数字农科院2.0

Optimization Of The Protoplast Transient Expression System For Gene Functional Studies In Strawberry (Fragaria Vesca)

文献类型： 外文期刊

作者： Gou, YJ; Li, YL; Bi, PP; Wang, DJ; Ma, YY; Hu, Y; Zhou, HC; Wen, YQ; Feng, JY

作者机构：

关键词： Strawberry; Protoplast Isolation; Transient Gene Expression; Crispr; Cas9; Fvtcp17

期刊名称： PLANT CELL TISSUE AND ORGAN CULTURE

ISSN： 0167-6857

年卷期： 2020 年 141 卷 1 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Key message We report a highly efficient mesophyll protoplast system for transient gene expression and induction of CRISPR/Cas9-mediated genome editing in strawberry. Polyethylene glycol (PEG)-mediated transient expression system in plant protoplasts has been widely used in a variety of plants for gene function characterization. However, such a system has not been developed for strawberry. In this study, we report a method for obtaining high quality and high yield protoplasts from strawberry leaves. In addition, we developed an efficient transient gene expression system based on the PEG-mediated method, and the system has been successfully applied to studies of protein expression, protein subcellular localization and protein-protein interaction in strawberry. Furthermore, the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9) technology was used to enable targeted mutagenesis of the FvTCP17 gene in strawberry protoplasts. Taken together, our results demonstrate the potential of this highly efficient mesophyll protoplast system for transient gene expression and induction of CRISPR/Cas9-mediated genome editing in strawberry.

分类号：

  • 相关文献

[1]Editing Of Rice Isoamylase Gene I.sa1 Provides Insights Into I ts Function In Starch Formation. Luo Ju,Jiao Guiai,Sheng Zhonghua,Cai Yicong,Wang Jianlong,Tang Shaoqing,Feng Baobing,Chao Shufen,Hu Peisong,Tang Shaoqing,Wei Xiangjin. 2019

[2]Mutagenesis Of Gmft2A And Gmft5A M.ediated By Crispr/Cas9 Contributes F or Expanding The Regional Adaptability Of Soybean. Jiang, Bingjun,Liu, Luping,Hou, Wensheng,Han, Tianfu,Chen, Li,Wu, Tingting,Yao, Weiwei,Wu, Cunxiang,Sun, Shi,Wang, Liwei,Yao, Weiwei,Cai, Yupeng,Hou, Wensheng,Cai, Yupeng,Chen, Li,Yuan, Shan. 2019

[3]Engineering Canker-Resistant Plants Through Crispr/Cas9-Targeted E.diting Of The Susceptibility G ene Cslob1 Promoter In Citrus. Wu, Liu,He, Yongrui,He, Yongrui,Yao, Lixiao,Xu, Lanzhen,Lei, Tiangang,Chen, Shanchun,Xu, Lanzhen,Zou, Xiuping,Zou, Xiuping,Peng, Aihong,Yao, Lixiao,Lei, Tiangang,Peng, Aihong,Chen, Shanchun. 2017

[4]A Palmitoyltransferase Approximated Gene Bm-App Regulates Wing Development In Bombyx Mori. Hou, Cheng-Xiang,Li, Mu-Wang,Hou, Cheng-Xiang,Wang, Tai-Chu,Sun, Fan,Li, Mu-Wang,Zhang, Zhong-Jie,Liu, Xiao-Jing,Ma, Xiao,Yu, Ye. 2020

[5]Crispr/Cas9-Mediated Editing Of Cswrky22 Reduces S.usceptibility To Xanthomonas Citri S ubsp. Citri In Wanjincheng Orange (Citrus Sinensis (L.) Osbeck). Peng, Aihong,Chen, Shanchun,Wang, Lijuan,Zou, Xiuping,He, Yongrui,Xie, Zhu,Xie, Zhu,Wang, Lijuan,Chen, Shanchun,He, Yongrui,Zou, Xiuping,Peng, Aihong. 2019

[6]Generation Of Transgenic Cloned Buffalo Embryos Harboring The Egfp Gene In The Y Chromosome Using Crispr/Cas9-Mediated Targeted Integration. Zhao, Xiuling,Pang, Chunying,Pang, Chunying,Liang, Xianwei,Liang, Xianwei,Nie, Junyu,Tang, Yuyan,He, Wengtan,Xiao, Kai,Lu, Yangqing,Zhang, Ming. 2020

[7]Fatty Acid Synthase Knockout Impairs E.arly Embryonic Development Via I nduction Of Endoplasmic Reticulum Stress In Pigs. Guo, J, Niu, YJ, Shin, KT, Kwon, JW, Kim, NH, Cui, XS. 2018

[8]Identification Of Glyceraldehyde-3-Phosphate Dehydrogenase Gene A.s An Alternative Safe H arbor Locus In Pig Genome. Han, XS, Xiong, YC, Zhao, CZ, Xie, SS, Li, CC, Li, XY, Liu, XD, Li, K, Zhao, SH, Ruan, JX. 2019

[9]Base Editing In Crops: Current A.dvances, Limitations And Future I mplications. Mishra, R, Joshi, RK, Zhao, KJ. 2019

[10]Knockout Of Two Bnamax1 Homologs B.y Crispr/Cas9-Targeted Mutagenesis Improves P lant Architecture And Increases Yield In Rapeseed (Brassica Napus L.). Zheng, M, Zhang, L, Tang, M, Liu, JL, Liu, HF, Yang, HL, Fan, SH, Terzaghi, W, Wang, HZ, Hua, W. 2019

[11]Chromosomal Deletions Mediated By Crispr/Cas9 I.n Helicoverpa Armigera. Hu, Jie,Jin, Ming-Hui,Xue, Chao-Bin,Cheng, Ying,Jin, Ming-Hui,Xiao, Yu-Tao,Xiao, Yu-Tao,Jin, Ming-Hui,Wu, Kong-Ming. 2019

[12]Applications Of The Crispr/Cas9 System F.or Rice Grain Quality I mprovement: Perspectives And Opportunities. Fiaz, S, Ahmad, S, Noor, MA, Wang, XK, Younas, A, Riaz, A, Riaz, A, Ali, F. 2019

[13]Creation Of Early Flowering Germplasm Of Soybean By Crispr/Cas9 Technology. Han, JN, Guo, BF, Guo, Y, Zhang, B, Wang, XB, Qiu, LJ. 2019

[14]Natural Variation And Crispr/Cas9-Mediated Mutation Ingmprr37Affect Photoperiodic Flowering And Contribute To Regional Adaptation Of Soybean. Wang, LW, Sun, S, Wu, TT, Liu, LP, Sun, XG, Cai, YP, Li, JC, Jia, HC, Yuan, S, Chen, L, Jiang, BJ, Wu, CX, Hou, WS, Han, TF. 2020

[15]Functional Analysis Of The Ul24 P.rotein Of Suid Herpesvirus 1. Ye, C, Chen, J, Cheng, XF, Zhou, SS, Jiang, S, Xu, JJ, Zheng, H, Tong, W, Li, GX, Tong, GZ. 2019

[16]Identification Of Candidate Hy5-Dependent And -.Independent Regulators Of Anthocyanin B iosynthesis In Tomato. Qiu, ZK, Wang, HJ, Li, DJ, Yu, BW, Hui, QL, Yan, SS, Huang, ZJ, Cui, X, Cao, BH. 2019

[17]Establishment of an Agrobacterium‐mediated genetic transformation and CRISPR/Cas9‐mediated targeted mutagenesis in Hemp (Cannabis Sativa L.). Xiaoyu Zhang,Gencheng Xu,Chaohua Cheng,Lei Lei,Jian Sun,Ying Xu,Canhui Deng,Zhigang Dai,Zemao Yang,Xiaojun Chen,Chan Liu,Qing Tang,Jianguang Su. 2021

[18]Characterization Of The Role Of Amylo-Alpha-1,6-Glucosidase Protein In The Infectivity Of Toxoplasma Gondii. Cao, XZ, Wang, JL, Elsheikha, HM, Li, TT, Sun, LX, Liang, QL, Zhang, ZW, Lin, RQ. 2019

[19]An Orphan Pheromone Receptor Affects The Mating Behavior Of Helicoverpa Armigera. Cao, S, Huang, TY, Liu, Y, Wang, GR. 2020

[20]CRISPR/Cas9 induces exon skipping that facilitates development of fragrant rice. Tang Yongchao,Abdelrahman Mohamed,Li Jinbo,Wang Fujun,Ji Zhiyuan,Qi Huaxiong,Wang Chunlian,Zhao Kaijun. 2021

作者其他论文 更多>>