数字农科院2.0

Preparation And Application Of Monoclonal A.ntibody To Sugarcane (Saccharum L . Spp. Hybrids) Sop5Cs Protein

文献类型: 外文期刊

作者: Li, Y. R.;Wang, L. R.;Yang, L. T.;Xing, Y. X.;Huang, X.;Li, Y. R.;Huang, X.;Li, Y. R.;Li, J.;Huang, X.

作者机构:

关键词: sugarcane; P5CS gene; proline; monoclonal antibody; physiological index

期刊名称: APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH

ISSN: 1589-1623

年卷期: 2019 年 17 卷 5 期

页码:

摘要: Delta(1)-pyrroline-5-carboxylate synthase (P5CS) is a key enzyme in proline biosynthesis. In this study, the nucleotide sequence of sugarcane P5CS (SoP5CS) gene was obtained from sugarcane. Then the ORF of SoP5CS linked with pET-30a vector and the SoP5CS protein was expressed successfully in E. coli by the induction of 1.0 mM IPTG at 28 degrees C for 6 h. The prokaryotic expression protein was expressed as inclusion bodies (IBs). It was purified by Ni-NTA Resin firstly and then refolded, dialyzed and further purified by gel filtration. The monoclonal antibody of SoP5CS was prepared using mice. The expression of SoP5CS gene and the corresponding protein and physiological parameters associated with drought resistance were detected in sugarcane leaves under different drought stresses, they worked together to regulate the drought resistance process of sugarcane. This is the first report on the preparation of P5CS monoclonal antibody and the expression of the antibody in sugarcane, which would provide new methods to study the function of SoP5CS response to various abiotic stresses and other aspects.

分类号:

  • 相关文献

[1]Isolation, Transformation And Overexpression Of S.ugarcane Sop5Cs Gene For D rought Tolerance Improvement. Li, Yang-Rui,Li, Jian,Yang, Li-Tao,Thi-Thu Phan,Xing, Yong-Xiu. 2018

[2]Butachlor-Induced Alterations In Ultrastructure, Antioxidant, A.nd Stress-Responsive Gene Regulations I n Rice Cultivars. Wang, Jian,Wang, Jian,Islam, Faisal,Islam, Faisal,Zhu, Jinwen,Zhu, Jinwen,Zhou, Weijun,Zhou, Weijun,Gill, Rafaqat A.,Ali, Skhawat,Ali, Skhawat,Ali, Basharat,Ali, Basharat,Farooq, Muhammad A.,Gill, Rafaqat A.,Farooq, Muhammad A.,Farooq, Muhammad A.. 2017

[3]5-Aminolevulinic Acid Improves Salt Tolerance M.ediated By Regulation Of T etrapyrrole And Proline Metabolism In Brassica Napus L. Seedlings Under Nacl Stress. Xiong, JL, Wang, HC, Tan, XY, Zhang, CL, Naeem, MS. 2018

[4]Characterization Of Chinese Unifloral Honeys B.ased On Proline And P henolic Content As Markers Of Botanical Origin, Using Multivariate Analysis. Wen, YQ, Zhang, JZ, Li, Y, Chen, LZ, Zhao, W, Zhou, JH, Jin, Y. 2017

[5]Transcriptome Profiling, Biochemical And Physiological Analyses Provide New Insights Towards Drought Tolerance In Nicotiana Tabacum L.. Khan, RY, Zhou, PL, Ma, XH, Zhou, L, Wu, YH, Ullah, Z, Wang, SS. 2019

[6]Tea From Organic Production Has H.igher Functional Quality Characteristics C ompared With Tea From Conventional Management Systems In China. Han, WY, Wang, DH, Fu, SW, Ahmed, S. 2018

[7]Overexpression Of Lily Hsfa3S In A.rabidopsis Confers Increased Thermotolerance A nd Salt Sensitivity Via Alterations In Proline Catabolism. Zhong, Xionghui,Wu, Ze,Zhao, Xin,Cao, Xing,Liang, Jiahui,Yi, Mingfang,He, Junna,Wang, Chengpeng,Li, Guoqing. 2018

[8]Assessment Of Castor Plant (Ricinus Communis L.) Tolerance To Heavy Metal Stress-A Review. Yeboah, A, Lu, JN, Yang, T, Shi, YZ, Amoanimaa-Dede, H, Boateng, KGA, Yin, XG. 2020

[9]Biological Roles Of Ornithine Aminotransferase (.Oat) In Plant Stress T olerance: Present Progress And Future Perspectives. Anwar, A, She, MY, Wang, K, Riaz, B, Ye, XG. 2018

[10]The Role Of Ip3 In N.o-Enhanced Chilling Tolerance In P each Fruit. Jiao, CF, Duan, YQ. 2019

[11]Slwrky81 Reduces Drought Tolerance By Attenuating Proline Biosynthesis In Tomato. Ahammed, GJ, Li, X, Wan, HJ, Zhou, GZ, Cheng, Y. 2020

[12]Evaluation Of The Protective Immunity Of Riemerella Anatipestifer Ompa. Xu, XX, Xu, YH, Miao, S, Jiang, P, Cui, JS, Gong, YS, Tan, PP, Du, XL, Islam, N, Hu, QH. 2020

[13]Development And Detection Application Of M.onoclonal Antibodies Against Zucchini Y ellow Mosaic Virus. Zhou Xue-ping,Zhou Xue-ping,Zhang Ming-hao,Chen Zhe,Wu Jian-xiang. 2017

[14]Development Of An Indirect Competitive E.lisa For The Detection O f Acenaphthene And Pyrene. Qiao, B, Li, YS, Meng, XY, Sun, Y, Hu, P, Lu, SY, Ren, HL, Liu, ZS, Zhou, Y. 2017

[15]Determination Of Deoxynivalenol By Elisa A.nd Immunochromatographic Strip Assay B ased On Monoclonal Antibodies. Yon, TT, Zhang, Q, Wang, D, Li, PW, Tang, XQ, Zhang, W. 2019

[16]Expression And Characterization Of A R.ecombinant Porcinized Antibody Against T he E2 Protein Of Classical Swine Fever Virus. Chen, SC, Li, S, Sun, HM, Li, YF, Ji, SW, Song, K, Zhang, LK, Luo, YZ, Sun, Y, Ma, JF, Liu, PH, Qiu, HJ. 2018

[17]Identification And Characterization Of A N.ovel B-Cell Epitope On A leutian Mink Disease Virus Capsid Protein Vp2 Using A Monoclonal Antibody. Lu, TF, Wang, YZ, Ge, JW, Ma, Q, Yan, WZ, Zhang, YY, Zhao, LL, Chen, HY. 2018

[18]Neutralization Of Genotype 2 Porcine E.pidemic Diarrhea Virus Strains B y A Novel Monoclonal Antibody. Liu, JB, Shi, HY, Chen, JF, Zhang, X, Ji, ZY, Yuan, J, Shi, D, Cao, LY, Zhu, XD, Dong, H, Wang, XB, Zhang, JL, Feng, L. 2017

[19]An Ultrasensitive Gray-Imaging-Based Quantitative Immunochromatographic D.etection Method For Fumonisin B -1 In Agricultural Products. Tang, XQ, Li, PW, Zhang, ZW, Zhang, Q, Guo, JN, Zhang, W. 2017

[20]Identification Of A Serotype-Independent Linear E.pitope Of Foot-And-Mouth Disease V irus. Yang, BL, Wang, MX, Liu, WM, Xu, ZQ, Wang, HW, Yang, DC, Ma, WG, Zhou, GH, Yu, L. 2017

作者其他论文 更多>>