数字农科院2.0

Label-Free Comparative Proteomic Analysis Combined With Laser-Capture Microdissection Suggests Important Roles Of Stress Responses In The Black Layer Of Maize Kernels

文献类型： 外文期刊

作者： Chen, QQ; Huang, R; Xu, ZX; Zhang, YX; Li, L; Fu, JJ; Wang, GY; Wang, JH; Du, XM; Gu, RL

作者机构：

关键词： maize; black layer; seed development; proteomic analysis; stress response; MALDI-TOF MS

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN：

年卷期： 2020 年 21 卷 4 期

页码：

收录情况： JCR（2021版）

摘要： The black layer (BL) is traditionally used as an indicator for kernel harvesting in maize, as it turns visibly dark when the kernel reaches physiological maturity. However, the molecular roles of BL in kernel development have not been fully elucidated. In this work, microscopy images showed that BL began to appear at a growth stage earlier than 10 days after pollination (DAP), and its color gradually deepened to become dark as the development period progressed. Scanning electron microscopy observations revealed that BL is a tissue structure composed of several layers of cells that are gradually squeezed and compressed during kernel development. Laser-capture microdissection (LCM) was used to sample BL and its neighboring inner tissue, basal endosperm transfer layer (BETL), and outer tissue, inner epidermis (IEP), from 20 DAP of kernels. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling (MALDI-TOF MS profiling) detected 41, 104, and 120 proteins from LCM-sampled BL, BETL, and IEP, respectively. Gene ontology (GO) analysis indicated that the 41 BL proteins were primarily involved in the response to stress and stimuli. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis found that the BL proteins were enriched in several defense pathways, such as the ascorbate and aldarate metabolic pathways. Among the 41 BL proteins, six were BL-specific proteins that were only detected from BL. Annotations of five BL-specific proteins were related to stress responses. During kernel development, transcriptional expression of most BL proteins showed an increase, followed by a decrease, and reached a maximum zero to 20 DAP. These results suggest a role for BL in stress responses for protecting filial tissue against threats from maternal sides, which helps to elucidate the biological functions of BL.

分类号：

  • 相关文献

[1]Transcriptomic Analysis Of Long Non-Coding R.nas And Coding Genes U ncovers A Complex Regulatory Network That Is Involved In Maize Seed Development. Xu, Miaoyun,Wang, Lei,Zhu, Ming,Zhu, Ming,Zhang, Min,Jiang, Haiyang,Xing, Lijuan,Li, Wenzong. 2017

[2]DissectingthemaizedirectandindirectdefenseresponseagainstAsianCornBorer. 汪海,李圣彦,查象敏,朱莉,黄大昉,郎志宏. 2015

[3]TheDifferentialTranscriptionNetworkbetweenEmbryoandEndospermintheEarlyDevelopingMaizeSeed. XiaoduoLu,DijunChen,DefengShu,ZhaoZhang,WeixuanWang,ChristianKlukas,Ling-lingChen,YunliuFan,MingChen,ChunyiZhang. 2015

[4]Genetic Variation Assessment Of Stacked-Trait T.ransgenic Maize Via Conventional B reeding. Song, Yaya,Liu, Xiaojing,Wang, Zhixing,Zhang, Xin,Wang, Xujing,Yang, Jiangtao. 2019

[5]Comparative proteomic analysis reveals that the Heterosis of two maize hybrids is related to enhancement of stress response and photosynthesis respectively. Daoping Wang,Yongying Mu,Xiaojiao Hu,Bo Ma,Zhibo Wang,朱莉,Jiang Xu,Changling Huang,Yinghong Pan. 2021

[6]Proteomic Analysis Of The Function O.f A Novel Cold-Regulated M ultispanning Transmembrane Protein Cor413-Pm1 In Arabidopsis. Su, C, Chen, K, Ding, QQ, Mou, YY, Yang, R, Zhao, MJ, Ma, B, Xu, ZS, Ma, YZ, Pan, YH, Chen, M, Xi, YJ. 2018

[7]Understanding The Genetic Mechanism Of R.esistance In Aphid-Treated Alfalfa ( Medicago Sativa L.) Through Proteomic Analysis. Tu, Xiongbing,Chen, Jun,Ullah, Hidayat,Zhang, Zehua,Ullah, Hidayat. 2019

[8]Quantitative Proteomic Analysis Of Duck Embryo Fibroblasts Infected With Novel Duck Reovirus. Yang, YD, Li, L, Liu, XP, Jiang, MJ, Zhao, J, Li, XS, Zhao, C, Yi, H, Liu, SD, Li, N. 2020

[9]Itraq-Based Proteomic Analysis Of Resistant N.icotiana Tabacum In Response T o Bemisia Tabaci Infestation. Zhang, ST, Long, Y, Zhang, SJ, Li, N, Chen, DX, Jia, HF, Zhang, HY, Yang, YX. 2019

[10]Seed Development And Nutrient Accumulation A.s Affected By Light S hading In Oilseed Peony (Paeonia Ostii 'Feng Dan'). Wang Qi,Han Chen-jing,Dong He-zhong,Zhang Hong-bao. 2019

[11]Evaluation Of Seed Quality Based O.n Changes Of Internal S ubstances During Tobacco Seed (Nicotiana Tabacum L.) Development. Li, Zhan,Li, Fan,Guo, Genyuan,Gao, Yue,Pan, Ronghui,Guan, Yajing,Hu, Jin,Ma, Wenguang,Ma, Wenguang. 2018

[12]Identification Of Oil, Sugar And C.rude Fiber During Tobacco ( Nicotiana Tabacum L.) Seed Development Based On Near Infrared Spectroscopy. Hu, Jin,Niu, Yongzhi,Guan, Yajing,Zheng, Yunye,Gao, Yue,Li, Zhan,Ma, Wenguang,Ma, Wenguang,Niu, Yongzhi,Li, Cheng,Zheng, Yunye. 2018

[13]Rice FLOURY SHRUNKEN ENDOSPERM 5 Encodes a Putative Plant Organelle RNA Recognition Protein that Is Required for cis-Splicing of Mitochondrial nad4 Intron 1. Liang Wang,Wenwei Zhang,Shijia Liu,Yunlu Tian,Xi Liu,Haigang Yan,Yue Cai,Xuan Teng,Hui Dong,Rongbo Chen,Xiaokang Jiang,Yihua Wang,Jianmin Wan. 2021

[14]Proteomic Changes During Two Stages O.f Pomegranate (Punica Granatum L .) Seed Development Identified Using The Isobaric Mass Tags For Relative And Absolute Quantitation Method. Niu, J, Cao, SY, Zhang, J, Li, HX, Xue, H, Chen, LN, Zhang, FH, Zhao, DG. 2018

[15]Identification and Characterization of microRNAs in the Developing Seed of Linseed Flax (Linum usitatissimum L.). 张天豹,,裴新梧. 2020

[16]Maize Empty Pericarp602 Encodes A P.-Type Ppr Protein That I s Essential For Seed Development. Ren, ZJ, Fan, KJ, Fang, T, Zhang, JJ, Yang, L, Wang, JH, Wang, GY, Liu, YJ. 2019

[17]The Mitochondrial Pentatricopeptide Repeat Protein E.mp12 Is Involved In T he Splicing Of Three Nad2 Introns And Seed Development In Maize. Sun, Feng,Tan, Bao-Cai,Li, Xiaojie,Jiang, Ruicheng,Liu, Yiwei,Wang, Yong,Zhang, Xin,Xiu, Zhihui,Zhang, Xiaoyan,Yang, Yan-Zhuo. 2019

[18]Genome-wide identification and functional characterization of LEA genes during seed development process in linseed flax (Linum usitatissimum L.). Li Zhen,Chi Hui,Liu Caiyue,Zhang Tianbao,Han Lida,Li Liang,Pei Xinwu,Long Yan. 2021

[19]Genome-Wide Identification of lncRNAs During Rice Seed Development. Juan Zhao , Abolore Adijat Ajadi , Yifeng Wang , Xiaohong Tong , Huimei Wang , Liqun Tang , Zhiyong Li , Yazhou Shu , Xixi Liu , Shufan Li , Shuang Wang , Wanning Liu , Jian Zhang *. 2020

[20]Integrated Metabolomic and Transcriptomic Analysis of Volatile Organic Compound Biosynthesis During Mung Bean (Vigna radiata) Seed Development. Nan Xiang, Yihan Zhao, Bing Zhang, Honglin Chen, Xinbo Guo. 2025

作者其他论文 更多>>