数字农科院2.0

Marek'S Disease Virus As A Crispr/Cas9 Delivery System To Defend Against Avian Leukosis Virus Infection In Chickens

文献类型： 外文期刊

作者： Liu, YZ; Xu, ZK; Zhang, Y; Yu, MM; Wang, SY; Gao, YL; Liu, CJ; Zhang, YP; Gao, L; Qi, XL; Cui, HY; Pan, Q; Li, K; Wang, XM

作者机构：

关键词： Marek'S Disease Virus; Avian Leukosis Virus; Crispr/Cas9; Infection

期刊名称： VETERINARY MICROBIOLOGY

ISSN： 0378-1135

年卷期： 2020 年 242 卷

页码：

收录情况： JCR（2021版）

摘要： The CRISPR/CRISPR-associated protein 9 (Cas9) system is a powerful gene-editing tool originally discovered as an integral mediator of bacterial adaptive immunity. Recently, this technology has been explored for its potential utility in providing new and unique treatments for viral infection. Marek's disease virus (MDV) and avian leukosis virus subgroup J (ALV-J), major immunosuppressive viruses, cause significant economic losses to the chicken industry. Here, we evaluated the efficacy of using MDV as a CRISPR/Cas9-delivery system to directly target and disrupt the reverse-transcribed products of the ALV-J RNA genome during its infection cycle in vitro and in vivo. We first screened multiple potential guide RNA (gRNA) target sites in the ALV-J genome and identified several optimized targets capable of effectively disrupting the latently integrated viral genome and providing efficient defense against new infection by ALV-J in cells. The optimal single-gRNAs and Cas9-expression cassettes were inserted into the genome of an MDV vaccine strain. The results indicated that engineered MDV stably expressing ALV-J-targeting CRISPR/Cas9 efficiently resisted ALV-J challenge in host cells. These findings demonstrated the CRISPR/Cas9 system as an effective treatment strategy against ALV-J infection. Furthermore, the results highlighted the potential of MDV as an effective delivery system for CRISPR/Cas9 in chickens.

分类号：

  • 相关文献

[1]ZBED6基因对巴马香猪脾发育的作用机制分析. 王圣楠,王丹丹,田文杰,浦亚斌,潘登科,邢向阳,马月辉,蒋琳. 2021

[2]基于CRISPR/Cas9基因编辑系统构建敲除DDX5基因的猪肾细胞系. 金彩燕,吴金恩,何童童,孙世琪,郭慧琛,张小丽. 2021

[3]采用 CRISPR/Cas9 创制黄籽高抗裂角油菜杂交种. 蔡雅婷,,郝梦宇,,李一林,,温运飞,,王会,,汪文祥,,付丽,,胡琼,,成洪涛,,梅德圣. 2024

[4]MARCH2 inhibits avian leukosis virus replication by targeting gp85 for ubiquitination and degradation. Yuntong Chen,Longbo Wu,Wenrui Fan,Ying Wang,Jinglei Li,Xuefu Zhang,Zibo Zhang,Yumeng Li,Suyan Wang,Yongzhen Liu,Xiaole Qi,Yanping Zhang,Hongyu Cui,Yulu Duan,Yulong Gao. 2025

[5]Transport Via Macropinocytic Vesicles Is C.rucial For Productive Infection W ith Bombyx Mori Nucleopolyhedrovirus. Shen, Xingjia,Tang, Xudong,Huang, Jinshan,Hao, Bifang,Nan, Wenbin,Huang, Jinshan,Tang, Xudong,Hao, Bifang,Liu, Lin,Shen, Xingjia,Li, Chenya. 2019

[6]Comparative Transcriptome Profiling Of Resistant A.nd Susceptible Sugarcane Genotypes I n Response To The Airborne Pathogen Fusarium Verticillioides. Liang, Qiang,Li, Yijie,Li, Changning,Gao, Yijing,Lei, Jingchao,Song, Xiupeng,Wang, Zeping. 2019

[7]Characterization Of A Bacterial Symbiont A.saia Sp. In The W hite-Backed Planthopper, Sogatella Furcifera, And Its Effects On Host Fitness. Ali, Asad,Li, Fei,Hou, Maolin,Ali, Asad,Hou, Maolin,Hou, Maolin,Li, Fei,Hua, Hongxia. 2019

[8]Washed Microbiota Transplantation Vs. Manual Fecal Microbiota Transplantation: Clinical Findings, Animal Studies And In Vitro Screening. Zhang, Faming,Yin, Haoran,Zhang, Faming,Zhang, Faming,Wang, Huiquan,Marcella, Cicilia,Lu, Gaochen,Shen, Quan,Marcella, Cicilia,Zhao, Zhe,Chen, Yaoyao,Zhang, Ting,Cheng, Lei,Chen, Yaoyao,Zhang, Ting,Cui, Bota,Li, Pan,Cheng, Lei,Cui, Bota,Lu, Gaochen,Ji, Guozhong,Ji, Guozhong,Liu, Yafei,Li, Pan,Liu, Yafei. 2020

[9]Review Of Seneca Valley Virus: A. Call For Increased S urveillance And Research. Zhang, XL, Zhu, ZX, Yang, F, Cao, WJ, Tian, H, Zhang, KS, Zheng, HX, Liu, XT. 2018

[10]Detection And Geographic Distribution Of S.even Facultative Endosymbionts In T wo Rhopalosiphum Aphid Species. Poncelet, Nicolas,He, Kanglai,Liu, Xuewei,Wang, Zhenying,Guo, Jianqing,Guo, Jianqing,Guo, Jianqing,Francis, Frederic. 2019

[11]Label-Free Quantitative Proteomic Analysis Of D.ifferentially Expressed Membrane Proteins O f Pulmonary Alveolar Macrophages Infected With Highly Pathogenic Porcine Reproductive And Respiratory Syndrome Virus And Its Attenuated Strain. Qu, ZH, Gao, F, Li, LW, Zhang, YJ, Jiang, YF, Yu, LX, Zhou, YJ, Zheng, H, Tong, W, Li, GX, Tong, GZ. 2017

[12]Mechanism And Complex Roles Of Hsc70 In Viral Infections. Wang, Z, Li, YT, Yang, X, Zhao, J, Cheng, YN, Wang, JK. 2020

[13]Proteomic Analysis Of Fasciola Gigantica E.xcretory And Secretory Products ( Fgesps) Interacting With Buffalo Serum Of Different Infection Periods By Shotgun Lc-Ms/Ms. Huang, SY, Yue, DM, Hou, JL, Zhang, XX, Zhang, FK, Wang, CR, Zhu, XQ. 2019

[14]Recent Advances In Nucleic Acid-Based Methods For Detection Of Helminth Infections And The Perspective Of Biosensors For Future Development. Ullah, H, Qadeer, A, Rashid, M, Rashid, MI, Cheng, GF. 2020

[15]Intercellular Transmission Of Seneca Valley Virus Mediated By Exosomes. Xu, GW, Xu, SX, Shi, XJ, Shen, CC, Hao, JH, Yan, MH, Zhang, DJ, Zhu, ZX, Zhang, KS, Zheng, HX, Liu, XT. 2020

[16]Streptococcus suis Serotype 2 Infection Causes Host Immunomodulation Through Induction of Thymic Atrophy. Gottschalk, Marcelo,Duan, Guixin,Li, Ganwu,Wang, Zhenzhong,Yu, Ying,Gottschalk, Marcelo,Wang, Gang,Meng, Fandan,Lyu, Chuang,Wang, Shujie,Cai, Xuehui,He, Xijun,Yang, Yong-Bo. 2020

[17]Fiber-1, Not Fiber-2, Directly Mediates The Infection Of The Pathogenic Serotype 4 Fowl Adenovirus Via Its Shaft And Knob Domains. Wang, WK, Liu, Q, Li, TF, Geng, TY, Chen, HJ, Xie, Q, Shao, HX, Wan, ZM, Qin, AJ, Ye, JQ. 2020

[18]Antibacterial Activity Of Polyphenols: Structure-Activity R.elationship And Influence Of H yperglycemic Condition. Xie, YX, Chen, J, Xiao, AP, Liu, LL. 2017

[19]Immune Evasion of Mycoplasma bovis. Hussam Askar,Shengli Chen,Huafang Hao,Xinmin Yan,Lina Ma,Yongsheng Liu,Yuefeng Chu. 2021

[20]Immunostimulatory Efficacy And Protective Potential Of Putative Tgerk7 Protein In Mice Experimentally Infected By Toxoplasma Gondii. Li, ZY, Guo, HT, Calderon-Mantilla, G, He, JJ, Wang, JL, Bonev, BB, Zhu, XQ, Elsheikha, HM. 2020

作者其他论文 更多>>