数字农科院2.0

Comparison Of Immune Responses In Guinea Pigs By Intranasal Delivery With Different Nanoparticles-Loaded Fmdv Dna Vaccine

文献类型: 外文期刊

作者: Zheng, HB; Pan, L; Lv, JL; Zhang, ZW; Wang, YY; Hu, WF; Liu, XS; Zhou, P; Wang, YL; Zhang, YG

作者机构:

关键词: Mucosal Immune; Chitosan; Poly (Lactic-Co-Glycolic) Acid; Silica

期刊名称: MICROBIAL PATHOGENESIS

ISSN: 0882-4010

年卷期: 2020 年 142 卷

页码:

收录情况: JCR(2021版)

摘要: To compare different nanoparticle-based nasal vaccines against foot-and-mouth disease (FMD), chitosan (CS)coated poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) (CS/PLGA-NPs) and amino-functionalized mesoporous silica nanoparticles (Am/MSNs) loaded with FMDV recombinant plasmid (pP12A3C/IFN-CS/PLGA-NPs and pP12A3C/IFN-Am/MS-NPs) were used to induce mucosal and systemic immune responses in guinea pigs via intranasal delivery. Simultaneously, CpG oligodeoxy nucleotides (ODNs) as a vaccine adjuvant were encapsulated in chitosan-coated poly (lactic-co-glycolic acid) nanoparticles (CpG-CS/PLGA-NPs). The pP12A3C/IFN-CS/PLGA-NPs and CpG-CS/PLGA-NPs generated displayed good morphology, high stability, mean diameters of 500 and 400 nm and encapsulation efficiencies of 83.8% and 88.4%, respectively. Data from the in vitro release assay showed that plasmid and CpG were sustainably released from nanoparticles (up to 66.73% and 64%, respectively, of the total amount loaded). Guinea pigs immunized with pP12A3C/IFN-CS/PLGANPs + CpG-CS/PLGA-NPs showed markedly higher mucosal, cellular and humoral immune responses than those administered pP12A3C/IFN-CS/PLGA-NPs or naked plasmid vaccine alone. FMDV-specific secretory immunoglobulin A (sIgA) antibodies in nasal washes were initially detected at 3 days post-vaccination with CS/ PLGA-NPs loaded with plasmid. Guinea pigs immunized with pP12A3C/IFN-CS/PLGA-NPs also displayed higher cellular and humoral immune responses than pP12A3C-CS/PLGA-NPs and naked plasmid vaccine alone. FMDV-specific immunoglobulin G (IgG) antibodies in serum were initially detected at 5 days post-vaccination (intramuscularly) with the naked plasmid. Finally, challenge experiments 42 days post-vaccine revealed 100% protection in guinea pigs immunized with pP12A3C/IFN-CS/PLGA-NPs + CpG-CS/PLGA-NPs and pP12A3C/IFN-CS/PLGA-NPs. However, plasmid DNA was burst released from pP12A3C/IFN-Am/MS-NPs. Our attempts to use pP12A3C/IFN-Am/MS-NPs to immunize guinea pigs failed to induce immune responses. In conclusion, CpG and IFN-alpha adjuvant based FMD vaccines elicit protection in guinea pigs. Moreover, CS-coated PLGA NPs present an efficient and safe mucosal immune delivery system for FMDV DNA vaccine. Data from the current study provide a foundation for understanding and further evaluating protective immune responses in pigs.

分类号:

  • 相关文献

[1]Analysis Of Xanthine Oxidase Inhibitors F.rom Clerodendranthus Spicatus With X anthine Oxidase Immobilized Silica Coated Fe3O4 Nanoparticles. Liu, LL, Yuan, MM, Huang, SQ, Li, JJ, Li, DF, Zhao, LN. 2018

[2]Vapor–Liquid Transition-Based Broadband Light Modulation for Self-Adaptive Thermal Management. Zhang C., Yang J., Li Y., Song J., Guo J., Fang Y., Yang X., Yang Q., Wang D., Deng X.. 2022

[3]Synchronous pressing and refining after solid-phase preadsorption technology as a new method for rapeseed oil preparation. Wang W., Yang B., Huang F., Zheng C., Li W., Liu T., Liu C.. 2022

[4]Biodegradable and self-fluorescent ditelluride-bridged mesoporous organosilica/polyethylene glycol-curcumin nanocomposite for dual-responsive drug delivery and enhanced therapy efficiency. Xia X., Shi J., Deng Q., Xu N., Huang F., Xiang X.. 2022

[5]Coagulation Of Low Temperature And L.ow Turbidity Water: Adjusting B asicity Of Polyaluminum Chloride (Pac) And Using Chitosan As Coagulant Aid. Chang, Xing,Qian, Jin,Zhang, Zhen,Li, Yongtao,Ma, Guilin,Jing, Ran,Li, Yongtao,Zhang, Kun,He, Shuran,Zhang, Mingkuan. 2018

[6]Nitrogen-Doped Porous Carbon Derived From C.hitosan For The Enhanced D ehydrochlorination Of Lindane Under Mild Conditions. Yang, Jirui,Shen, Feng,Qiu, Mo,Qi, Xinhua. 2018

[7]Modification Of 13X Molecular Sieve B.y Chitosan For Adsorptive R emoval Of Cadmium From Simulated Wastewater. Li, Xiuxiu,Sun, Ken,Shi, Yan,Qi, Xuebin,Huo, Lixin,Li, Zhaohui. 2017

[8]Effect Of Electrostatically Charged And N.eutral Polysaccharides On The R heological Characteristics Of Peanut Protein Isolate After High-Pressure Homogenization. Jiao, B, Shi, AM, Liu, HZ, Sheng, XJ, Liu, L, Hu, H, Adhikari, B, Wang, Q. 2018

[9]Recyclable Ferromagnetic Chitosan Nanozyme For D.ecomposing Phenol. Jiang, JF, He, CY, Wang, S, Jiang, H, Li, JD, Li, LS. 2018

[10]Structural And Biochemical Insights Into T.he Catalytic Mechanisms Of T wo Insect Chitin Deacetylases Of The Carbohydrate Esterase 4 Family. Liu, L, Zhou, Y, Qu, MB, Qiu, Y, Guo, XM, Zhang, YB, Liu, T, Yang, J, Yang, Q. 2019

[11]Pretreatment Of Poultry Waste Anaerobic D.igested Effluents By Chitosan F locculation For Chlorella Pyrenoidosa Growth And Pollutants Removal. Wu, Y, Wang, MZ, Zhang, H, Cao, W, Liu, ZD, Lu, HF. 2017

[12]Studies On The Chitin/Chitosan Binding P.roperties Of Six Cuticular P roteins Analogous To Peritrophin 3 From Bombyx Mori. Ren, Y.,Yang, Q.,Qu, M.,Yang, Q.,Liu, Y.. 2017

[13]Preparation and characterization of chitosan/Nano-ZnO composite film with antimicrobial activity. Xiyue Zhang,Zheng Zhang,Wenyi Wu,Jun Yang,Qing Yang. 2021

[14]Antifungal activity of chitosan against Phytophthora infestans, the pathogen of potato late blight. Xiaoqing Huang,Ziyue You,Yang Luo,Chengji Yang,Jie Ren,Yanlin Liu,Guangjing Wei,Pan Dong,Maozhi Ren. 2021

[15]Complete dechlorination of lindane over N-doped porous carbon supported Pd catalyst at room temperature and atmospheric pressure. Qi, Xinhua,Smith, Richard Lee, Jr.,Qiu, Mo,Yang, Jirui,Shen, Feng. 2020

[16]Electrochemical Antioxidant Screening And Evaluation Based On Guanine And Chitosan Immobilized Mos2 Nanosheet Modified Glassy Carbon Electrode (Guanine/Cs/Mos2/Gce). Tang, P, Tang, XS, Mei, SY, Xie, YX, Liu, LL, Ren, LC. 2020

[17]Artificial Esterase Based On Self-Assembly G.el Microspheres Constructed From C hitosan And Amino Acids. Cao, J, Wang, M, Chen, WH, She, YX, Wang, J, Wang, FZ, Lao, SB. 2019

[18]Preparation and characterization of chitosan/Nano-ZnO composite film with antimicrobial activity. Xiyue Zhang,Zheng Zhang,Wenyi Wu,Jun Yang,Qing Yang. 2021

[19]Modulation Effects Of Toxoplasma Gondii Histone H2A1 On Murine Macrophages And Encapsulation With Polymer As A Vaccine Candidate. Yu, ZQ, Zhou, TY, Luo, YX, Dong, L, Li, CJ, Liu, JL, Luo, JX, Yan, RF, Xu, LX, Song, XK, Li, XR. 2020

作者其他论文 更多>>