数字农科院2.0

Heterogeneous Nuclear Ribonucleoprotein L Negatively Regulates Foot-And-Mouth Disease Virus Replication Through Inhibition Of Viral Rna Synthesis By Interacting With The Internal Ribosome Entry Site In The 5 ' Untranslated Region

文献类型： 外文期刊

作者： Sun, C; Liu, MM; Chang, JT; Yang, DC; Zhao, B; Wang, HW; Zhou, GH; Weng, CJ; Yu, L

作者机构：

关键词： Foot-And-Mouth Disease Virus; Internal Ribosome Entry Site; Heterogeneous Nuclear Ribonucleoprotein L; Viral Rna Synthesis; 3D(Pol); Virus-Host Interaction

期刊名称： JOURNAL OF VIROLOGY

ISSN： 0022-538X

年卷期： 2020 年 94 卷 10 期

页码：

收录情况： JCR（2021版）

摘要： Upon infection, the highly structured 5' untranslated region (5' UTR) of picornavirus is involved in viral protein translation and RNA synthesis. As a critical element in the 5' UTR, the internal ribosome entry site (IRES) binds to various cellular proteins to function in the processes of picornavirus replication. Foot-and-mouth disease virus (FMDV) is an important member in the family Picornaviridae, and its 5' UTR contains a functional IRES element. In this study, the cellular heterogeneous nu- clear ribonucleoprotein L (hnRNP was identified as an IRES-binding protein for FMDV by biotinylated RNA pulldown assays, mass spectrometry (MS) analysis, and determination of hnRNP L-IRES interaction regions. Further, we found that hnRNP L inhibited the growth of FMDV through binding to the viral IRES and that the inhibitory effect of hnRNP L on FMDV growth was not due to FMDV IRES-mediated translation, but to influence on viral RNA synthesis. Finally, hnRNP L was demonstrated to coimmunoprecipitate with RNA-dependent RNA polymerase (3D(pol)) in an FMDV RNA-dependent manner in the infected cells. Thus, our results suggest that hnRNP L, as a critical IRES-binding protein, negatively regulates FMDV replication by inhibiting viral RNA synthesis, possibly by remaining in the replication complex. IMPORTANCE Picornaviruses, as a large family of human and animal pathogens, cause a bewildering array of disease syndromes. Many host factors are implicated in the pathogenesis of these viruses, and some proteins interact with the viral IRES elements to affect function. Here, we report for the first time that cellular hnRNP L specifically interacts with the IRES of the picornavirus FMDV and negatively regulates FMDV replication through inhibiting viral RNA synthesis. Further, our results showed that hnRNP L coimmunoprecipitates with FMDV 3D(pol) in a viral RNA-dependent manner, suggesting that it may remain in the replication complex to function. The data presented here would facilitate further understanding of virus-host interactions and the pathogenesis of picornavirus infections.

分类号：

  • 相关文献

[1]Ribosomal Protein L13 Promotes Ires-Driven Translation Of Foot-And-Mouth Disease Virus In A Helicase Ddx3-Dependent Manner. Han, SC, Sun, SQ, Li, PH, Liu, Q, Zhang, ZH, Dong, H, Sun, MM, Wu, WX, Wang, XJ, Guo, HC. 2020

[2]Foot-And-Mouth Disease Virus Counteracts On I.nternal Ribosome Entry Site S uppression By G3Bp1 And Inhibits G3Bp1-Mediated Stress Granule Assembly Via Post-Translational Mechanisms. Ye, X, Pan, T, Wang, D, Fang, LR, Ma, J, Zhu, XY, Shi, YL, Zhang, KS, Zheng, HX, Chen, HC, Li, K, Xiao, SB. 2018

[3]The Ddx23 Negatively Regulates Translation And Replication Of Foot-And-Mouth Disease Virus And Is Degraded By 3C Proteinase. Abdullah, SW, Han, SC, Wu, JE, Zhang, Y, Bai, MY, Jin, Y, Zhi, XY, Guan, JY, Sun, SQ, Guo, HC. 2020

[4]Identification of the largest non-essential regions of the C-terminal portion in 3A protein of foot-and-mouth disease virus for replication in cell culture. . 2020

[5]VirusCircBase: a database of virus circular RNAs. Cai, Zena,Fan, Yunshi,Zhang, Zheng,Lu, Congyu,Zhu, Zhaozhong,Jiang, Taijiao,Shan, Tongling,Peng, Yousong. 2021

[6]Host Interaction Analysis Of Pa-N155 A.nd Pa-N182 In Chicken C ells Reveals An Essential Role Of Uba52 For Replication Of H5N1 Avian Influenza Virus. Wang, Q, Li, QH, Liu, T, Chang, GB, Sun, ZH, Gao, Z, Wang, F, Zhou, HJ, Liu, RR, Zheng, MQ, Cui, HX, Chen, GH, Li, H, Yuan, XY, Wen, J, Peng, DX, Zhao, GP. 2018

[7]Establishment of a Murine alpha nu beta 1 Transgenic CHO-K1 Cell Line and its Susceptibility to Foot-and-Mouth Disease Virus Type Asia 1/HN/2006 in China. Zhang, Y.,Zheng, H. X.,Zhang, Z. D.,Jin, Y.,Yang, F.,He, J. J.,Cao, W. J.,Sun, D. H.,Lv, L.. 2013

[8]Induction of systemic IFITM3 expression does not effectively control foot-and-mouth disease viral infection in transgenic pigs. Zhang, Huawei,Qian, Ping,Xu, Jinfang,Yang, Xi,Zhou, Rui,Chen, Huanchun,Li, Xiangmin,Zhang, Huawei,Qian, Ping,Xu, Jinfang,Yang, Xi,Zhou, Rui,Chen, Huanchun,Li, Xiangmin,Zheng, Haixue.

[9]Effect of the nucleotides surrounding the start codon on the translation of foot-and-mouth disease virus RNA. Ma, X. X.,Feng, Y. P.,Ma, Z. R.,Gu, Y. X.,Ma, X. X.,Zhou, J. H..

[10]Protection of a novel epitope-RNA VLP double-effective VLP vaccine for foot-and-mouth disease. Dong, Yan-mei,Chen, Liang,Dong, Yan-mei,Cai, Jian-chun,Chen, Hao-tai.

[11]Foot-and-mouth disease virus structural protein VP3 degrades Janus kinase 1 to inhibit IFN-gamma signal transduction pathways. Li, Dan,Yang, Fan,Liu, Hua-Nan,Zhu, Zi-Xiang,Cao, Wei-Jun,Liu, Xiang-Tao,Zheng, Hai-Xue,Wei, Jin,Li, Shu,Shu, Hong-Bing.

[12]Swine interferon-induced transmembrane protein, sIFITM3, inhibits foot-and-mouth disease virus infection in vitro and in vivo. Xu, Jinfang,Qian, Ping,Wu, Qunfeng,Liu, Shasha,Fan, Wenchun,Wang, Rong,Zhang, Huawei,Chen, Huanchun,Li, Xiangmin,Xu, Jinfang,Qian, Ping,Wu, Qunfeng,Liu, Shasha,Fan, Wenchun,Wang, Rong,Zhang, Huawei,Chen, Huanchun,Li, Xiangmin,Zhang, Keshan. 2014

[13]Techniques developed in china for foot-and-mouth disease diagnosis. Lu, Z.,Cao, Y.,Bao, H.,Qi, S.,Guo, J.,Shang, Y.,Jiang, T.,Zhang, Q.,Ma, J.,Liu, Z.,Liu, X.,Yin, H.,Xie, Q.. 2008

[14]Recombinant adenovirus expressing type Asia1 foot-and-mouth disease virus capsid proteins induces protective immunity against homologous virus challenge in mice. Zhou, Guohui,Wang, Haiwei,Wang, Fang,Yu, Li.

[15]EFFECT OF THE ROUTE OF FOOT-AND-MOUTH DISEASE VIRUS INFECTION OF PIGLETS ON THE COURSE OF DISEASE. Li, D.,Bai, X. -W.,Sun, P.,Fu, Y. -F.,Xie, B. -X.,Lu, Z. -J.,Chen, Y. -L.,Cao, W. -J.,Liu, Z. -X.. 2010

[16]Determination of Cattle Foot-and-Mouth Disease Virus by Micro-ELISA Method. Dong, Yiyang,Xu, Yan,Xu, Yan,Mawatari, Kazuma,Kitamori, Takehiko,Liu, Zaixin,Fu, Yuanfang,Ohashi, Toshinori,Mawatari, Kazuma,Kitamori, Takehiko.

[17]Purification of foot-and-mouth disease virus by heparin as ligand for certain strains. Du, Ping,Sun, Shiqi,Dong, Jinjie,Zhi, Xiaoying,Chang, Yanyan,Teng, Zhidong,Guo, Huichen,Liu, Zaixin.

[18]The effects of the synonymous codon usage and tRNA abundance on protein folding of the 3C protease of foot-and-mouth disease virus. Zhou, Jian-hua,You, Ya-nan,Chen, Hao-tai,Zhang, Jie,Ma, Li-na,Ding, Yao-zhong,Liu, Yong-sheng,Pejsak, Zygmunt.

[19]A novel bi-functional DNA vaccine expressing VP1 protein and producing antisense RNA targeted to 5 ' UTR of foot-and-mouth disease virus can induce both rapid inhibitory effect and specific immune response in mice. Yang, Bin,Li, Yanmin,Zhang, Zhidong,Yang, Bin,Lan, Xi,Li, Xueri,Yin, Xiangping,Li, Baoyu,Han, Xiaorong,Li, Yanmin,Zhang, Zhidong,Liu, Jixing. 2008

[20]Esterase D enhances type I interferon signal transduction to suppress foot-and-mouth disease virus replication. Li, Weiwei,Zhu, Zixiang,Cao, Weijun,Yang, Fan,Zhang, Xiangle,Li, Dan,Zhang, Keshan,Li, Pengfei,Mao, Ruoqing,Liu, Xiangtao,Zheng, Haixue.

作者其他论文 更多>>