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G Protein-Gated Inwardly Rectifying Potassium Channel Subunit 3 Is Upregulated In Rat Drgs And Spinal Cord After Peripheral Nerve Injury

文献类型： 外文期刊

作者： Lyu, C; Lyu, GW; Mulder, J; Martinez, A; Shi, TJS

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关键词： Girk Channels; Kir3; Axotomy; Drgs; Spinal Cord; Chronic Pain

期刊名称： JOURNAL OF PAIN RESEARCH

ISSN： 1178-7090

年卷期： 2020 年 13 卷

页码：

收录情况： JCR（2021版）

摘要： Background: G protein-gated inwardly rectifying potassium (GIRK) channels are involved in the regulation of neuronal excitability. Four GIRK subunits (GIRK1-4) are expressed in rat dorsal root ganglia (DRGs). Recently, we have characterized the expression of GIRK1 and -2, and both are downregulated in rat DRGs and spinal cord after a complete sciatic nerve transection (axotomy). Here, we aimed to study the neurochemical characteristics of GIRK3, and its regulation in rat DRGs and spinal cord induced by nerve injury. Methods: A sciatic nerve axotomy was performed to study the influences of injury on GIRK3 expression in DRGs and spinal cord. A dorsal root rhizotomy and a sciatic nerve crush were employed to study the axonal transport of GIRK3 protein, respectively. Immunohistochemistry analysis was employed for investigating the neurochemical characteristics of GIRK3. Results: In control DRGs, similar to 18% of neuron profiles (NPs) were GIRK3-positive ((+)), and similar to 41%, similar to 48% and similar to 45% of GIRK3(+) NPs were CGRP(+), IB4(+) and NF200(+), respectively. GIRK3-like immunoreactivity was observed in glabrous skin of hind paws and axons originating from DRG neurons. Fourteen days after axotomy, more than one-third of DRG NPs were GIRK3(+), and among these similar to 51% and similar to 56% coexpressed galanin and neuropeptide Y, respectively. In control animals, a small group of interneurons found in the dorsal horn was GIRK3(+). In addition, GIRK3(+) processes could be observed in superficial laminae of spinal dorsal horn. After nerve injury, the intensity of GIRK3-like immunoreactivity in the superficial layers was increased. Evidence based on rhizotomy and sciatic nerve crush indicated both anterograde and retrograde transport of GIRK3. Conclusion: Our study demonstrates that GIRK3 is expressed in sensory neurons and spinal cord. GIRK3 has both anterograde and retrograde axonal transport. GIRK3 expression can be regulated by peripheral nerve injury.

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[1]Effect Of Nerve Injury On T.he Number Of Dorsal R oot Ganglion Neurons And Autotomy Behavior In Adult Bax-Deficient Mice. Lyu, C, Lyu, GW, Martinez, A, Shi, TJS. 2017

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