数字农科院2.0

Fatty Acid Biosynthesis And Transcriptional Regulation Of Stearoyl-Coa Desaturase 1 (Scd1) In Buffalo Milk

文献类型： 外文期刊

作者： Rehman, Saif Ur;Shafique, Laiba;Luo, Chan;Liu, Qingyou;Wang, Zhiqiang;Cui, Kuiqing;Qian, Qian;Ruan, Jue;Liu, Qingyou;Lu, Suyu;Li, Zhipeng

作者机构：

关键词： Scd1; Buffalo Milk; Fatty Acid; Rna Interference; Overexpression

期刊名称： BMC GENETICS

ISSN： 1471-2156

年卷期： 2020 年 21 卷 1 期

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摘要： Background Buffalo milk is considered as a highly nutritious food owing to its higher contents of fatty acids (FA) and rich nutrient profile. Higher fat contents of buffalo milk make it suitable for processing to develop various healthy and nutritious products. Moreover, buffalo milk contains more unsaturated FAs (UFA) such as oleic and linolenic acid, which are important from the human health point of view owing to their desirable physiological effects. However, inadequate information is available about the chemical composition and mechanism of FA synthesis in buffalo milk. In this study, we hypothesized that expression of SCD1 gene could alter the biosynthesis of FA in epithelial cells of mammary gland and subsequently affect the FA contents in buffalo milk. We investigated the transcriptional and biological role of Stearoyl-CoA Desaturase 1 (SCD1) in the buffalo mammary epithelial cells (BMECs) during FA and triacylglycerol (TAG) synthesis. Results Results revealed that unsaturated fatty acid contents were much higher in concentration in buffalo milk as compared to Holstein cow. Significant increase in the expression level of FAS, ACACA, SREBP1, PPARG, GPAT, and AGPAT genes was observed in response to altered expression of SCD1 in buffalo milk. Moreover, change in SCD1 gene in BMECs also mediated the expression of genes related to FA biosynthesis subsequently leading to alter the FA composition. Overexpression of SCD1 significantly increased the expression of genes associated with FA and TAG synthesis leading to enhance FA and unsaturated FA contents in BMECs. However, down-regulation of SCD1 exhibited opposite consequences. Conclusion Our study provides mechanistic insights on transcriptional regulation of SCD1 to alter FA and TAG synthesis through directly or indirectly mediating biosynthesis and metabolic pathways in BMECs. We provide preliminary findings regarding engineering of FA contents in buffalo milk through SCD1 signaling.

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