数字农科院2.0

Genome-Wide Analysis Of Differentially Expressed Profiles Of Mrnas, Lncrnas And Circrnas In Chickens During Eimeria Necatrix Infection

文献类型： 外文期刊

作者： Fan, XC; Liu, TL; Wang, Y; Wu, XM; Wang, YX; Lai, P; Song, JK; Zhao, GH

作者机构：

关键词： Eimeria necatrix; mRNAs; lncRNAs; circRNAs; Chicken small intestine; Expression profile

期刊名称： PARASITES & VECTORS

ISSN： 1756-3305

年卷期： 2020 年 13 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： BackgroundEimeria necatrix, the most highly pathogenic coccidian in chicken small intestines, can cause high morbidity and mortality in susceptible birds and devastating economic losses in poultry production, but the underlying molecular mechanisms in interaction between chicken and E. necatrix are not entirely revealed. Accumulating evidence shows that the long-non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) are key regulators in various infectious diseases. However, the expression profiles and roles of these two non-coding RNAs (ncRNAs) during E. necatrix infection are still unclear.MethodsThe expression profiles of mRNAs, lncRNAs and circRNAs in mid-segments of chicken small intestines at 108 h post-infection (pi) with E. necatrix were analyzed by using the RNA-seq technique.ResultsAfter strict filtering of raw data, we putatively identified 49,183 mRNAs, 818 lncRNAs and 4153 circRNAs. The obtained lncRNAs were classified into four types, including 228 (27.87%) intergenic, 67 (8.19%) intronic, 166 (20.29%) anti-sense and 357 (43.64%) sense-overlapping lncRNAs; of these, 571 were found to be novel. Five types were also predicted for putative circRNAs, including 180 exonic, 54 intronic, 113 antisense, 109 intergenic and 3697 sense-overlapping circRNAs. Eimeria necatrix infection significantly altered the expression of 1543 mRNAs (707 upregulated and 836 downregulated), 95 lncRNAs (49 upregulated and 46 downregulated) and 13 circRNAs (9 upregulated and 4 downregulated). Target predictions revealed that 38 aberrantly expressed lncRNAs would cis-regulate 73 mRNAs, and 1453 mRNAs could be trans-regulated by 87 differentially regulated lncRNAs. Additionally, 109 potential sponging miRNAs were also identified for 9 circRNAs. GO and KEGG enrichment analysis of target mRNAs for lncRNAs, and sponging miRNA targets and source genes for circRNAs identified associations of both lncRNAs and circRNAs with host immune defense and pathogenesis during E. necatrix infection.ConclusionsTo the best of our knowledge, the present study provides the first genome-wide analysis of mRNAs, lncRNAs and circRNAs in chicken small intestines infected with E. necatrix. The obtained data will offer novel clues for exploring the interaction mechanisms between chickens and Eimeria spp.

分类号：

  • 相关文献

[1]Genome-wide expression profiling of mrnas, lncrnas and circrnas in skeletal muscle of two different pig breeds. Hou X.,Wang L.,Zhao F.,Zhang L.,Liu X.,Gao H.,Shi L.,Yan H.,Wang L.. 2021

[2]Matrine reduced intestinal stem cell damage in eimeria necatrix-infected chicks via blocking hyperactivation of Wnt signaling. Geng xiu Zan,Xiao fan Wang,Shao kang Yan,Ying chao Qin,Lang qun Yao,Chun qi Gao,Hui chao Yan,Jia yi Zhou,Xiu qi Wang. 2024

[3]Integrated Hypothalamic Transcriptome Profiling Reveals The Reproductive Roles Of Mrnas And Mirnas In Sheep. Zhang, ZB, Tang, JS, Di, R, Liu, QY, Wang, XY, Gan, SQ, Zhang, XS, Zhang, JL, Chu, MX, Hu, WP. 2020

[4]Circular RNA ciRS-7 affects the propagation of Cryptosporidium parvum in HCT-8 cells by sponging miR-1270 to activate the NF-κB signaling pathway. Yin Yan-Ling,Liu Ting-Li,Yao Qian,Wang Yu-Xin,Wu Xue-Mei,Wang Xue-Ting,Yang Xin,Song Jun-Ke,Zhao Guang-Hui. 2021

[5]Mirna And Circrna Expression Patterns In Mouse Brain During Toxoplasmosis Development. Zhou, CX, Ai, K, Huang, CQ, Guo, JJ, Cong, H, He, SY, Zhu, XQ. 2020

[6]Differential Expression Of Circular Rnas I.n Polytocous And Monotocous U terus During The Reproductive Cycle Of Sheep. La, YF, Tang, JS, Di, R, Wang, XY, Liu, QY, Zhang, LP, Zhang, XS, Zhang, JL, Hu, WP, Chu, MX. 2019

[7]Deciphering the ceRNA Network in Alfalfa: Insights into Cold Stress Tolerance Mechanisms. 朱琳,,赵宇杰,,郭茂伟,,白杰,,张良斌,,李志勇. 2026

[8]Long Non-Coding Rnas And Their P.otential Functions In Ligon-Lintless-1 M utant Cotton During Fiber Development. Salih, Haron,Salih, Haron,He, Shoupu,Gong, Wenfang,Du, Xiongming,Odongo, Magwanga Richard,Xia, Wang. 2019

[9]Genome-Wide Analysis Of Long Non-Coding R.nas In Potato And T heir Potential Role In Tuber Sprouting Process. Hou, XD, Du, YM, Liu, XM, Zhang, HB, Liu, YH, Yan, N, Zhang, ZF. 2018

[10]Insight of transcriptional regulators reveals the tolerance mechanism of carpet-grass (Axonopus compressus) against drought. Mohsin Nawaz,Liao Li,Farrukh Azeem,Samina Shabbir,Ali Zohaib,Umair Ashraf,Hubiao Yang,Zhiyong Wang. 2021

[11]Comparative Transcriptomics Reveal Key Sheep (.Ovis Aries) Hypothalamus Lncrnas T hat Affect Reproduction. Zhang, ZB, Tang, JS, Di, R, Liu, QY, Wang, XY, Gan, SQ, Zhang, XS, Zhang, JL, Hu, WP, Chu, MX. 2019

[12]Genome-Wide Identification of lncRNAs During Rice Seed Development. Juan Zhao , Abolore Adijat Ajadi , Yifeng Wang , Xiaohong Tong , Huimei Wang , Liqun Tang , Zhiyong Li , Yazhou Shu , Xixi Liu , Shufan Li , Shuang Wang , Wanning Liu , Jian Zhang *. 2020

[13]Molecular Evolution And Stress And P.hytohormone Responsiveness Of Sut G enes In Gossypium Hirsutum. Liu, Yangai,Ma, Xiongfeng,Pei, Xiaoyu,Wang, Zhenyu,Song, Chengxiang,Sun, Kuan,Yang, Daigang,Zhou, Xiaojian,Zhang, Fei,Ren, Zhongying,He, Kunlun,Li, Wei. 2018

[14]Gene Characterization And Expression Analysis R.eveal The Importance Of A uxin Signaling In Bud Dormancy Regulation In Tea Plant. Cao, Hongli,Chu, Jinfang,Fang, Shuang,Hao, Xinyuan,Hao, Xinyuan,Tang, Hu,Wang, Bo,Wang, Lu,Wang, Lu,Wang, Xinchao,Wang, Xinchao,Wang, Yuchun,Yang, Yajun,Yang, Yajun,Zeng, Jianming,Zeng, Jianming. 2019

[15]Transcriptome Sequencing And Whole Genome Expression Profiling Of Hexaploid Sweetpotato Under Salt Stress. Kou, Meng,Yan, Hui,Gao, Runfei,Wang, Xin,Ahmad, Muhammad Qadir,Aboelnasr, Hesham,Arisha, Mohamed Hamed,Li, Qiang,Zhang, Yungang,Arisha, Mohamed Hamed,Aboelnasr, Hesham,Tang, Wei,Liu, Yaju,Ahmad, Muhammad Qadir. 2020

[16]Molecular Cloning Of Wif1 And H.mga2 Reveals Ear-Preferential Expression W hile Uncovering A Missense Mutation Associated With Porcine Ear Size In Wif1. Liang, J, Zhang, Y, Wang, L, Liu, X, Yan, H, Wang, L, Zhang, L. 2019

[17]Genome-Wide Identification And Expression Analysis O.f The Biotin Carboxyl C arrier Subunits Of Heteromeric Acetyl-Coa Carboxylase In Gossypium. Cui, YP, Zhao, YP, Wang, YM, Liu, ZJ, Ijaz, B, Huang, Y, Hua, JP. 2017

[18]Genome-wide identification and analyses of the AHL gene family in cotton (Gossypium). Lanjie Zhao1† , Youjun Lü1,2,3† , Wei Chen1 , Jinbo Yao1 , Yan Li1 , Qiulin Li1 , Jingwen Pan1 , Shengtao Fang1, Jie Sun3* and Yongshan Zhang1*. 2020

[19]Molecular Cloning Of The B4Galnt2 G.ene And Its Single N ucleotide Polymorphisms Association With Litter Size In Small Tail Han Sheep. Guo, XF, Wang, XY, Liang, BM, Di, R, Liu, QY, Hu, WP, He, XY, Zhang, JL, Zhang, XS, Chu, MX. 2018

[20]Niemann-Pick Proteins Type C2 Are I.dentified As Olfactory Related G enes Of Pardosa Pseudoannulata By Transcriptome And Expression Profile Analysis. Zhang, Song,Xiao, Yong,Xiu, Chunli,Zhang, Yongjun,Xiu, Chunli,Xiao, Yong,Liu, Zewen,Bao, Haibo. 2019

作者其他论文 更多>>