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Complete Genome Sequencing Of Bacillus V.elezensis Wrn014, And Comparison W ith Genome Sequences Of Other Bacillus Velezensis Strains

文献类型: 外文期刊

作者: Wang, JR; Xing, JY; Lu, JK; Sun, YJ; Zhao, JJ; Miao, SH; Xiong, Q; Zhang, YG; Zhang, GS

作者机构:

关键词: Bacillus velezensis; comparative genomics; phylogenetic analysis; gene clusters

期刊名称: JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

ISSN: 1017-7825

年卷期: 2019 年 29 卷 5 期

页码:

收录情况: JCR(2021版)

摘要: Bacillus velezensis strain WRN014 was isolated from banana fields in Hainan, China. Bacillus velezensis is an important member of the plant growth-promoting rhizobacteria (PGPR) which can enhance plant growth and control soil-borne disease. The complete genome of Bacillus velezensis WRN014 was sequenced by combining Illumina Hiseq 2500 system and Pacific Biosciences SMRT high-throughput sequencing technologies. Then, the genome of Bacillus velezensis WRN014, together with 45 other completed genome sequences of the Bacillus velezensis strains, were comparatively studied. The genome of Bacillus velezensis WRN014 was 4,063,541bp in length and contained 4,062 coding sequences, 9 genomic islands and 13 gene clusters. The results of comparative genomic analysis provide evidence that (i) The 46 Bacillus velezensis strains formed 2 obviously closely related clades in phylogenetic trees. (ii) The pan-genome in this study is open and is increasing with the addition of new sequenced genomes. (iii) Analysis of single nucleotide polymorphisms (SNPs) revealed local diversification of the 46 Bacillus velezensis genomes. Surprisingly, SNPs were not evenly distributed throughout the whole genome. (iv) Analysis of gene clusters revealed that rich gene clusters spread over Bacillus velezensis strains and some gene clusters are conserved in different strains. This study reveals that the strain WRN014 and other Bacillus velezensis strains have potential to be used as PGPR and biopesticide.

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