数字农科院2.0

Development And Application Of A Novel Elisa For Detecting Antibodies Against Group I Fowl Adenoviruses

文献类型： 外文期刊

作者： Pan, Q; Wang, J; Gao, YL; Cui, HY; Liu, CJ; Qi, XL; Zhang, YP; Wang, YQ; Li, K; Gao, L; Wang, XM

作者机构：

关键词： Fowl Adenovirus; Elisa; Group-Specific; Fadv-4; Antibody Detection

期刊名称： APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

ISSN： 0175-7598

年卷期： 2020 年 104 卷 2 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Since 2015, outbreaks of hepatitis-hydropericardium syndrome (HPS) caused by a novel genotype of fowl adenovirus 4 (FAdV-4) infection have created serious economic losses in China. Given that other serotypes of hypervirulent FAdVs have also been reported in poultry around the world, a common ELISA for all serotypes within the group I fowl adenoviruses (FAdV-I) is urgently needed, especially for clinical epidemic serotypes. In this study, we used high purity and concentration virions of FAdV-4 and developed a common ELISA for detecting antibodies against 12 FAdV-I serotypes. The developed ELISA was able to distinguish between antibodies against FAdV-I, FAdV-III, and other heterologous viruses without any cross-reaction. Furthermore, the ELISA showed higher sensitivity than the FAdV-1-based ELISA to the novel FAdV-4 found in China. Moreover, since there are no commercial vaccines against FAdVs in China, the ELISA was applied to detect sera samples from specific pathogen-free chickens inoculated with inactivated FAdV-1, FAdV-4, and FAdV-8a. The assay showed high sensitivities for all three detected serotypes within FAdV-I. In conclusion, a novel, common ELISA for FAdV-I was developed in this study and could be a powerful tool for seroepidemiological investigations and FAdVs vaccine development.

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