数字农科院2.0

Expression Profiles Of The Trehalose-6-Phosphate S.ynthase Gene Associated With T hermal Stress In Ostrinia Furnacalis (Lepidoptera: Crambidae)

文献类型： 外文期刊

作者： Jin, TT; Gao, YL; He, KL; Ge, F

作者机构：

关键词： alternative splicing isoform; transcriptional analysis; heterologous expression

期刊名称： JOURNAL OF INSECT SCIENCE

ISSN： 1536-2442

年卷期： 2018 年 18 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Trehalose is the major blood sugar in insects. Physiological significance of this compound has been extensively reported. Trehalose-6-phosphate synthase (TPS) is an important enzyme in the trehalose biosynthesis pathway. Full-length cDNAs of TPS (Oftps) and its alternative splicing isoform (Oftps_isoformI) were cloned from the Asian corn borer (ACB), Ostrinia furnacalis (Guenee; Lepidoptera: Crambidae) larvae. The Of tps and Oftps_isoformI transcripts were 2913 and 1689 bp long, contained 2529 and 1293 bp open reading frames encoding proteins of 842 and 430 amino acids with a molecular mass of 94.4 and 48.6 kDa, respectively. Transcriptional profiling and response to thermal stress of Of tps gene were determined by quantitative real-time PCR showing that the Of tps was predominantly expressed in the larval fat body, significantly enhanced during molting and transformation; and thermal stress also induced Of tps expression. Gene structure analysis is indicating that one TPS domain and one trehalose-6-phosphate phosphatase (TPP) domain were located at the N- and C-termini of Of TPS, respectively, while only the TPS domain was detected in OfTPS_isoformI. Three-dimensional modeling and heterologous expression were developed to predict the putative functions of OfTPS and OfTPS_isoformI. We infer that the expression of Of tps gene is thermally induced and might be crucial for larvae survival.

分类号：

  • 相关文献

[1]Characterization Of A Rice Dwarf A.nd Narrow Leaf 2 M utant. Adedze, YMN, Wei, XJ, Sheng, ZH, Jiao, GA, Tang, SQ, Hu, PS. 2017

[2]A Putative R3 Myb Repressor I.s The Candidate Gene U nderlying Atroviolacium, A Locus For Anthocyanin Pigmentation In Tomato Fruit. Cao, X, Qiu, ZK, Wang, XT, Van Giang, T, Liu, XL, Wang, J, Wang, XX, Gao, JC, Guo, YM, Du, YC, Wang, GP, Huang, ZJ. 2017

[3]Transcriptional Response Of Subcutaneous White A.dipose Tissue To Acute C old Exposure In Mice. Liang, XJ, Pan, JF, Cao, CW, Zhang, LL, Zhao, Y, Fan, YP, Li, K, Tao, C, Wang, YF. 2019

[4]Nadph-Cytochrome P450 Reductase Mediates The R.esistance Of Aphis (Toxoptera) C itricidus (Kirkaldy) To Abamectin. Ding, Bi-Yue,Jing, Tian-Xing,Jing, Tian-Xing,Wei, Dan-Dan,Ding, Bi-Yue,Tan, Yang,Wei, Dan-Dan,Wang, Jin-Jun,Wang, Jin-Jun,Dou, Wei,Dou, Wei,Tan, Yang. 2018

[5]Functional Characterization Of Bdb1, A W.ell-Conserved Carboxylesterase Among Tephritid F ruit Flies Associated With Malathion Resistance In Bactrocera Dorsalis (Hendel). Wang, Luo-Luo,Meng, Li-Wei,Wang, Luo-Luo,Wang, Jin-Jun,Wang, Jin-Jun,Lu, Xue-Ping,Smagghe, Guy,Smagghe, Guy,Smagghe, Guy,Lu, Xue-Ping,Meng, Li-Wei. 2017

[6]Identification And Functional Characterization Of A.n Odorant Receptor In P ea Aphid, Acyrthosiphon Pisum. Wang, Gui-Rong,Liu, Yang,Yan, Shan-Chun,Zhang, Rui-Bin,Zhang, Rui-Bin. 2019

[7]Two Acidic, Thermophilic Gh28 Polygalacturonases F.rom Talaromyces Leycettanus Jcm 1 2802 With Application Potentials For Grape Juice Clarification. Li, YQ, Wang, Y, Tu, T, Zhang, DD, Ma, R, You, S, Wang, XY, Yao, B, Luo, HY, Xu, B. 2017

[8]Identification Of A Novel Type I Pullulanase From Fervidobacterium Nodosum Rt17-B1, With High Thermostability And Suitable Optimal Ph. Yang, Y, Zhu, YY, Obaroakpo, JU, Zhang, SW, Lu, J, Yang, L, Ni, DW, Pang, XY, Lv, JP. 2020

[9]Analysis Of Bnmtl, A Novel M.etallothionein-Like Protein In The B ast Fiber Crop Ramie (Boehmeria Nivea). Gao, G, Chen, JK, Chen, P, Chen, KM, Abubakar, AS, Yu, CM, Zhu, AG. 2019

[10]An Alkaline And Surfactant-Tolerant Lipase F.rom Trichoderma Lentiforme Accc30425 W ith High Application Potential In The Detergent Industry. Wang, YZ, Ma, R, Li, SG, Gong, MB, Yao, B, Bai, YG, Gu, JG. 2018

[11]Enhanced Heterologous Expression Of Trichoderma R.eesei Cel5A/Cel6A In Pichia P astoris With Extracellular Co-Expression Of Vitreoscilla Hemoglobin. Sun, FF, Yang, HM, Bai, RH, Fang, X, Wang, F, He, J, Tu, MB. 2018

[12]Harnessing The Knowledge Of Protein S.ecretion For Enhanced Protein P roduction In Filamentous Fungi. Sun, XH, Su, XY. 2019

[13]Molecular Characterization And Phylogenetic Analysis O.f One Omega-Gliadin Gene F rom Aegilops Speltoides L.. Wang, WQ, Wang, K, Chen, X, Prodanovic, S, Li, XH, Ye, XG, Yan, YM. 2018

[14]Heterologous expression of Arabidopsis thaliana rty gene in strawberry (Fragaria × ananassa Duch.) improves drought tolerance. Maofu Li,Yuan Yang,Ali Raza,Shanshan Yin,Hua Wang,Yuntao Zhang,Jing Dong,Guixia Wang,Chuanfei Zhong,Hong Zhang,Jiashen Liu,Wanmei Jin. 2021

[15]Production Pectin Oligosaccharides Using Humicola Insolens Y1-Derived Unusual Pectate Lyase. Wang, ZY, Xu, B, Luo, HY, Meng, K, Wang, Y, Liu, MT, Bai, YG, Yao, B, Tu, T. 2020

作者其他论文 更多>>