数字农科院2.0

Technical Note: Development And Application Of Kasp Assays For Rapid Screening Of 8 Genetic Defects In Holstein Cattle

文献类型： 外文期刊

作者： Zhang, Y; Liang, D; Huang, HT; Yang, ZC; Wang, YC; Yu, Y; Liu, L; Zhang, SL; Han, JL; Xiao, W

作者机构：

关键词： Allele-Specific Pcr; Genotyping; Genetic Defect; Recessive Allele

期刊名称： JOURNAL OF DAIRY SCIENCE

ISSN： 0022-0302

年卷期： 2020 年 103 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Specific DNA mutations underlying several genetic defects associated with embryo loss or reduced calf survivability have been identified in dairy cattle, and a convenient and cost-effective platform is required for their routine screening. We developed Kompetitive allele-specific PCR (KASP) assays for discrimination of the wild-type alleles from the associated defective alleles at each of 8 common genetic defects in Holstein cattle, involving 5 SNP [HH1, HH3, HH4, bovine leukocyte adhesion deficiency (BLAD), and complex vertebral malformation (CVM)] and 3 insertion or deletion mutations [HH5, haplotype for cholesterol deficiency (HCD), arid brachyspina (BS)]. A total of 390 cows from a Chinese Holstein herd were genotyped and the carriers identified at 7 of these 8 loci (except HH4), with the highest carrier frequencies found for CVM (10.5%) arid HH1 (10.0%), followed by HH3 (2.6%), BS (2.1%), HCD (1.3%), HH5 (0.8%), and BLAD (0.5%). Surprisingly, 102 cows (26.2%) carried at least 1 of the 7 defective alleles. Our results demonstrate that these KASP assays are simple, rapid, arid reliable for the detection of multiple genetic defects. The high carrier frequency of these genetic defects indicates an urgent need for routine molecular testing to eliminate the deleterious alleles from Chinese Holstein cattle.

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