Study On The Antioxidant Activity And Emulsifying Properties Of Flaxseed Gum-Whey Protein Isolate Conjugates Prepared By Maillard Reaction
文献类型: 外文期刊
作者: Dong, XY; Du, SS; Deng, QC; Tang, H; Yang, C; Wei, F; Chen, H; Quek, SY; Zhou, AJ; Liu, L
作者机构:
关键词: Maillard Reaction; Conjugate; Flaxseed Gum; Whey Protein Isolate; Emulsifying Property
期刊名称: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN: 0141-8130
年卷期: 2020 年 153 卷
页码:
收录情况: JCR(2021版)
摘要: The antioxidant and emulsifying properties of flaxseed gum-whey protein isolate (FSG-WPI) conjugates prepared by Maillard reaction via controlled dry-heating were investigated. The reaction was carried out using a ratio of FSG to WPI of 1:3 at 60 degrees C and 79% relative humidity for different incubation times. The reaction was confirmed by analysis of the browning index, free amino content and soluble sulfhydryl content, as well as by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, ultraviolet-visible spectroscopy, and Fourier transform infrared spectroscopy. We found that nearly 35% of the protein participated in conjugation with FSG after 48 h of incubation. The antioxidant activity of the conjugates improved markedly after 48 and 72 h incubation time. Differential scanning calorimetry results indicated that the denaturation temperature of the conjugates increased. The FSG-WPI conjugate prepared by 72 h incubation had the best emulsifying properties in stabilizing an oil-in-water emulsion. This research provides significant knowledge for the potential applications of FSG in food industry. (C) 2019 Elsevier B.V. All rights reserved.
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