数字农科院2.0

Decreasing of S100A4 in bovine endometritis in vivo and in vitro

文献类型： 外文期刊

作者： Li, Yajuan;Li, Yajuan;Zhang, Shidong;Yan, Zuoting;Szenci, Otto;Shao, Dan;Wang, Dongsheng;Wu, Xiaohu;He, Baoxiang;Song, Pengjie;Cheng, Zhangrui

作者机构：

关键词： S100A4; Endometritis; Dairy Cow

期刊名称： Theriogenology

ISSN： 0093-691X

年卷期： 2020 年 153 卷

页码：

收录情况： JCR（2021版）

摘要： Endometritis is a prevalent reproductive disease in dairy cows, and is a superficial inflammation of the endometrium. 5100 calcium-binding protein A4 (S100A4) is suggested to be implicated in the progression of inflammation. However, to our knowledge, no study has reported the changes of S100A4 during bovine endometritis. The objective of this study was to investigate S100A4 gene expression and protein levels in the uterus with endometritis in dairy cows. Vaginal mucus samples were collected for diagnosis of the severity degree of endometritis and the detection of S100A4 protein content. Blood samples and endometrial biopsies were collected and divided into the control (CH), mild endometrtis (M), and severe endometritis (S) groups according to the characteristics of the vaginal mucus type. The isolated bovine endometrial epithelial cells (BEECs) were challenged with E. soli (2 x 106 CFU/mL, 2 x 107 CFU/mL) or lipopolysaccharide (LPS, 3 and 10 ttg/mL) as an inflammatory model. RT-qPCR was used to detect the gene expression levels of S100A4 and cytokines, including interleukin-113 (IL-1(3), interleukin-6 (IL-6), interleukin-10 (IL-10), and tumour necrosis factor-alpha (THE-a), in tissues or cells. Enzyme-linked immunosorbent assay (ELISA) was used for S100A4 protein level detection in tissues, cells, cell supernatant, vaginal mucus, and serum samples. The results showed that S100A4 gene and protein levels decreased in bovine endometrium with endometritis and in E. soli- or LPS-stimulated BEECs. We failed to detect S100A4 in the cell supernatant, vaginal mucus, and serum samples. This study suggested that S100A4 is a pathogenesis-related protein of endometritis, and decreased expression of S100A4 may pave the way for the development of endometritis in dairy cows. 0 (C) 2020 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]梅花鹿鹿茸S100A4融合蛋白在大肠杆菌中的表达纯化. 刘东,邢秀梅,赵海平,褚文辉,鲁晓萍,王大涛,李春义. 2011

[2]S100A4 regulates ferroptosis to suppress cell proliferation via the SLC7A11-GSH axis but facilitates Brucella infection in RAW264.7 macrophages. Guangdong Zhang,Yi Yin,Hai Hu,Mingxing Tian,Chan Ding,Shengqing Yu. 2025

[3]Endometrial Expression Of The Acute P.hase Molecule Saa Is M ore Significant Than Hp In Reflecting The Severity Of Endometritis. Dong, Shuwei,Yan, Zuoting,Oguejiofor, Chike F.,Zhang, Shidong,Zhang, Shidong,Wang, Dongsheng,Yang, Feng. 2018

[4]Microrna bta-mir-24-3p suppressed galectin-9 expression through tlr4/nf-kbsignaling pathway in lps-stimulated bovine endometrial epithelial cells. Oladejo A.O.,Li Y.,Shen W.,Imam B.H.,Wu X.,Yang J.,Ma X.,Lv Y.,Jiang W.,Ding X.,Wang S.,Yan Z.. 2021

[5]Anti-Inflammatory Effect Of Astragalin And Chlorogenic Acid On Escherichia Coli-Induced Inflammation Of Sheep Endometrial Epithelium Cells. Hu, XQ, Wang, M, Pan, YY, Xie, YY, Han, JH, Zhang, XY, Niayale, R, He, HH, Li, Q, Zhao, T, Cui, Y, Yu, SJ. 2020

[6]Treatment Of The Retained Placenta I.n Dairy Cows: Comparison O f A Systematic Antibiosis With An Oral Administered Herbal Powder Based On Traditional Chinese Veterinary Medicine. Wang, Hui,Wang, Hui,Tuo, Xin,Tuo, Xin,Wang, Shengyi,Wang, Shengyi,Wang, Lei,Wang, Lei,Huang, Xueli,Huang, Xueli,Cui, Dongan,Cui, Dongan,Liu, Yongming,Liu, Yongming,Li, Jianxi,Li, Jianxi. 2017

[7]The Particulate Passage Rate, Nutrient C.omposition And Fermentation Characteristics A cross Gastrointestinal Tracts In Lactating Dairy Cows Fed Three Different Forage Source Diets. Wang, B, Gu, FF, Huang, XB, Liu, JX. 2018

[8]Effects Of Feeding A Moringa O.leifera Rachis And Twig P reparation To Dairy Cows On Their Milk Production And Fatty Acid Composition, And Plasma Antioxidants. Zhang, TT, Si, BW, Deng, KD, Tu, Y, Zhou, CL, Diao, QY. 2018

[9]Hydroxy-Selenomethionine: A Novel Organic Selenium S.ource That Improves Antioxidant S tatus And Selenium Concentrations In Milk And Plasma Of Mid-Lactation Dairy Cows. Sun, P, Wang, J, Liu, W, Bu, DP, Liu, SJ, Zhang, KZ. 2017

[10]Administration Of An Herbal Powder B.ased On Traditional Chinese V eterinary Medicine Enhanced The Fertility Of Holstein Dairy Cows Affected With Retained Placenta. Huang, XL, Wang, SY, Wang, L, Wang, H, Li, X, Cui, DG. 2018

[11]Role Of Age-Related Shifts In R.umen Bacteria And Methanogens I n Methane Production In Cattle. Liu, C, Meng, QH, Chen, YX, Xu, MS, Shen, M, Gao, R, Gan, SQ. 2017

[12]An overview of aflatoxin B1 biotransformation and aflatoxin M1 secretion in lactating dairy cows. Li Min,Johanna Fink-Gremmels,Dagang Li,Xiong Tong,Jing Tang,Xuemei Nan,Zhongtang Yu,Weidong Chen,Gang Wang. 2021

[13]Short Communication: Effects Of Different S.elenium Supplements On Rumen F ermentation And Apparent Nutrient And Selenium Digestibility Of Mid-Lactation Dairy Cows. Wei, JY, Wang, J, Liu, W, Zhang, KZ, Sun, P. 2019

[14]Biological System Responses Of Dairy C.ows To Aflatoxin B1 E xposure Revealed With Metabolomic Changes In Multiple Biofluids. Wang, Q, Zhang, YD, Zheng, N, Guo, LY, Song, XM, Zhao, SG, Wang, JQ. 2019

[15]The Biochemical And Metabolic Profiles Of Dairy Cows With Mycotoxins-Contaminated Diets. Wang, Q, Zhang, YD, Zheng, N, Zhao, SG, Li, SL, Wang, JQ. 2020

[16]Research On The Applications Of Calcium Propionate In Dairy Cows: A Review. Zhang, F, Nan, XM, Wang, H, Guo, YM, Xiong, BH. 2020

[17]Estimation Of Between-Cow Variability In Nutrient Digestion Of Lactating Dairy Cows Fed Corn-Based Diets. Tharangani, H, Lu, CW, Zhao, LS, Ma, L, Guo, XS, Weiss, WP, Bu, DP. 2020

作者其他论文 更多>>