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Enhanced Efficacy Of A Multi-Epitope Vaccine For Type A And O Foot-And-Mouth Disease Virus By Fusing Multiple Epitopes With Mycobacterium Tuberculosis Heparin-Binding Hemagglutinin (Hbha), A Novel Tlr4 Agonist

文献类型： 外文期刊

作者： Lei, Y; Shao, JJ; Ma, FF; Lei, CL; Chang, HY; Zhang, YG

作者机构：

关键词： FMDV; HBHA; TLR4 agonist; Multi-epitope vaccine

期刊名称： MOLECULAR IMMUNOLOGY

ISSN： 0161-5890

年卷期： 2020 年 121 卷

页码：

收录情况： JCR（2021版）

摘要： Foot-and-mouth disease (FMD) is an acute, severe, and highly contagious disease that affects cloven-hoofed animals and can lead to serious economic losses and social effects. Therefore, a safe and effective subunit vaccine is required to prevent and control FMD. Dendritic cells (DCs) are a type of professional antigen presenting cell (APC). Immature DCs are typically stimulated by various adjuvants via immune receptors (e.g., toll-like receptor 4 [TLR4]), which activate DCs to induce their maturation. TLR4 has been well-established to induce both innate and adaptive immune responses to various external microbial or internal damage-related molecular patterns. In this study, the multi-epitope immunogen, HAO, of foot-and-mouth disease virus (FMDV) serotypes A and O was fused with the recombinant protein, heparin-binding hemagglutinin (HBHA), a novel TLR4 agonist, to obtain a new recombinant fusion protein, termed HAO-HBHA. HAO-HBHA was found to be highly efficient at activating murine DCs by the TLR4 pathway, both in vitro and in vivo. HAO-HBHA elicited strong specific humoral immune responses detected with an ELISA and virus neutralizing antibody test (VNT). HAO-HBHA also elevated the cellular immune responses, as indicated by intracellular cytokine (e.g., IFN-gamma, TNF-alpha, IL-4, IL-6, IL-10, and IL-12p70) expression in Th1 and Th2 cells. As a TLR4 agonist, HBHA has significant advantages for enhancing the immune efficacy of a FMDV serotype A and O bivalent multi-epitope vaccine. These findings provide a novel strategy for the development of a safe and effective multi-epitope vaccine candidate against FMDV and further extends the application of TLR agonist-based vaccine platforms.

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