数字农科院2.0

The Sweet Sorghum Sbwrky50 Is Negatively Involved In Salt Response By Regulating Ion Homeostasis

文献类型： 外文期刊

作者： Song, YS; Li, JL; Sui, Y; Han, GL; Zhang, Y; Guo, SJ; Sui, N

作者机构：

关键词： Sweet sorghum; SbWRKY50; Salt stress; SOS1; Ion homeostasis

期刊名称： PLANT MOLECULAR BIOLOGY

ISSN： 0167-4412

年卷期： 2020 年 102 卷 6 期

页码：

收录情况： JCR（2021版）

摘要： The WRKY transcription factor family is involved in responding to biotic and abiotic stresses. Its members contain a typical WRKY domain and can regulate plant physiological responses by binding to W-boxes in the promoter regions of downstream target genes. We identified the sweet sorghum SbWRKY50 (Sb09g005700) gene, which encodes a typical class II of the WRKY family protein that localizes to the nucleus and has transcriptional activation activity. The expression of SbWRKY50 in sweet sorghum was reduced by salt stress, and its ectopic expression reduced the salt tolerance of Arabidopsis thaliana plants. Compared with the wild type, the germination rate, root length, biomass and potassium ion content of SbWRKY50 over-expression plants decreased significantly under salt-stress conditions, while the hydrogen peroxide, superoxide anion and sodium ion contents increased. Real-time PCR results showed that the expression levels of AtSOS1, AtHKT1 and genes related to osmotic and oxidative stresses in over-expression strains decreased under salt-stress conditions. Luciferase complementation imaging and yeast one-hybrid assays confirmed that SbWRKY50 could directly bind to the upstream promoter of the SOS1 gene in A. thaliana. However, in sweet sorghum, SbWRKY50 could directly bind to the upstream promoters of SOS1 and HKT1. These results suggest that the new WRKY transcription factor SbWRKY50 participates in plant salt response by controlling ion homeostasis. However, the regulatory mechanisms are different in sweet sorghum and Arabidopsis, which may explain their different salt tolerance levels. The data provide information that can be applied to genetically modifying salt tolerance in different crop varieties. Key message (1) Sweet sorghum SbWRKY50 is negatively involved in salt response. (2) Over-expression of SbWRKY50 in A. thaliana affects plant growth, ROS and the ion contents. (3) SbWRKY50 could directly bind to the upstream promoter of the SOS1 gene in A. thaliana and the promoter of SOS1 and HKT1 in sweet sorghum.

分类号：

  • 相关文献

[1]Natural variations in SlSOS1 contribute to the loss of salt tolerance during tomato domestication. Zhen Wang,Yechun Hong,Yumei Li,Huazhong Shi,Juanjuan Yao,Xue Liu,Fuxing Wang,Sanwen Huang,Guangtao Zhu,Jian‐Kang Zhu. 2021

[2]Allelic variation of a soluble acid invertase gene (SAI-1) and development of a functional marker in sweet sorghum [Sorghum bicolor (L.) Moench]. Liu, Yang,Nie, Yuan-Dong,Han, Fen-Xia,Zhao, Xiang-Na,Dun, Bao-Qing,Lu, Ming,Li, Gui-Ying,Liu, Yang. 2014

[3]Production of ACE inhibitory peptides from sweet sorghum grain protein using alcalase: Hydrolysis kinetic, purification and molecular docking study. Wu, Qiongying,Du, Jinjuan,Jia, Junqiang,Kuang, Cong,Wu, Qiongying,Jia, Junqiang.

[4]Effects of Rumen Fluid as Bioaugmentation Additive on Fungal Diversity Dynamics during Sweet Sorghum Ensiling. Tian, Hui,Shi, Ruifeng,Wei, Huiyuan,Lu, Nana,Sun, Wenli,Ren, Haiwei,Zheng, Yi,Li, Jingping,Wang, Xiaoli. 2022

[5]Simplified methods of estimating fermentable sugar yield in sweet sorghum [Sorghum bicolor (L.) Moench] stems. Mei-Qi Yue,Zhi Wang,Bao-Qing Dun,Fen-Xia Han,Gui-Ying Li. 2021

[6]Zinc Stress Affects Ionome And M.etabolome In Tea Plants. Wang, Hui,Wang, Yu,Zhang, Yinfei,Song, Lubin,Ding, Zhaotang,Jia, Sisi,Ma, Dexin. 2017

[7]Mepiquat Chloride-Priming Induced Salt Tolerance D.uring Seed Germination Of C otton (Gossypium Hirsutum L.) Through Regulating Water Transport And K+/Na+ Homeostasis. Wang, Ning,Liu, Xiaohong,Wang, Xiangru,Xu, Qinghua,Shi, Jianbin,Yan, Gentu,Song, Meizhen,Zhou, Hong. 2019

[8]The Role of Membrane Transporters in Plant Growth and Development, and Abiotic Stress Tolerance. Rafaqat Ali Gill,Sunny Ahmar,Basharat Ali,Muhammad Hamzah Saleem,Muhammad Umar Khan,Weijun Zhou,Shengyi Liu. 2021

[9]Exogenous 2-(3,4-Dichlorophenoxy) Triethylamine Alleviates Salinity Stress In Maize By Enhancing Photosynthetic Capacity, Improving Water Status And Maintaining K+/Na(+)Homeostasis. Li, LJ, Gu, WR, Zhang, LG, Li, CF, Chen, XC, Qian, CR, Wang, ZH, Li, WH, Zuo, SY, Wei, S. 2020

[10]Advances in Sensing, Response and Regulation Mechanism of Salt Tolerance in Rice. Kimberly S. Ponce,Lijun Meng,Longbiao Guo,Yujia Leng,Guoyou Ye. 2021

[11]Exogenous Silicon Enhanced Salt Resistance By Maintaining K+/Na(+)Homeostasis And Antioxidant Performance In Alfalfa Leaves. Meng, YF, Yin, Q, Yan, ZJ, Wang, YQ, Niu, JM, Zhang, J, Fan, K. 2020

[12]Physiological, biochemical, and transcriptomic analyses revealed enhanced salt tolerance in rice via heterologous expression of Pyropia yezoensis APX gene. Xueli Lu,,Syeda Wajeeha Gillani,,Chen Meng,,Yiqiang Li,,Kexiang Wang,,Zongchang Xu. 2025

[13]Seed Germination Ecology Of Creeping M.annagrass (Glyceria Acutiflora) And R esponse To Post Herbicides. Xie, Yuan,Xie, Yuan,Tang, Wei,Lu, Yongliang,Chen, Jie. 2019

[14]Exogenous Melatonin Enhances Cold, Salt A.nd Drought Stress Tolerance B y Improving Antioxidant Defense In Tea Plant (Camellia Sinensis (L.) O. Kuntze). Li, Jiahao,Chen, Yi,Chen, Xuan,Sun, Kang,Li, Xinghui,Yang, Yiqing. 2019

[15]Modulation Of Ethylene And Ascorbic A.cid On Reactive Oxygen S pecies Scavenging In Plant Salt Response. Wang, Juan,Wang, Juan,Huang, Rongfeng,Huang, Rongfeng. 2019

[16]Rice Osdof15 Contributes To Ethylene-Inhibited P.rimary Root Elongation Under S alt Stress. Wang, Fangfang,Wang, Fangfang,Wang, Juan,Qin, Hua,Zhou, Jiahao,Wang, Juan,Zhang, Yugiong,Zhou, Yun,Huang, Rongfeng,Miao, Yuchen,Chen, Xinbing,Gao, Yadi,Qin, Hua,Huang, Rongfeng,Peng, Peng,Qi, Yidong. 2019

[17]Comparative Proteomic Analysis Of Two S.esame Genotypes With Contrasting S alinity Tolerance In Response To Salt Stress. Li, Donghua,Gong, Huihui,Liu, Aili,Zhang, Yujuan,Zhang, Yujuan,Zhou, Rong,Dossa, Komivi,Dossa, Komivi,Wei, Mengyuan,Wang, Linhai,You, Jun,Zhang, Yanxin,Zhang, Xiurong. 2019

[18]Genome-Wide Identification Of Lectin Receptor K.inases In Pear: Functional C haracterization Of The L-Type Lecrlk Gene Pblrk138. Ma, Na,Lin, Jing,Li, Hui,Liu, Chunxiao,Chang, Youhong,Wang, Jinyan,Zhang, Baolong. 2018

[19]The Response Of Transgenic Brassica S.pecies To Salt Stress: A Review. Zhang, Chunyu,Gong, Jianfang,Hou, Zhaoke,Khan, Shahbaz,Qian, Li,Salah, Akram,Shang, Zhengwei,Xu, Jingjing,Anwar, Sumera,Shah, Nadil. 2018

[20]Genome Wide Characterization, Evolution And Expression Analysis Of Fba Gene Family Under Salt Stress In Gossypium Species. Xu, Yanchao,Hou, Yuqing,Liu, Fang,Ditta, Allah,Wang, Kunbo,Yasir, Muhammad,Khan, Majid,Ditta, Allah,Shehzad, Muhammad,Cai, Xiaoyan,Wang, Xingxing,Zhou, Zhongli. 2019

作者其他论文 更多>>