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Ptsi Gene In The Phosphotransfer System Is A Potential Target For Developing A Live Attenuated Salmonella Vaccine

文献类型： 外文期刊

作者： Zhi, Y; Lin, SM; Ahn, KB; Ji, HJ; Guo, HC; Ryu, S; Seo, HS; Lim, S

作者机构：

关键词： Salmonella Enterica Serovar Typhimurium; Phosphotransferase System; Mouse Colitis Model; Auxotrophic Bacterial Vaccine; Humoral Immunity; Cellular Immunity

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

ISSN： 1107-3756

年卷期： 2020 年 45 卷 5 期

页码：

收录情况： JCR（2021版）

摘要： Salmonella enterica serovar Typhimurium causes invasive non-typhoidal Salmonella diseases in animals and humans, resulting in a high mortality rate and huge economic losses globally. As the prevalence of antibiotic-resistant Salmonella has been increasing, vaccination is thought to be the most effective and economical strategy to manage salmonellosis. The present study aimed to investigate whether dysfunction in the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS), which is critical for carbon uptake and survival in macrophages, may be adequate to generate Salmonella-attenuated vaccine strains. A Salmonella strain (KST0555) was generated by deleting the ptsI gene from the PTS and it was revealed that this auxotrophic mutant was unable to efficiently utilize predominant carbon sources during infection (glucose and glycerol), reduced its invasion and replication capacity in macrophages, and significantly (P=0.0065) lowered its virulence in the setting of a mouse colitis model, along with a substantially decreased intestinal colonization and invasiveness compared with its parent strain. The reverse transcription-quantitative PCR results demonstrated that the virulence genes in Salmonella pathogenicity island-1 (SPI-1) and -2 (SPI-2) and the motility of KST0555 were all downregulated compared with its parent strain. Finally, it was revealed that when mice were immunized orally with live KST0555, Salmonella-specific humoral and cellular immune responses were effectively elicited, providing protection against Salmonella infection. Thus, the present promising data provides a strong rationale for the advancement of KST0555 as a live Salmonella vaccine candidate and ptsI as a potential target for developing a live attenuated bacterial vaccine strain.

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