数字农科院2.0

Enhanced Production Of Iturin A In Bacillus Amyloliquefaciens By Genetic Engineering And Medium Optimization

文献类型： 外文期刊

作者： Xu, YX; Cai, DB; Zhang, H; Gao, L; Yang, Y; Gao, JM; Li, YY; Yang, CL; Ji, ZX; Yu, J; Chen, SW

作者机构：

关键词： Bacillus amyloliquefaciens; Iturin A; Promoter replacement; AbrB; Medium optimization

期刊名称： PROCESS BIOCHEMISTRY

ISSN： 1359-5113

年卷期： 2020 年 90 卷

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Brown spot disease, caused by Alternaria alternate, is one of the most destructive leaf spot diseases that made a major impact on tobacco growing. Biocontrol has attracted increasing attentions as its features of environmental friendship, promoting plant growth, etc. Iturin A, with broad spectrum antifungal activity, is a kind of biosurfactant that mainly produced by Bacillus. In this study, the promoter of iturin A synthetase cluster of Bacillus amyloliquefaciens HZ-12 was respectively replaced by promoters P43, PbacA, PsrfA and Pylb, our results implied that transcriptional level of gene ituD and iturin A titer showed consistent change trends, and PbacA was proven as the most efficient promoter, iturin A titer of which reached 950.08 +/- 19.43 mg/L. Furthermore, regulator gene abrB was deleted to release the repression effect of AbrB on PbacA, ituD transcriptional level and iturin A titer were increased by 133.25 % and 20.88 %, respectively. Then, the maximum iturin A titer reached 2013.43 +/- 32.86 mg/L by optimizing fermentation medium, increased by 392.15 % compared to the original (408.97 +/- 21.35 mg/L). Finally, our results demonstrated that enhancing iturin A synthetic capability benefited the suppression of A. alternate. Collectively, this study provided a promising strain with an efficient fermentation medium for large-scale industrial production of iturin A.

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