数字农科院2.0

Deletion Of Pseudorabies Virus Us2 G.ene Enhances Viral Titers I n A Porcine Cerebral Cortex Primary Culture System

文献类型： 外文期刊

作者： Lyu, C; Wang, SW; Sun, MX; Tang, YD; Peng, JM; Tian, ZJ; Cai, XH

作者机构：

关键词： Pseudorabies virus; Genotype II; Varicosities; US2; Neuronal pathogenicity

期刊名称： VIRUS GENES

ISSN： 0920-8569

年卷期： 2018 年 54 卷 3 期

页码：

收录情况： JCR（2021版）

摘要： Pseudorabies virus (PRV) is a neurotropic virus with the ability to infect peripheral sensory ganglia. The transport of PRV from the peripheral to the central nervous system can cause lethal encephalitis in young piglets. However, the pathogenicity of PRV in the cerebral cortex remains poorly understood. In the present study, we developed a porcine cerebral cortex primary culture system (PCCS) using cerebral cortex tissue dissected from a 3-day-old piglet to investigate the pathogenicity of wild-type (WT) and US2 deleted (Delta US2) PRV in the CNS in vitro. Immunofluorescence assays revealed cell bodies and neurites as the cellular locations infected by PRV. Growth kinetic analysis showed a persistent increase in WT and Delta US2 viral titers in PCCS from 4 to 24 h post infection (hpi), thus indicating that US2 deletion did not disrupt viral growth. However, the mean plaque size was significantly higher in Delta US2 PRV than in WT PRV in infected Vero cells. The viral titers and DNA levels of Delta US2 PRV were significantly higher at 8 hpi than at 4 hpi, whereas those of WT showed no significant difference between the two time points in PCCS. Morphological investigation revealed induction of massive amounts of bouton-like swellings (varicosities) along the axon shaft in both WT and Delta US2 PRV-infected neurons in the PCCS. Our data suggest that PRV US2 gene deletion enhances viral titers in PCCS but does not affect the varicosities induced by the viral infection.

分类号：

  • 相关文献

[1]Highly Efficient CRISPR/Cas9-Mediated Homologous Recombination Promotes the Rapid Generation of Bacterial Artificial Chromosomes of Pseudorabies Virus. Guo, Jin-Chao,Tang, Yan-Dong,Zhao, Kuan,Wang, Tong-Yun,Liu, Ji-Ting,Gao, Jia-Cong,Chang, Xiao-Bo,Cui, Hong-Yu,Tian, Zhi-Jun,Can, Xue-Hui,An, Tong-Qing. 2016

[2]Emergence of a Pseudorabies virus variant with increased virulence to piglets. Tong, Wu,Liu, Fei,Zheng, Hao,Liang, Chao,Zhou, Yan-jun,Jiang, Yi-feng,Shan, Tong-ling,Gao, Fei,Li, Guo-xin,Tong, Guang-zhi,Tong, Wu,Zheng, Hao,Li, Guo-xin,Tong, Guang-zhi.

[3]Recombinant Pseudorabies Virus (PRV) Expressing Firefly Luciferase Effectively Screened for CRISPR/Cas9 Single Guide RNAs and Antiviral Compounds. Tang, Yan-Dong,Liu, Ji-Ting,Fang, Qiong-Qiong,Wang, Tong-Yun,Sun, Ming-Xia,An, Tong-Qing,Tian, Zhi-Jun,Cai, Xue-Hui,Liu, Ji-Ting. 2016

[4]A live, attenuated pseudorabies virus strain JS-2012 deleted for gE/gI protects against both classical and emerging strains. Tong, Wu,Li, Guoxin,Liang, Chao,Liu, Fei,Tian, Qing,Cao, Yanyun,Li, Lin,Zheng, Xuchen,Zheng, Hao,Tong, Guangzhi,Tong, Wu,Li, Guoxin,Tong, Guangzhi.

[5]Genomic analyses reveal that partial sequence of an earlier pseudorabies virus in China is originated from a Bartha-vaccine-like strain. Ye, Chao,Guo, Jin-Chao,Gao, Jia-Cong,Wang, Tong-Yun,Zhao, Kuan,Chang, Xiao-Bo,Wang, Qian,Peng, Jin-Mei,Tian, Zhi-Jun,Cai, Xue-Hui,An, Tong-Qing,Ye, Chao,Tong, Guang-Zhi.

[6]Expression of bioactive porcine interferon-alpha in Lactobacillus casei. Ma, Shi-jie,Li, Xin-Sheng,Guo, Xiao-Qing,Fu, Peng-Fei,Yang, Ming-Fan,Chen, Hong-Ying,Li, Kun.

[7]Pseudorabies viral replication is inhibited by a novel target of miR-21. Huang, Jing,Fu, Liangliang,Jia, Hao,Zhu, Mengjin,Li, Xinyun,Zhao, Shuhong,Huang, Jing,Ma, Guojian.

[8]Pathogenicity and immunogenicity of a gE/gI/TK gene-deleted pseudorabies virus variant in susceptible animals. Cong, Xin,Lei, Jian-Lin,Xia, Shui-Li,Wang, Yi-Min,Li, Yongfeng,Li, Su,Luo, Yuzi,Sun, Yuan,Qiu, Hua-Ji.

[9]The pseudorabies virus DNA polymerase processivity factor UL42 exists as a monomer in vitro and in vivo. Wang, Yi-Ping,Huang, Li-Ping,Du, Wen-Juan,Wei, Yan-Wu,Xia, De-Li,Wu, Hong-Li,Feng, Li,Liu, Chang-Ming.

[10]A novel gE-deleted pseudorabies virus (PRV) provides rapid and complete protection from lethal challenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in China. Wang, Chun-Hua,Yuan, Jin,Qin, Hua-Yang,Luo, Yuzi,Cong, Xin,Li, Yongfeng,Chen, Jianing,Li, Su,Sun, Yuan,Qiu, Hua-Ji.

[11]Attenuated live vaccine (Bartha-K16) caused pseudorabies (Aujeszky's disease) in sheep. Kong, Hanjin,Zhang, Keshan,Liu, Yongjie,Shang, Youjun,Liu, Xiangtao,Kong, Hanjin,Wu, Bin,Liu, Xiangtao.

[12]The Pseudorabies Virus DNA Polymerase Accessory Subunit UL42 Directs Nuclear Transport of the Holoenzyme. Wang, Yi-Ping,Du, Wen-Juan,Huang, Li-Ping,Wei, Yan-Wu,Wu, Hong-Li,Feng, Li,Liu, Chang-Ming. 2016

[13]Assembly of pseudorabies virus genome-based transfer vehicle carrying major antigen sites of S gene of transmissible gastroenteritis virus: Potential perspective for developing live vector vaccines. Yin, Jiechao,Ren, Xiaofeng,Tian, Zhijun,Li, Yijing. 2007

[14]Targeting the pseudorabies virus DNA polymerase processivity factor UL42 by RNA interference efficiently inhibits viral replication. Wang, Yi-Ping,Huang, Li-Ping,Du, Wen Juan,Wei, Yan-Wu,Wu, Hong-Li,Feng, Li,Liu, Chang-Ming.

[15]A recombinant pseudorabies virus encoding the HA gene from H3N2 subtype swine influenza virus protects mice from virulent challenge. Tian, ZJ,Zhou, GH,Zheng, BL,Qiu, HJ,Ni, JQ,Yang, HL,Yin, XN,Hu, SP,Tong, GZ. 2006

[16]Characterization of monoclonal antibodies that recognize the amino- and carboxy-terminal epitopes of the pseudorabies virus UL42 protein. Du, Wenjuan,Wang, Yiping,Huang, Liping,Wei, Yanwu,Chen, Dongjie,Sun, Jianhui,Wu, Hongli,Feng, Li,Liu, Changming.

[17]Live attenuated pseudorabies virus developed using the CRISPR/Cas9 system. Tang, Yan-Dong,Liu, Ji-Ting,Wang, Tong-Yun,An, Tong-Qing,Sun, Ming-Xia,Wang, Shu-Jie,Fang, Qiong-Qiong,Hou, Lin-lin,Tian, Zhi-Jun,Cai, Xue-Hui,Liu, Ji-Ting.

[18]A high-temperature passaging attenuated Pseudorabies vaccine protects piglets completely. against emerging PRV variant. Liang, Chao,Zhou, En-min,Liang, Chao,Tong, Wu,Zheng, Hao,Liu, Fei,Wu, Jiqiang,Li, Guoxin,Tong, Guangzhi,Tong, Wu,Zheng, Hao,Li, Guoxin,Tong, Guangzhi.

[19]A High-Temperature Passaging Attenuated Pseudorabies V.accine Protects Piglets Completely. A gainst Emerging Prv Variant. Liang, C, Tong, W, Zheng, H, Liu, F, Wu, JQ, Li, GX, Zhou, EM, Tong, GZ. 2017

[20]Generation And Immunogenicity Of A Recombinant Pseudorabies Virus Co-Expressing Classical Swine Fever Virus E2 Protein And Porcine Circovirus Type 2 Capsid Protein Based On Fosmid Library Platform. Abid, M, Teklue, T, Li, YF, Wu, HX, Wang, T, Qiu, HJ, Sun, Y. 2019

作者其他论文 更多>>