数字农科院2.0

A Preliminary Study On Drgs And Spinal Cord Of A Galanin Receptor 2-Egfp Transgenic Mouse

文献类型： 外文期刊

作者： Lyu, C; Xia, S; Lyu, GW; Dun, XP; Zheng, K; Su, J; Barde, S; Xu, ZQD; Hokfelt, T; Shi, TJS

作者机构：

关键词： Autoreceptor; Coexistence; G Protein-Coupled Receptor; Nerve Injury; Neuropeptides; Pain

期刊名称： NEUROPEPTIDES

ISSN： 0143-4179

年卷期： 2020 年 79 卷

页码：

收录情况： JCR（2021版）

摘要： The neuropeptide galanin functions via three G-protein coupled receptors, Gal(1-3)-R. Both Gal(1)-R and (2)-R are involved in pain signaling at the spinal level. Here a Gal(2)-R-EGFP transgenic (TG) mouse was generated and studied in pain tests and by characterizing Gal(2)-R expression in both sensory ganglia and spinal cord. After peripheral spared nerve injury, mechanical allodynia developed and was ipsilaterally similar between wild type (WT) and TG mice. A Gal(2)-R-EGFP-positive signal was primarily observed in small and medium-sized dorsal root ganglion (DRG) neurons and in spinal interneurons and processes. No significant difference in size distribution of DRG neuronal profiles was found between TG and WT mice. Both percentage and fluorescence intensity of Gal(2)-R-EGFP-positive neuronal profiles were overall significantly upregulated in ipsilateral DRGs as compared to contralateral DRGs. There was an ipsilateral reduction in substance P-positive and calcitonin gene-related peptide (CGRP)-positive neuronal profiles, and this reduction was more pronounced in TG as compared to WT mice. Moreover, Gal(2)-R-EGFP partly co-localized with three pain-related neuropeptides, CGRP, neuropeptide Y and galanin, both in intact and injured DRGs, and with galanin also in local neurons in the superficial dorsal horn. Taken together, the present results provide novel information on the localization and phenotype of DRG and spinal neurons expressing the second galanin receptor, Gal(2)-R, and on phenotypic changes following peripheral nerve injury. Gal(2)-R may also be involved in autoreceptor signaling.

分类号：

  • 相关文献

[1]Neuronal Plasticity Of Trigeminal Ganglia I.n Mice Following Nerve I njury. Lynds, R, Lyu, C, Lyu, GW, Shi, XQ, Rosen, A, Mustafa, K, Shi, TJS. 2017

[2]Isolation Functional Characterization Of Allatotropin Receptor From The Cotton Bollworm, Helicoverpa Armigera. Zhang, Jian-Guo,Hu, Fei,Thakur, Kiran,Wang, Jun,An, Shi-Hen,Zhang, Fang,Jiang, Hongbo,Jiang, Xing-Fu,Jiang, Li,Wei, Zhao-Jun. 2019

[3]Molecular Characterization Of Ecdysis Triggering H.ormone And Its Receptor I n Citrus Red Mite (Panonychus Citri). Sun, Qin-Zhe,Wang, Jin-Jun,Zhu, Lin,Jiang, Hong-Bo,Sun, Qin-Zhe,Jiang, Hong-Bo,Smagghe, Guy,Zhu, Lin,Li, Gang,Li, Gang,Zhang, Wei,Wang, Jin-Jun,Smagghe, Guy,Smagghe, Guy,Zhang, Wei. 2019

[4]Identification and characterization of adipokinetic hormone and its receptor in bumble bee queens, Bombus lantschouensis. Huipeng Yang,Jiaxing Huang,Jilian Li,Jie Wu. 2021

作者其他论文 更多>>