数字农科院2.0

Genetic And Agronomic Traits Stability Of Marker-Free Transgenic Wheat Plants Generated From Agrobacterium-Mediated Co-Transformation In T-2 And T-3 Generations

文献类型： 外文期刊

作者： Liu, HY; Wang, K; Wang, J; Du, LP; Pei, XW; Ye, XG

作者机构：

关键词： Wheat; Marker-Free Transgenic Plants; Fluorescence In Situ Hybridization; Genetic Stability

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2020 年 19 卷 1 期

页码：

收录情况： JCR（2021版） ; CSCD（2020-2021年度） ; 农林核心（2020版） ; 科技核心（2020版）

摘要： Genetically modified wheat has not been commercially utilized in agriculture largely due to regulatory hurdles associated with traditional transformation methods. Development of marker-free transgenic wheat plants will help to facilitate biosafety evaluation and the eventual environmental release of transgenic wheat varieties. In this study, the marker-free transgenic wheat plants previously obtained by Agrobacterium-mediated co-transformation of double T-DNAs vector were identified by fluorescence in situ hybridization (FISH) in the T-1 generation, and their genetic stability and agronomic traits were analyzed in T-2 and T-3 generations. FISH analysis indicated that the transgene often integrated into a position at the distal region of wheat chromosomes. Furthermore, we show that the GUS transgene was stably inherited in the marker-free transgenic plants in T-1 to T-3 generations. No significant differences in agronomic traits or grain characteristics were observed in T-3 generation, with the exception of a small variation in spike length and grains per spike in a few lines. The selection marker of bargene was not found in the transgenic plants through T-1 to T-3 generations. The results from this investigation lay a solid foundation for the potential application of the marker-free transgenic wheat plants achieved through the co-transformation of double T-DNAs vector by Agrobacterium in agriculture after biosafty evaluation.

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