数字农科院2.0

Mini-Disk Capillary Array Coupling With Lamp For Visual Detection Of Multiple Nucleic Acids Using Genetically Modified Organism Analysis As An Example

文献类型： 外文期刊

作者： Zhang, Xiujie;Chen, Jianwei;Li, Rong;Cui, Jingjie;Yang, Litao;Yang, Litao;Tao, Shengce

作者机构：

关键词： Multiplex Nucleic Acid Testing; Mini-Disk Capillary Array; Loop-Mediated Isothermal Amplification; Visual Detection; Genetically Modified Organism

期刊名称： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN： 0021-8561

年卷期： 2020 年 68 卷 3 期

页码：

摘要： Convenient, portable, and low-cost multiplex nucleic acid testing (NAT) systems are the trends in the fields of food safety, environmental microorganisms, molecular diagnosis, etc. In this study, we developed a novel system for visual monitoring of multiple nucleic acids combining a mini-disk capillary array (diameter = 17 mm, embedded with 6-10 capillaries), visual loop mediated isothermal amplification (LAMP), and quick DNA extraction called mDC-LAMP. The performance and applicability of mDC-LAMP in testing multiple nucleic acids were evaluated and verified employing genetically modified contents analysis as an example. All of the results confirmed that mDC-LAMP has the advantages of high specificity without any cross contamination, high sensitivity with a limit of detection of 25 copies/reaction, high throughput with flexible channel sensors, easy fabrication, and low costs. We believe that mDC-LAMP is a competitive choice for on-spot monitoring of multiple nucleic acids in terms of the easy fabrication/operation, low costs, and suitable performance presented in the nucleic acids test.

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