数字农科院2.0

Epigallocatechin Gallate (Egcg) Inhibited The A.lv-J-Induced Apoptosis In Df-1 C ells By Inactivation Of Nuclear Factor Kappa B Pathway

文献类型： 外文期刊

作者： Xu, Z.;Deng, M.;Li, Z.;Yang, H.;Wu, X.;Zhang, Y.

作者机构：

关键词： ALV-J; EGCG; NF-kappa B; DF-1 cell; apoptosis

期刊名称： BRAZILIAN JOURNAL OF POULTRY SCIENCE

ISSN： 1516-635X

年卷期： 2019 年 21 卷 1 期

页码：

摘要： Avian leukosis virus subgroup J (ALV-J), a member of the retroviridae family, can infect both broilers and layers and induce a spectrum of different neoplasms, resulting in serious economic losses in poultry production. Epigallocatechin-3-gallate (EGCG), the major constituent of green tea, has demonstrated remarkable anti-inflammatory and cancer chemopreventive effects in many animal tumor bioassays, cell culture systems and epidemiological studies. To assess the antiviral effects of EGCG on ALV-J-induced cell apoptosis in vitro, DF-1 cells were treated with different EGCG concentrations (0, 5, 10, 20 and 40 mu g/mL), and their antiviral effects were examined at different time points (0, 24, 48, 72 and 96 h) using a variety of assays. EGCG alleviated the ALV-J-induced apoptosis in a dose-dependent manner. Because high concentrations (20 and 40 mu g/mL) inhibited DF-1 cell growth, and low concentration (5 mu g/ mL) did not suppress the ALV-J virus, 10 mu g/mL was the most appropriate concentration. After 96 h of incubation, 10 mu g/mL EGCG improved the ALV-J-triggered suppression of the nuclear transcription factor system by enhancing cytoplasmic NF-kappa B p50/p65 expression and inhibiting nuclear NF-kappa B p50/p65 expression, resulting in decreased cell apoptosis. These results demonstrated that EGCG inhibited ALV-J-induced apoptosis in DF-1 cells in a dose-dependent manner via the NE-kappa B signaling pathway, and that 10 mu g/mL EGCG is the optimal concentration, which may be useful for therapeutic drug design.

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