数字农科院2.0

Molecular Characterization And Functional Analysis Of Two New Lysozyme Genes From Soybean Cyst Nematode (Heterodera Glycines)

文献类型： 外文期刊

作者： Peng Huan;Huang Wen-kun;Peng De-liang;Wang Ning;Liu-Clarke, Jihong;Holgado, Ricardo;Liu Shi-ming

作者机构：

关键词： Soybean Cyst Nematode; Lysozyme; Hg-Lys1; Hg-Lys2; Innate Defense Against Bacteria

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2019 年 18 卷 12 期

页码：

摘要： Soybean cyst nematode (SCN, Heterodera glycines (I.)) is one of the most important soil-borne pathogens for soybeans. In plant parasitic nematodes, including SCN, lysozyme plays important roles in the innate defense system. In this study, two new lysozyme genes (Hg-lys1 and Hg-lys2) from SCN were cloned and characterized. The in situ hybridization analyses indicated that the transcripts of both Hg-lys1 and Hg-lys2 accumulated in the intestine of SCN. The qRT-PCR analyses showed that both Hg-lys1 and Hg-lys2 were upregulated after SCN second stage juveniles (J2s) were exposed to the Gram-positive bacteria Bacillus thuringiensis, Bacillus subtilis or Staphylococcus aureus. Knockdown of the identified lysozyme genes by in vitro RNA interference caused a significant decrease in the survival rate of SCN. All of the obtained results indicate that lysozyme is very important in the defense system and survival of SCN.

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