数字农科院2.0

Use Of A Modified Bacterial G.host Lysis System For T he Construction Of An Inactivated Avian Pathogenic Escherichia Coil Vaccine Candidate

文献类型： 外文期刊

作者： Hu, JG; Zuo, JK; Chen, ZG; Fu, LX; Lv, XL; Hu, SJ; Shi, XC; Jing, YW; Wang, YL; Wang, ZH; Mi, RS; Huang, Y; Liu, DH; Qi, KZ; Han, XG

作者机构：

关键词： Avian pathogenic; Escherichia coli; Lysis plasmid; Bacterial ghost; Immune protection; Gene deletion

期刊名称： VETERINARY MICROBIOLOGY

ISSN： 0378-1135

年卷期： 2019 年 229 卷

页码：

收录情况： JCR（2021版）

摘要： Vaccination is an effective strategy to prevent avian colibacillosis. Bacterial ghosts (BGs) are prepared by the controlled expression of the phiX174 gene E, which mediates the lysis of Gram-negative bacteria. Staphylococcal nuclease A may be used to produce BGs for further inactivation of host bacteria and elimination of residual genetic material. In this study, the double promoter lysis plasmid (pUC19-Delta c1857-E-rmB-A-SN) was successfully constructed and BGs were prepared at 37 degrees C. The cleavage efficiency of Escherichia coil BGs was 99.9%. Furthermore, to evaluate the immunological effects of the BG vaccines in chickens, a BG vaccine was prepared using the serotype O-2 avian pathogenic Escherichia coil deletion strain (DE17 Delta luxS Delta aroA). The results showed that the BC vaccine was able to achieve over 90% immune protection against virulent challenge using the same serotype O-2 strain (DE17 or CE35), while it showed poor cross-protection against serotypes O-1 and 078 (data not shown). The enzyme-linked immunosorbent assay results showed that the antibody levels in the immunized groups were higher than in the control group (p < 0.05), with the BG group being the highest. The cytokine tests showed that the levels of interferon-gamma in the BG immune group were higher than in the phosphate-buffered saline (PBS) control group (non-immune) (p < 0.01) and the formalin-inactivated vaccine immune group (p < 0.05), and the levels of tumor necrosis factor-alpha in the BG group were higher than in the formalin-inactivated vaccine (p > 0.05) and the PBS control groups (p < 0.05). In addition, pathological analysis revealed that the PBS control group showed typical fibrinous pericarditis and perihepatitis, whereas the immune group showed no obvious pathological changes. In summary, our findings provide a new strategy for the prevention and control of avian colibacillosis.

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