, Xiao-Qiang,Pei, Yu-Xia,Xu, Li. 2017

[11]The Ecdysis Triggering Hormone System, V.ia Eth/Ethr-B , Is E ssential For Successful Reproduction Of A Major Pest Insect, Bactrocera Dorsalis (Hendel). Shi, Yan,Smagghe, Guy,Smagghe, Guy,Wang, Jin-Jun,Smagghe, Guy,Smagghe, Guy,Dou, Wei,Jiang, Hong-Bo,Dou, Wei,Dou, Wei,Dou, Wei,Wang, Jin-Jun,Park, Yoonseong,Liu, Tian-Yuan,Jiang, Hong-Bo,Liu, Xiao-Qiang,Liu, Xiao-Qiang,Shi, Yan,Wang, Jin-Jun,Smagghe, Guy,Wang, Jin-Jun,Jiang, Hong-Bo,Liu, Tian-Yuan,Jiang, Hong-Bo. 2019

[12]Vitellogenin And Its Receptor Play E.ssential Roles In The D evelopment And Reproduction Of The Brown Citrus Aphid, Aphis (Toxoptera) Citricidus. Shang, F.,Niu, J. -Z.,Ye, C.,Zhang, Q.,Shang, F.,Ding, B. -Y.,Niu, J. -Z.,Ding, B. -Y.,Ye, C.,Wang, J. -J.,Smagghe, G.,Smagghe, G.,Smagghe, G.,Wang, Jin -Jun,Zhang, Q.,Zhang, W.,Zhang, W.. 2018

[13]Expression Analysis And Functional Identification O.f Several Genes Related T o Diapause In Bombyx Mori. Tang, Shunming,Tang, Shunming,Tan, Zhichen,Shen, Xingjia,Qian, Ping,Shen, Xingjia,Chen, Yanhua,Jiang, Tao,Qian, Ping,Li, Jiashuang,Jiang, Tao. 2019

[14]Crispr-S: An Active Interference Element F.or A Rapid And I nexpensive Selection Of Genome-Edited, Transgene-Free Rice Plants. Huang, Jian-Zhong,Huang, Jian-Zhong,Liu, Song-Mei,Shu, Qing-Yao,Tan, Yuan-Yuan,Lu, Hai-Ping,Lu, Hai-Ping,Chen, Wu-Yang,Shu, Qing-Yao,Tan, Yuan-Yuan,Chen, Wu-Yang,Shu, Qing-Yao,Huang, Jian-Zhong,Xu, Shou-Ling,Zhou, Xin. 2017

[15]Aac As A Potential Target G.ene To Control Verticillium D ahliae. Rehman, Latifur,Su, Xiaofeng,Zhang, Rui,Cheng, Hongmei,Guo, Huiming,Li, Xiaokang. 2017

[16]Functional Characterization Of Bdb1, A W.ell-Conserved Carboxylesterase Among Tephritid F ruit Flies Associated With Malathion Resistance In Bactrocera Dorsalis (Hendel). Wang, Luo-Luo,Meng, Li-Wei,Wang, Luo-Luo,Wang, Jin-Jun,Wang, Jin-Jun,Lu, Xue-Ping,Smagghe, Guy,Smagghe, Guy,Smagghe, Guy,Lu, Xue-Ping,Meng, Li-Wei. 2017

[17]Genetic Enhancement Of Palmitic Acid A.ccumulation In Cotton Seed O il Through Rnai Down-Regulation Of Ghkas2 Encoding Beta-Ketoacyl-Acp Synthase Ii (Kasii). Wu, Man,Singh, Surinder P.,Wu, Man,Zhang, Baolong,Zhang, Baolong,Liu, Qing,Green, Allan G.,Liu, Qing,Shrestha, Pushkar,Petrie, James. 2017

[18]Functional Analysis Of Four Upregulated C.arboxylesterase Genes Associated With F enpropathrin Resistance In Tetranychus Cinnabarinus (Boisduval). Shi, Li,Liu, Xinyang,Wei, Peng,Shi, Li,Liu, Xinyang,He, Lin,Li, Jinhang,Nan, Can,Shi, Li,Li, Jinhang,Li, Chuanzhen,Li, Chuanzhen,He, Lin,Wei, Peng,Zhang, Yichao,Zhang, Yichao,Nan, Can. 2019

[19]Expression And Evolutionary Analyses Of T.hree Acetylcholinesterase Genes (Mi-Ace-1, M i-Ace-2, Mi-Ace-3) In The Root-Knot Nematode Meloidogyne Incognita. Peng, Deliang,Huang, Wenkun,Sun, Xiaotang,Zhang, Lei,Wu, Qingsong,Cui, Ruqiang,Chen, Yuyan,Cui, Ruqiang,Fan, Chengming,da Silva, Washington. 2017

[20]Improving Zinc And Iron Accumulation I.n Maize Grains Using T he Zinc And Iron Transporter Zmzip5. Chen, Rumei,Yang, Wenzhu,Li, Ye,Liu, Xiaoqing,Li, Suzhen,Zhou, Xiaojin. 2019

作者其他论文 更多>>