数字农科院2.0

High Efficient Chemiluminescent Immunoassays For The Detection Of Diclazuril In Chicken Muscle Based On Biotin-Streptavidin System

文献类型: 外文期刊

作者: Li, Yuan;Zhang, Yingjie;Li, PeiPei;Wang, Yahui;Yu, Wenbo;Wang, Zhanhui;Ding, Shuangyang;Wen, Kai;Yu, Xuezhi;Yu, Xuezhi;Li, Yuan;Ke, Yuebin;Yu, Wenbo;Wen, Kai;Wang, Zhanhui;Li, PeiPei;Wang, Yahui;Wang, Yahui;Ding, Shuangyang

作者机构:

关键词: Chemiluminescent Enzyme Immunoassays; Diclazuril; Biotin-Streptavidin System; Chicken

期刊名称: FOOD AND AGRICULTURAL IMMUNOLOGY

ISSN: 0954-0105

年卷期: 2020 年 31 卷 1 期

页码:

摘要: A simple, sensitive and specific chemiluminescence enzyme immunoassay based on biotin-streptavidin system (BASA-CLEIA) for diclazuril was established, which was about 2 and 4 times more sensitive than the traditional chemiluminescence enzyme immunoassay (CLEIA) and enzyme-linked immunosorbent assay. In the optimized generic assay, the half maximal inhibitory concentration (IC50) of the BASA-CLEIA for diclazuril was 0.48 mu g/kg. The limit of detection for diclazuril was 0.02 mu g/kg. This method showed no cross reactivity (<0.01%) with toltrazuril, robenidine hydrochloride, nicarbazin, halofuginone, amprolium, monensin, and maduramycin. In chicken muscles, the average recoveries ranged from 90.3% to 120.4%. A further comparison between the developed BSAS-CLEIA and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for real samples showed good correlation (p < 0.01). The results indicated that the simple, sensitive and specific BASA-CLEIA method could be used for the analysis of diclazuril in real samples.

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