数字农科院2.0

Ultrasound-Assisted One-Phase Solvent Extraction Coupled With Liquid Chromatography-Quadrupole Time-Of-Flight Mass Spectrometry For Efficient Profiling Of Egg Yolk Lipids

文献类型： 外文期刊

作者： Xie, Y; Wu, BF; Wu, ZY; Tu, XH; Xu, SL; Lv, X; Yin, HQ; Xiang, JQ; Chen, H; Wei, F

作者机构：

关键词： Ultrasonic Pretreatment; One-Phase Lipid Extraction; Untargeted Lipidomics; Egg Yolk; Lc-Esi-Qtof-Ms

期刊名称： FOOD CHEMISTRY

ISSN： 0308-8146

年卷期： 2020 年 319 卷

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： In this research, a novel ultrasound-assisted extraction (USAE) method using a one-phase solvent (isopropanol, IPA) combined with liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QToF-MS) was developed for exhaustive profiling of egg yolk lipids, which are considered to be essential nutrients that have important functions for human health. Compared with IPA-vortex extraction method, the optimized IPA-USAE method shows better performance in terms of extraction time, lipid losses, lipid recoveries and the number of lipids identified. With this established simple, high throughput and reliable analytical approach, untargeted comprehensive lipid profiling of four kinds of egg yolks was achieved, thus providing a powerful tool for nutritional research of egg lipids. Considering that the lipids contained in egg yolk are quite abundant in both quantity and species, the proposed IPA-USAE combined with LC-ESI-QToF-MS is also potentially applicable to comprehensive extraction and profiling of lipids in other biological samples.

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