数字农科院2.0

A Bacillus Thuringiensis Chitin-Binding Protein Is Involved In Insect Peritrophic Matrix Adhesion And Takes Part In The Infection Process

文献类型： 外文期刊

作者： Qin, JX; Tong, ZX; Zhan, YL; Buisson, C; Song, FP; He, KL; Nielsen-LeRoux, C; Guo, SY

作者机构：

关键词： Bacillus Thuringiensis; Chitin-Binding Protein; Adhesion; Peritrophic Matrix

期刊名称： TOXINS

ISSN： 2072-6651

年卷期： 2020 年 12 卷 4 期

页码：

收录情况： JCR（2021版）

摘要： Bacillus thuringiensis (Bt) is used for insect pest control, and its larvicidal activity is primarily attributed to Cry toxins. Other factors participate in infection, and limited information is available regarding factors acting on the peritrophic matrix (PM). This study aimed to investigate the role of a Bt chitin-binding protein (CBPA) that had been previously shown to be expressed at pH 9 in vitro and could therefore be expressed in the alkaline gut of lepidopteron larvae. A increment cbpA mutant was generated that was 10-fold less virulent than wild-type Bt HD73 towards Ostrinia furnacalis neonate larvae, indicating its important role in infection. Purified recombinant Escherichia coli CBPA was shown to have a chitin affinity, thus indicating a possible interaction with the chitin-rich PM. A translational GFP-CBPA fusion elucidated the localization of CBPA on the bacterial surface, and the transcriptional activity of the promoter PcbpA was immediately induced and confirmed at pH 9. Next, in order to connect surface expression and possible in vivo gut activity, last instar Galleria mellonella (Gm) larvae (not susceptible to Bt HD-73) were used as a model to follow CBPA in gut expression, bacterial transit, and PM adhesion. CBPA-GFP was quickly expressed in the Gm gut lumen, and more Bt HD73 strain bacteria adhered to the PM than those of the increment cbpA mutant strain. Therefore, CBPA may help to retain the bacteria, via the PM binding, close to the gut surface and thus takes part in the early steps of Bt gut interactions.

分类号：

  • 相关文献

[1]Resistance alleviation in the larval cotton bollworm to fenvalerate after pretreatment with bacillus thuringiensis. 谭维嘉,梁革梅,郭予元. 1999

[2]利用ubi1内含子增强Bt cry1Ah基因在转基因玉米中的表达. 郎志宏,张杰,何康来,宋福平,黄大昉. 2008

[3]棉铃虫中肠氨肽酶APN4与Cry1Ac、Cry2Aa结合能力的比较. 葛朝虹,肖玉涛,梁革梅. 2015

[4]产Zwittermicin A的苏云金芽孢杆菌菌株的筛选及抗性基因zmaR的克隆与表达. 宋福平,李卓夫,张杰,姜声华,刘大群,黄大昉. 2005

[5]Pdh Modulate Virulence Through Reducing S.tress Tolerance And Biofilm F ormation Of Streptococcus Suis Serotype 2. Wang, Y, Wang, YX, Liu, BB, Wang, SH, Li, JP, Gong, SL, Sun, LY, Yi, L. 2019

[6]Comparative Proteomics Of Peritrophic Matrix P.rovides An Insight Into I ts Role In Cry1Ac Resistance Of Cotton Bollworm Helicoverpa Armigera. Chakrabarty, Swapan,Jin, Minghui,Jin, Minghui,Liao, Chongyu,Wu, Kongming,Xiao, Yutao. 2019

[7]The Cadherin Protein Is Not Involved In Susceptibility Tobacillus Thuringiensiscry1Ab Or Cry1Fa Toxins Inspodoptera Frugiperda. Gomez, Isabel,Yang, Yanchao,Yang, Yongbo,Liu, Kaiyu,Liu, Leilei,Zhang, Jianfeng,Jin, Minghui,Bravo, Alejandra,Xiao, Yutao,Soberon, Mario. 2020

[8]Bt Proteins Have No Detrimental E.ffects On Larvae Of T he Green Lacewing, Chrysopa Pallens (Rambur) (Neuroptera: Chrysopidae). Muhammad, M. S.,Zhang, S.,Ali, I.,Iqbal, M.,Ali, I.,Ali, I.,Cui, J. J.,Ali, I.. 2018

[9]PROTEOMIC ANALYSIS OF NOVEL Cry1Ac BINDING PROTEINS IN Helicoverpa armigera (HUBNER). Chen, Li-Zhen,Liang, Ge-Mei,Zhang, Jie,Wu, Kong-Ming,Guo, Yu-Yuan,Rector, Brian G.. 2010

[10]Feeding behaviour of Helicoverpa armigera larvae on insect-resistant transgenic cotton and non-transgenic cotton. Zhang, JH,Wang, CZ,Qin, JD,Guo, SD. 2004

[11]Processing of delta-endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1 in Heliothis armigera midgut juice and the effects of protease inhibitors. Shao, ZZ,Cui, YL,Liu, XL,Yi, HQ,Ji, JH,Yu, ZN. 1998

[12]Bt Toxin Cry1Ie Causes No Negative Effects on Survival, Pollen Consumption, or Olfactory Learning in Worker Honey Bees (Hymenoptera: Apidae). Diao, Qing-Yun,Jia, Hui-Ru,Geng, Li-Li.

[13]Reproductive Cost Associated With Juvenile Hormone in Bt-Resistant Strains of Helicoverpa armigera (Lepidoptera: Noctuidae). Wang, B. J.,Chen, L.,Khaing, M. M.,Lu, Y. H.,Liang, G. M.,Guo, Y. Y.,Ma, L..

[14]Penetration of a Single Domain of Bacillus thuringiensis Cry1Ie-Domain I to a Lipid Membrane In vitro. Li Jie,Chen Zhen,He Kang-lai. 2014

[15]Identification of vip3A-type genes from Bacillus thuringiensis strains and characterization of a novel vip3A-type gene. Song, F.,Zhang, J.,Liu, R.,He, K.,Tan, J.,Huang, D.. 2007

[16]Impacts of transgenic cry1Ab rice on non-target planthoppers and their main predator Cyrtorhinus lividipennis (Hemiptera : Miridae) - A case study of the compatibility of Bt rice with biological control. Liu, Zhi-Cheng,Ye, Gong-yin,Shen, Zhi-cheng,Hu, Cui,Peng, Yu-fa,Altosaar, Illimar,Shelton, Anthony M.. 2007

[17]Development of Bt Rice and Bt Maize in China and Their Efficacy in Target Pest Control. Liu, Qingsong,Peng, Yufa,Li, Yunhe,Hallerman, Eric. 2016

[18]Toxicity and binding analyses of Bacillus thuringiensis toxin Vip3A in Cry1Ac-resistant and -susceptible strains of Helicoverpa armigera (Hubner). Lu Qiong,Zhang Yan,Liang Ge-mei,Zhang Qian,Chen Li-zhen. 2015

[19]Use of a pooled clone method to isolate a novel Bacillus thuringiensis Cry2A toxin with activity against Ostrinia furnacalis. Shu, Changlong,Zhang, Jingtao,Chen, Guihua,Liang, Gemei,He, Kanglai,Huang, Dafang,Zhang, Jie,Song, Fuping,Shu, Changlong,Zhang, Jie,Zhang, Jingtao,Huang, Dafang,Crickmore, Neil. 2013

[20]Transgenic cotton co-expressing chimeric Vip3AcAa and Cry1Ac confers effective protection against Cry1Ac-resistant cotton bollworm. Chen, Wen-bo,Liu, Chen-xi,Xiao, Yu-tao,Wu, Kong-ming,Lu, Guo-qing,Cheng, Hong-mei,Xu, Chao,Shen, Zhi-cheng,Soberon, Mario,Bravo, Alejandra.

作者其他论文 更多>>