数字农科院2.0

Cry80Aa1, A Novel Bacillus Thuringiensis Toxin With Mosquitocidal Activity To Culex Pipiens Pallens

文献类型： 外文期刊

作者： Zhou, Y; Wu, ZQ; Zhang, J; 宛煜嵩; 金芜军; Li, YZ; Fang, XJ

作者机构：

关键词： Bacillus Thuringiensis; Cry80Aa1; Toxin_10; Culex Pipiens Pallens

期刊名称： JOURNAL OF INVERTEBRATE PATHOLOGY

ISSN： 0022-2011

年卷期： 2020 年 173 卷

页码：

收录情况： JCR（2021版）

摘要： Bacillus thuringiensis (Bt) can synthesize insecticides to efficiently control insects. In this study, Bt strain S3580-1 with mosquitocidal activity was subjected to whole genome sequencing using an Illumina HiSeq 2000 system. A novel toxin, Cry80Aa1, was identified based on the resulting data. A conserved domain analysis revealed that Cry80Aa1 includes the Ricin_B _lectin domain (located at 10-150) and the Toxin_10 domain (located at 155-353). Phylogenetic tree analysis showed that Cry80Aa1 was in a distinct Glade significantly distinguished from the known Cry proteins containing the Toxin_10 domain. Bioassays demonstrated that the Cry80Aa1 protein exhibited toxicity to third instar larvae of Culex pipiens pallens (LC50: 71.9 mu g/mL; 95% FL: 59.5-122.7 mu g/mL).

分类号：

  • 相关文献

[1]Resistance alleviation in the larval cotton bollworm to fenvalerate after pretreatment with bacillus thuringiensis. 谭维嘉,梁革梅,郭予元. 1999

[2]利用ubi1内含子增强Bt cry1Ah基因在转基因玉米中的表达. 郎志宏,张杰,何康来,宋福平,黄大昉. 2008

[3]棉铃虫中肠氨肽酶APN4与Cry1Ac、Cry2Aa结合能力的比较. 葛朝虹,肖玉涛,梁革梅. 2015

[4]产Zwittermicin A的苏云金芽孢杆菌菌株的筛选及抗性基因zmaR的克隆与表达. 宋福平,李卓夫,张杰,姜声华,刘大群,黄大昉. 2005

[5]The Cadherin Protein Is Not Involved In Susceptibility Tobacillus Thuringiensiscry1Ab Or Cry1Fa Toxins Inspodoptera Frugiperda. Gomez, Isabel,Yang, Yanchao,Yang, Yongbo,Liu, Kaiyu,Liu, Leilei,Zhang, Jianfeng,Jin, Minghui,Bravo, Alejandra,Xiao, Yutao,Soberon, Mario. 2020

[6]Bt Proteins Have No Detrimental E.ffects On Larvae Of T he Green Lacewing, Chrysopa Pallens (Rambur) (Neuroptera: Chrysopidae). Muhammad, M. S.,Zhang, S.,Ali, I.,Iqbal, M.,Ali, I.,Ali, I.,Cui, J. J.,Ali, I.. 2018

[7]PROTEOMIC ANALYSIS OF NOVEL Cry1Ac BINDING PROTEINS IN Helicoverpa armigera (HUBNER). Chen, Li-Zhen,Liang, Ge-Mei,Zhang, Jie,Wu, Kong-Ming,Guo, Yu-Yuan,Rector, Brian G.. 2010

[8]Feeding behaviour of Helicoverpa armigera larvae on insect-resistant transgenic cotton and non-transgenic cotton. Zhang, JH,Wang, CZ,Qin, JD,Guo, SD. 2004

[9]Processing of delta-endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1 in Heliothis armigera midgut juice and the effects of protease inhibitors. Shao, ZZ,Cui, YL,Liu, XL,Yi, HQ,Ji, JH,Yu, ZN. 1998

[10]Bt Toxin Cry1Ie Causes No Negative Effects on Survival, Pollen Consumption, or Olfactory Learning in Worker Honey Bees (Hymenoptera: Apidae). Diao, Qing-Yun,Jia, Hui-Ru,Geng, Li-Li.

[11]Reproductive Cost Associated With Juvenile Hormone in Bt-Resistant Strains of Helicoverpa armigera (Lepidoptera: Noctuidae). Wang, B. J.,Chen, L.,Khaing, M. M.,Lu, Y. H.,Liang, G. M.,Guo, Y. Y.,Ma, L..

[12]Penetration of a Single Domain of Bacillus thuringiensis Cry1Ie-Domain I to a Lipid Membrane In vitro. Li Jie,Chen Zhen,He Kang-lai. 2014

[13]Identification of vip3A-type genes from Bacillus thuringiensis strains and characterization of a novel vip3A-type gene. Song, F.,Zhang, J.,Liu, R.,He, K.,Tan, J.,Huang, D.. 2007

[14]Impacts of transgenic cry1Ab rice on non-target planthoppers and their main predator Cyrtorhinus lividipennis (Hemiptera : Miridae) - A case study of the compatibility of Bt rice with biological control. Liu, Zhi-Cheng,Ye, Gong-yin,Shen, Zhi-cheng,Hu, Cui,Peng, Yu-fa,Altosaar, Illimar,Shelton, Anthony M.. 2007

[15]Development of Bt Rice and Bt Maize in China and Their Efficacy in Target Pest Control. Liu, Qingsong,Peng, Yufa,Li, Yunhe,Hallerman, Eric. 2016

[16]Toxicity and binding analyses of Bacillus thuringiensis toxin Vip3A in Cry1Ac-resistant and -susceptible strains of Helicoverpa armigera (Hubner). Lu Qiong,Zhang Yan,Liang Ge-mei,Zhang Qian,Chen Li-zhen. 2015

[17]Use of a pooled clone method to isolate a novel Bacillus thuringiensis Cry2A toxin with activity against Ostrinia furnacalis. Shu, Changlong,Zhang, Jingtao,Chen, Guihua,Liang, Gemei,He, Kanglai,Huang, Dafang,Zhang, Jie,Song, Fuping,Shu, Changlong,Zhang, Jie,Zhang, Jingtao,Huang, Dafang,Crickmore, Neil. 2013

[18]Transgenic cotton co-expressing chimeric Vip3AcAa and Cry1Ac confers effective protection against Cry1Ac-resistant cotton bollworm. Chen, Wen-bo,Liu, Chen-xi,Xiao, Yu-tao,Wu, Kong-ming,Lu, Guo-qing,Cheng, Hong-mei,Xu, Chao,Shen, Zhi-cheng,Soberon, Mario,Bravo, Alejandra.

[19]Identification of metabolism pathways directly regulated by sigma(54) factor in Bacillus thuringiensis. Peng, Qi,Wang, Guannan,Zhang, Jie,Song, Fuping,Liu, Guiming. 2015

[20]Comparative proteomics of Bt-transgenic and non-transgenic cotton leaves. Wang, Limin,Wang, Xuchu,Jin, Xiang,Jia, Ruizong,Huang, Qixing,Tan, Yanhua,Guo, Anping,Wang, Limin. 2015

作者其他论文 更多>>