Adenovirus-Vectored Capsid Proteins Of The Serotype A Foot-And-Mouth Disease Virus Protect Guinea Pigs Against Challenge
文献类型: 外文期刊
作者: Xie, YL; Chang, HY; Li, ZY; Zhang, YM
作者机构:
关键词: Foot-And-Mouth Disease Virus; Capsid Proteins; Recombinant Adenovirus; Mutated Viral 3C Protease; Cytokines; Iga Antibody
期刊名称: FRONTIERS IN MICROBIOLOGY
ISSN: 1664-302X
年卷期: 2020 年 11 卷
页码:
收录情况: JCR(2021版)
摘要: Type A foot-and-mouth disease virus (FMDV) has been detected on China's pig farms since 2015, and all suspected samples have been strain A/GDMM/CHA/2013. To overcome the shortcomings of inactive FMDV vaccines, we expressed the capsid protein precursor P1-2A and mutated viral 3C protease of FMDV strain A/GDMM/CHA/2013 in a replication-deficient human adenovirus type 5 vector in this study. A significant humoral immune response, T-cell-mediated antiviral response, and mucosa-mediated antiviral response were induced by the adenovirus-vectored FMDV vaccines in BALB/c mice. Immunization of guinea pigs with the adenovirus-vectored FMD vaccines induced significant neutralizing antibodies and anti-FMDV immunoglobulin A antibodies. The recombinant adenovirus rAdv-P12A3C(G38SF48S)-GD protected 100% of guinea pigs against challenge when administered intramuscularly. Our study demonstrated the potential utility of rAdv-P12A3C(G38SF48S)-GD as a vaccine against type A FMDV.
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