数字农科院2.0

Base Editing: The Ever Expanding Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Tool Kit for Precise Genome Editing in Plants

文献类型： 外文期刊

作者： Mahmuda Binte Monsur ; Gaoneng Shao +; Yusong Lv ; Shakeel Ahmad ; Xiangjin Wei ; Peisong Hu ; Shaoqing Tang *

作者机构：

关键词： Clustered Regularly Interspaced Short Palindromic Repeat; Base Editor; Point Mutation; Genome Editing; Base Conversion

期刊名称： Genes

ISSN： 2073-4425

年卷期： 2020 年 11 卷 4 期

页码：

收录情况： JCR（2021版）

摘要： Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9), a newly developed genome-editing tool, has revolutionized animal and plant genetics by facilitating modification of target genes. This simple, convenient base-editing technology was developed to improve the precision of genome editing. Base editors generate precise point mutations by permanent base conversion at a specific point, with very low levels of insertions and deletions. Different plant base editors have been established by fusing various nucleobase deaminases with Cas9, Cas13, or Cas12a (Cpf1), proteins. Adenine base editors can efficiently convert adenine (A) to guanine (G), whereas cytosine base editors can convert cytosine (C) to thymine (T) in the target region. RNA base editors can induce a base substitution of A to inosine (I) or C to uracil (U). In this review, we describe the precision of base editing systems and their revolutionary applications in plant science; we also discuss the limitations and future perspectives of this approach.

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