A Novel Exopolysaccharide Produced By Lactobacillus Coryniformis Na-3 Exhibits Antioxidant And Biofilm-Inhibiting Properties In Vitro
文献类型: 外文期刊
作者: Xu, XQ; Peng, Q; Zhang, YW; Tian, DD; Zhang, PB; Huang, Y; Ma, L; Qiao, Y; Shi, B
作者机构:
关键词: Lactobacillus coryniformis NA-3; exopolysaccharide; antioxidant; anti-biofilm; inhibition; dispersion
期刊名称: FOOD & NUTRITION RESEARCH
ISSN: 1654-6628
年卷期: 2020 年 64 卷
页码:
收录情况: JCR(2021版)
摘要: Background: Exopolysaccharides (EPSs) secreted from lactic acid bacteria are carbohydrate polymers with reported biological activities. In this study, we extracted and characterized the composition as well as antioxidant and biofilm-inhibitory properties of EPS from Lactobacillus coryniformis NA-3 isolated from northeast Chinese sauerkraut (Suan Cai). Methods: Lactobacillus coryniformis NA-3 was identified with 16S rDNA amplification and Neighbor Joining (NJ) phylogenetic analysis. EPS derived from Lactobacillus coryniformis NA-3 (EPS-NA3) was analyzed, including compositions by high-performance liquid chromatography (HPI,C), functional groups by Fourier-transform infrared spectroscopy (FT-IR) and glycosidic bond configuration by Hydrogen-1 Nuclear Magnetic Resonance (H-1 NMR). Antioxidant activity of EPS was evaluated with hydroxyl and superoxide radical-scavenging. Anti-biofilm activities of EPS-NA3 were checked through inhibition and dispersion. Results: The monosaccharide composition of EPS included alpha-rhamnose, alpha-mannose, alpha-galactose, and alpha-glucose in a ratio of 2.6:1.0:5.0:3.3. The free radical-scavenging abilities of EPS-NA3 were 37.77% +/- 1.56% and 78.87% +/- 3.07% on hydroxyl and superoxide reactive oxygen species respectively. Moreover, EPS-NA3 attenuated the formation of Bacillus cereus and Salmonella typhimurium biofilms by inhibition ratios of approximately 80% and 40% respectively. Additionally, treatment with EPS-NA3 dispersed established biofilms of B. cereus and S. typhimurium by approximately 90% and 20% respectively. Conclusion: These results suggest that EPS-NA3 may be developed as antioxidant and anti-biofilm agents for industrial and clinical applications due to its capacity of scavenging free radicals, inhibition of bacterial biofilm formation, and dispersion of established biofilms.
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