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Overexpression Ofmedicago Sativa Lea4-4 Can Improve The Salt, Drought, And Oxidation Resistance Of Transgenic Arabidopsis

文献类型： 外文期刊

作者： Jia, HL; Wang, XM; Shi, YH; Wu, XM; Wang, YQ; Liu, JN; Fang, ZH; Li, CY; Dong, KH

作者机构：

期刊名称： PLOS ONE

ISSN： 1932-6203

年卷期： 2020 年 15 卷 6 期

页码：

收录情况： JCR（2021版）

摘要： Late embryogenesis abundant (LEA) proteins are widely involved in many adverse conditions among plants. In this study, we isolated aLEA4 gene from alfalfa (Medicago sativaL.) termedMsLEA4-4 via a homology cloning strategy.MsLEA4-4 encodes 166 amino acids, and the structural analysis showed that the gene contained five repeating TAQAAKEKTQQ amino acid motifs. There were a large number of alpha-helix inMsLEA4-4, and belongs to hydrophilic amino acid. Subcellular localization analysis showed thatMsLEA4-4 was localized in the nucleus. TheMsLEA4-4 promoter consisted of G-box and A-box elements, abscisic acid-responsive elements (ABREs), photo regulation and photoperiodic-controllingcis-acting elements, and endosperm expression motifs. TheMsLEA4-4 overexpressing inArabidopsisconferred late-germination phenotypes. Resistance of the overexpressed plants to abiotic stress significantly outperformed the wild-type (WT) plants. Under salt stress and abscisic acid treatment, with more lateral roots and higher chlorophyll content, the overexpressed plants has a higher survival rate measured against WT. Compared to those in the WT plants, the levels of soluble sugar and the activity of various antioxidant enzymes were elevated in the overexpressed plants, whereas the levels of proline and malondialdehyde were significantly reduced. The expression levels of several genes such asABF3,ABI5,NCED5, andNCED9 increased markedly in the overexpressed plants compared to the WT under osmotic stress.

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