数字农科院2.0

Cry78Ba1, One Novel Crystal Protein From Bacillus Thuringiensis With High Insecticidal Activity Against Rice Planthopper

文献类型： 外文期刊

作者： Cao, BB; Shu, CL; Geng, LL; Song, FP; Zhang, J

作者机构：

关键词： Cry78Ba1; Rice Planthopper; Bacillus Thuringiensis; Insecticidal Activity; Food Safety Assessment

期刊名称： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN： 0021-8561

年卷期： 2020 年 68 卷 8 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： The rice planthopper is a very important hemipteran pest that preys on rice and substantially affects the safety of rice production. Moreover, the long-term prevention and control of these pests with chemical pesticides has led to an increase in the resistance of the rice planthopper as well as serious environmental pollution and food safety problems. Bacillus thuringiensis (Bt) has been used for the efficient and green control of a variety of rice pests. Therefore, based on the high-throughput screening of Bt strains that are active against the rice planthopper, we found that Bt strain B4F11 showed certain insecticidal activity against Laodelphax striatellus Fallen, and we have identified a novel insecticidal protein Cry78Ba1 from the Bt strain B4F11, which is expected to provide the specific and safe control of the rice planthopper. The Cry78Ba1 protein is composed of 380 amino acid residues with a molecular weight of 42.55 kDa and contains conserved Ricin_B_Lectin and Toxin_10 superfamily domains. It displays high insecticidal activity against L. striatellus with a lethal concentration (LC50) of 9.723 mu g/mL. More importantly, this Toxin_10-like protein does not display sequence homology to any known allergen and can be degraded and inactivated rapidly when heated at 90 degrees C and in simulated gastrointestinal fluid. In summary, Cry78Ba1 has great potential for applications in the efficient and safe prevention and control of the rice planthopper.

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