数字农科院2.0

Quantitative Phosphoproteomics Analysis Of Actomyosin Dissociation Affected By Specific Site Phosphorylation Of Myofibrillar Protein

文献类型： 外文期刊

作者： Cao, LC; Hou, CL; Hussain, Z; Zhang, DQ; Wang, ZY

作者机构：

关键词： Myofibrillar Proteins; Myosin; Actomyosin Dissociation; Phosphorylation; Phosphoproteomics

期刊名称： LWT-FOOD SCIENCE AND TECHNOLOGY

ISSN： 0023-6438

年卷期： 2020 年 126 卷

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Phosphorylation of myofibrillar proteins can regulate muscle contraction and thus affect actomyosin dissociation. To explore the mechanism by which myofibrillar protein phosphorylation and phosphorylation site affects actomyosin dissociation, mass spectrometry-based quantitative phosphoproteomics was used to analyze ovine longissimus thoracic muscles with different actomyosin dissociation. A total of 2073 phosphorylated peptides from 826 phosphoproteins were identified among the myofibrillar proteins of muscles with different actomyosin dissociation, and 176 phosphorylated peptides from 114 phosphoproteins were significantly different (P < 0.05) between the high and low group of actomyosin dissociation. Phosphorylation of myosin heavy chain 1 at Ser1896, myosin heavy chain 7 at Thr1019 and Tyr1307, and thin at Ser265, Ser6085, Ser21930, and Ser26576 can inhibit actomyosin dissociation, whereas phosphorylation of tropomyosin 2 at Ser86, Thr252, and Ser279 contribute to actomyosin dissociation. The phosphorylation sites associated with actomyosin dissociation were mostly serine residues, and the phosphorylation status of myosin regulatory light chain at Ser15 was negatively correlated with actomyosin dissociation. In conclusion, the phosphorylation of some myofibrillar proteins and myosin at specific serine residues may be crucial for actomyosin dissociation.

分类号：

  • 相关文献

[1]Effects of acetylation on dissociation and phosphorylation of actomyosin in postmortem ovine muscle during incubation at 4 °C in vitro. Zhang Y.,Li X.,Zhang D.,Bai Y.,Wang X.. 2021

[2]Mitogen-Activated Protein Kinases Associated Sites of Tobacco Repression of Shoot Growth Regulates Its Localization in Plant Cells. Wang L., Gui Y., Yang B., Dong W., Xu P., Si F., Yang W., Luo Y., Guo J., Niu D., Jiang C.. 2022

[3]DIA-based quantitative proteomic analysis on the meat quality of porcine Longissimus thoracis et lumborum cooked by different procedures. Song Y., Huang F., Li X., Zhang H., Liu J., Han D., Rui M., Wang J., Zhang C.. 2022

[4]Foot-And-Mouth Disease Virus Counteracts On I.nternal Ribosome Entry Site S uppression By G3Bp1 And Inhibits G3Bp1-Mediated Stress Granule Assembly Via Post-Translational Mechanisms. Ye, X, Pan, T, Wang, D, Fang, LR, Ma, J, Zhu, XY, Shi, YL, Zhang, KS, Zheng, HX, Chen, HC, Li, K, Xiao, SB. 2018

[5]Quantitative Phosphoproteomic And Metabolomic Analyses R.eveal Gmmyb173 Optimizes Flavonoid M etabolism In Soybean Under Salt Stress. Pi, EX, Zhu, CM, Fan, W, Huang, YY, Qu, LQ, Li, YY, Zhao, QY, Ding, F, Qiu, LJ, Wang, HZ, Poovaiah, BW, Du, LQ. 2018

[6]Quantitative Phosphoproteomic Analysis Among Muscles O.f Different Color Stability U sing Tandem Mass Tag Labeling. Li, Z, Li, M, Li, X, Xin, JZ, Wang, Y, Shen, QWW, Zhang, DQ. 2018

[7]Global phosphoproteome analysis reveals significant differences between sporulated oocysts of virulent and avirulent strains of Toxoplasma gondii. Wang Z.-X.,Hu R.-S.,Zhu X.-Q.,Sun X.-L.,Elsheikha H.M.. 2021

[8]Phosphoproteomic basis of neuroplasticity in the antennal lobes influences the olfactory differences between A. mellifera and A. cerana honeybees. Meng L., Altaye S.Z., Feng M., Hu H., Han B., Li J.. 2022

[9]Itraq-Based Global Phosphoproteomics Reveals Novel M.olecular Differences Between Toxoplasma G ondii Strains Of Different Genotypes. Wang, ZX, Zhou, CX, Calderon-Mantilla, G, Petsalaki, E, He, JJ, Song, HY, Elsheikha, HM, Zhu, XQ. 2019

[10]Phosphorylation of myofibrillar proteins in post-mortem ovine muscle with different tenderness. Chen, Lijuan,Li, Xin,Ni, Na,Liu, Yue,Chen, Li,Wang, Zhenyu,Shen, Qingwu W.,Zhang, Dequan,Zhang, Dequan.

[11]Effects Of Konjac Glucomannan And A.cetylated Distarch Phosphate On T he Gel Properties Of Pork Meat Myofibrillar Proteins. Tong, QY, Chen, L, Wang, WJ, Zhang, ZP, Yu, XP, Ren, F. 2018

[12]Structural changes induced by ultrasound improve the ability of the myofibrillar protein to bind flavor compounds from spices. Xiangxiang Sun,Yumei Yu,Ahmed S.M. Saleh,Xinyu Yang,Jiale Ma,Ziwu Gao,Wenhao Li,Zhenyu Wang,Dequan Zhang. 2023

[13]Exploring the interaction between myofibrillar proteins and pyrazine compounds: Based on molecular docking, molecular dynamics simulation, and multi-spectroscopy techniques. Yumei Yu,Ahmed S.M. Saleh,Xiangxiang Sun,Zhenyu Wang,Yang Lu,Dequan Zhang,Chunjiang Zhang. 2023

[14]Sarcoplasmic and myofibrillar phosphoproteins profile of beef M. longissimus thoracis with different pH(u) at different days postmortem. Ijaz, Muawuz,Li, Xin,Zhang, Dequan,Bai, Yuqiang,Hou, Chengli,Hussain, Zubair,Zheng, Xiaochun,Huang, Caiyan. 2021

[15]Effect of static magnetic field-assisted thawing on the quality, water status, and myofibrillar protein characteristics of frozen beef steaks. Wang, Wenxin,Lin, Hengxun,Guan, Wenqiang,Song, Yu,He, Xingxing,Zhang, Dequan. 2024

[16]Investigating the interactions between selected heterocyclic flavor compounds and beef myofibrillar proteins using SPME-GC–MS, spectroscopic, and molecular docking approaches. Meng Wei,Xiaochang Liu,Peng Xie,Aiyun Han,Yuanhua Lei,Xiaodong Yang,Yinchu Liu,Songshan Zhang,Baozhong Sun. 2024

[17]Effect of interaction between resveratrol and myofibrillar protein on the production of bound heterocyclic amines. Xiaoyue Yang,Dequan Zhang,Christophe Blecker,Chunjiang Zhang,Xiangxiang Sun,Zhenyu Wang. 2024

[18]Apoptosis and its role in postmortem meat tenderness: A comprehensive review. Zhao, Yingxin,Xiang, Can,Roy, Bimol C.,Bruce, Heather L.,Blecker, Christophe,Zhang, Yanyan,Liu, Chongxin,Zhang, Dequan,Chen, Li,Huang, Caiyan. 2024

[19]Effects of acidic and alkaline pH-shifting on structural and digestion characteristics of myofibrillar proteins of beef muscles. Min You,Yanyan Chen,Chunhui Zhang,Feng Huang. 2025

[20]Interactions between lycopene and myofibrillar proteins under different pH conditions: Implications for structural properties and in vitro digestibility. Jia Huang,Min You,Yanyan Chen,Chunhui Zhang,Feng Huang. 2026

作者其他论文 更多>>