数字农科院2.0

Contribution Of Calpain To Protein Degradation, Variation In Myowater Properties And The Water-Holding Capacity Of Pork During Postmortem Ageing

文献类型： 外文期刊

作者： Qian, SY; Li, X; Wang, H; Wei, XL; Mehmood, W; Zhang, CH; Blecker, C

作者机构：

关键词： Calpain; Water-Holding Capacity; Postmortem Storage; Protease Inhibitors; Myowater Distribution And Mobility

期刊名称： FOOD CHEMISTRY

ISSN： 0308-8146

年卷期： 2020 年 324 卷

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： To investigate calpain's effect on protein degradation, myowater properties, and the water-holding capacity (WHC), porcine longissimus muscles were incubated with control buffer, PD150,606 (calpain-specific inhibitor) and MG-262 (multiple-protease inhibitor) and assigned to an ageing period of 1, 4 or 7 d. Over 7 d of storage, no significant differences (P > 0.05) were observed in desmin or integrin expression between the MG-262 and PD150,606 groups, which indicated that calpain played a major role in protein proteolysis. Compared to those in the control group, muscle samples subjected to PD150,606 and MG-262 exhibited higher water mobility and a poorer WHC. Additionally, there were no significant differences in myowater properties or the WHC between the two groups at 1 d postmortem (P > 0.05). Calpain regulated the distribution and mobility of myowater, which contributed to a higher WHC in the early postmortem period (before 4 d), but other proteases tended to take over at a later stage.

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