Development And Application Of An Indirect Elisa For The Serological Detection Of Duck Tembusu Virus Infection Based On The Ns1 Protein Antigen
文献类型： 外文期刊
作者： Zhou, Q; Bi, ZL; Yin, DD; Gu, XX; Xu, ZJ; Huang, R; Xing, X; Qi, KZ; Wang, GJ
作者机构：
期刊名称： ARCHIVES OF VIROLOGY
ISSN： 0304-8608
年卷期： 2020 年 165 卷 3 期
页码：
收录情况： JCR（2021版）
摘要： Duck Tembusu virus (DTMUV) has caused significant economic losses in China since 2010. However, there is still a lack of effective methods to diagnose the disease caused by this virus, and especially to differentiate infection from vaccination. In this study, we established a novel indirect enzyme-linked immunosorbent assay (iELISA) and performed a retrospective serological survey for DTMUV in Anhui province, China. Our results show that the iELISA displayed high specificity sensitivity, and with no serological cross-reaction with other duck pathogens. These findings indicate that the newly developed iELISA could be a useful screening tool for large-scale monitoring of the epidemiology of DTMUV infection in ducks.
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