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Interleukin (Il)-21 Promotes The Differentiation Of Iga-Producing Plasma Cells In Porcine Peyer'S Patches Via The Jak-Stat Signaling Pathway

文献类型： 外文期刊

作者： Liu, G; Wang, B; Chen, QB; Li, Y; Li, BY; Yang, N; Yang, SS; Geng, SX; Liu, GL

作者机构：

关键词： Il-21; Iga Class Switch Recombination; B Cell Differentiation; Jak-Stat; Porcine Peyer'S Patches

期刊名称： FRONTIERS IN IMMUNOLOGY

ISSN： 1664-3224

年卷期： 2020 年 11 卷

页码：

收录情况： JCR（2021版）

摘要： Secretory IgA is critical to prevent the invasion of pathogens via mucosa. However, the key factors and the mechanisms of IgA generation in the porcine gut are not well-understood. In this study, a panel of factors, including BAFF, APRIL, CD40L, TGF-beta 1, IL-6, IL-10, IL-17A, and IL-21, were employed to stimulate IgM(+)B lymphocytes from porcine ileum Peyer's patches. The results showed that IL-21 significantly upregulated IgA production of B cells and facilitated cell proliferation and differentiation of antibody-secreting cells. In addition, three transcripts in porcine IgA class switch recombination (CSR), germ-line transcript alpha, post-switch transcript alpha, and circle transcript alpha, were first amplified by (nest-)PCR and sequenced. All these key indicators of IgA CSR were upregulated by IL-21 treatment. Furthermore, we found that IL-21 predominantly activated JAK1, STAT1, and STAT3 proteins and confirmed that the JAK-STAT signaling pathway was involved in porcine IgA CSR. Thus, IL-21 plays an important role in the proliferation and differentiation of IgA-secreting cells in porcine Peyer's patches through the JAK-STAT signaling pathway. These findings provide insights into the mucosal vaccine design by regulation of IL-21 for the prevention and control of enteric pathogens in the pig industry.

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[1]Interleukin (IL)-21 Promotes the Differentiation of IgA-Producing Plasma Cells in Porcine Peyer’s Patches via the JAK-STAT Signaling Pathway. . 2020

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